Avian Influenza A Virus Pandemic Preparedness and Vaccine Development

Vries, Rory D. de; Herfst, Sander; Richard, Mathilde

doi:10.3390/vaccines6030046

Cited by 38 publications

(47 citation statements)

References 103 publications

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“…The highly pathogenic avian influenza (HPAI) viruses of the A/goose/Guangdong/1/1996 (GsGd) lineage have become a major global health concern soon after its emergence in 1996 in China (Xu, Subbarao, & N. J., & Guo, Y., ). Since then, the GsGD lineage of HPAI H5N1 virus has continued to spread globally killing millions of poultry with occasional spill overs to wild birds and mammals, including humans (de Vries, Herfst, & Richard, ). As of 1 November 2018, HPAI H5N1 virus has been responsible for 860 confirmed human infections from 16 countries claiming 454 lives (WHO, ).…”

Section: Introductionmentioning

confidence: 99%

A new reassortant clade 2.3.2.1a H5N1 highly pathogenic avian influenza virus causing recent outbreaks in ducks, geese, chickens and turkeys in Bangladesh

Nooruzzaman

Mumu

Hasnat

et al. 2019

Transbound Emerg Dis

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A total of 15 dead or sick birds from 13 clinical outbreaks of avian influenza in ducks, geese, chickens and turkeys in 2017 in Bangladesh were examined. The presence of H5N1 influenza A virus in the affected birds was detected by RT-PCR. Phylogenetic analysis based on full-length gene sequences of all eight gene segments revealed that these recent outbreaks were caused by a new reassortant of clade 2.3.2.1a H5N1 virus, which had been detected earlier in 2015 during surveillance in live bird markets (LBMs) and wet lands. This reassortant virus acquired PB2, PB1, PA, NP and NS genes from low pathogenic avian influenza viruses mostly of non-H9N2 subtypes but retained HA, NA and M genes of the old clade 2.3.2.1a viruses. Nevertheless, the HA gene of these new viruses was 2.7% divergent from that of the old clade 2.3.2.1a viruses circulated in Bangladesh. Interestingly, similar reassortment events could be traced back in four 2.3.2.1a virus isolates of 2013 from backyard ducks. It suggests that this reassortant virus emerged in 2013, which took two years to be detected at a broader scale (i.e. in LBMs), another two years until it became widely spread in poultry and fully replaced the old viruses. Several mutations were detected in the recent Bangladeshi isolates, which are likely to influence possible phenotypic alterations such as increased mammalian adaptation, reduced susceptibility to antiviral agents and reduced host antiviral response. K E Y W O R D S Bangladesh, clade 2.3.2.1a, domestic poultry, H5N1 HPAIV, new reassortant | 2121 NOORUZZAMAN et Al. S U PP O RTI N G I N FO R M ATI O N Additional supporting information may be found online in the Supporting Information section at the end of the article. How to cite this article: Nooruzzaman M, Mumu TT, Hasnat A, et al. A new reassortant clade 2.3.2.1a H5N1 highly pathogenic avian influenza virus causing recent outbreaks in ducks, geese, chickens and turkeys in Bangladesh. Transbound Emerg Dis.

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Section: Introductionmentioning

confidence: 99%

A new reassortant clade 2.3.2.1a H5N1 highly pathogenic avian influenza virus causing recent outbreaks in ducks, geese, chickens and turkeys in Bangladesh

Nooruzzaman

Mumu

Hasnat

et al. 2019

Transbound Emerg Dis

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“…Highly pathogenic avian influenza (HPAI) H7N9 virus remains global concerns. Current strategies for developing and manufacturing these vaccines are time consuming and somewhat limited in the ability to generate vaccines quickly [15]. Furthermore, conventional influenza vaccines utilizing the HA and NA of influenza viruses have safety and production issues [16].…”

Section: Discussionmentioning

confidence: 99%

Yeast display platform technology to prepare oral vaccine against lethal H7N9 virus challenge in mice

et al. 2020

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Background: Existing methods for preparing influenza vaccines pose the greatest challenge against highly pandemic avian influenza H7N9 outbreak in the poultry and humans. Exploring a new strategy for manufacturing and delivering a safe and effective H7N9 vaccine is needed urgently. Results: An alternative approach is to develop an influenza H7N9 oral vaccine based on yeast display technology in a timely manner. Hemagglutinin (HA) of A/Anhui/1/2013 (AH-H7N9) is used as a model antigen and characterized its expression on the surface of Saccharomyces cerevisiae (S.cerevisiae) EBY 100. Mice administrated orally with S.cerevisiae EBY100/pYD5-HA produced significant titers of IgG antibody as well as significant amounts of cytokines IFN-γ and IL-4. Importantly, S.cerevisiae EBY100/pYD5-HA could provide effective immune protection against homologous A/ Anhui/1/2013 (AH-H7N9) virus challenge. Conclusions: Our findings suggest that platform based on yeast surface technology provides an alternative approach to prepare a promising influenza H7N9 oral vaccine candidate that can significantly shorten the preparedness period and result in effective protection against influenza A pandemic.

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“…Sera were isolated from blood samples collected at day 13 and 28 after the initial immunization, and stored 18 at -20 ℃ until use. Avidin-biotin system (ABS)-enzyme-linked immunosorbent assay (ELISA) was 19 conducted to measure HA-specific IgG titers, as described previously [11]. Briefly, 96-well microplates 20 (Costar, Corning, NY, USA) were coated with 2 μg / mL of recombinant HA protein, and incubated 21 overnight at 4 ℃.…”

Section: Enzyme-linked Immunosorbent Assay (Elisa) 17mentioning

confidence: 99%

Yeast display platform technology to prepare oral vaccine against lethal H7N9 virus challenge in mice

Han

Xie

Gao

et al. 2020

Preprint

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Background Existing methods for preparing influenza vaccines pose the greatest challenge against the threat of highly pandemic avian influenza virus H7N9 outbreak in the poultry and human. Exploring a new strategy for manufacturing and delivering a safe and effective H7N9 vaccine is urgently needed. Results An alternative approach, proposed here, is to develop influenza an oral H7N9 vaccine based on yeast display technology in a timely manner. Hemagglutinin (HA) of A/Anhui/1/2013 (AH-H7N9) as a model antigen and characterized its expression on the surface of Saccharomyces cerevisiae ( S.cerevisiae ) EBY 100. Mice orally administrated with S.cerevisiae EBY100/pYD5-HA produced significant titers of IgG antibody as well as significant amounts of cytokines IFN-γ and IL-4. Importantly, S.cerevisiae EBY100/pYD5-HA could provide complete immune protection against homologous A/Anhui/1/2013 (AH-H7N9) virus challenge. Conclusion Our findings suggest that platform based on yeast surface technology provides an alternative approach to prepare a promising influenza H7N9 vaccine candidate that can significantly shorten the preparedness period and result in effective protection against influenza A pandemic. Keywords: S.cerevisiae EBY100/pYD5-HA, Yeast display technology, Influenza A pandemic.

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Avian Influenza A Virus Pandemic Preparedness and Vaccine Development

Cited by 38 publications

References 103 publications

A new reassortant clade 2.3.2.1a H5N1 highly pathogenic avian influenza virus causing recent outbreaks in ducks, geese, chickens and turkeys in Bangladesh

A new reassortant clade 2.3.2.1a H5N1 highly pathogenic avian influenza virus causing recent outbreaks in ducks, geese, chickens and turkeys in Bangladesh

Yeast display platform technology to prepare oral vaccine against lethal H7N9 virus challenge in mice

Yeast display platform technology to prepare oral vaccine against lethal H7N9 virus challenge in mice

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