Avian IgY Antibodies for the Immunoprophylaxis and Therapy of Experimentally Infected Chicken by Salmonella enterica, serovar Enteritidis

Abstract

The possibility of preventing salmonellosis in chicken (pathogen agent — Salmonella enterica, serovar Enteritidis (SE)) by using specific yolk IgY was discussed in presented article. There has been raising the number of reports in the scientific periodicals about increased antibiotic resistance of Salmonella. SE is one of the most common Salmonella serovars worldwide that causes food poisoning in humans. At the same time, infected chicken eggs and meat serve as the main source of infection. For extraction of the IgY, 10 laying hens were used. All layers were hyperimmunized by a vaccine containing live attenuated strains of Salmonella enterica, ser. Enteritidis and Salmonella gallinarum Pullorum. The control group with the same number of layers was not vaccinated. A feed additive for newly hatched chicken and solution with purified and isolated IgY were prepared from egg yolks of immunized and non-immunized birds. During oral administration as a feed additive in per os and intraperitoneal experimental infection by a lethal dose of SE the examination of the efficiency of the obtained product on six-day-old chickens were conducted. Thereby, conducted examinations have shown the high efficiency of the feed additive and preparation with purified immunoglobulins prepared from the yolks of eggs of immunized chicken in experimental infection with a lethal dose of Salmonella Enteritidis.