Avian pathogenic Escherichia coli - methods for improved diagnosis

Filho, Hiran Castagnino Kunert; Carvalho, Daiane; Grassotti, Tiela Trapp; Soares, Beatriz Dugaich; Rossato, Juliana Marzari; Cunha, Augusto César da; Brito, Kelly Cristina Tagliari de; Cavalli, Lissandra Souto; Brito, Benito Guimarães de

doi:10.1017/s0043933915000264

Cited by 12 publications

(5 citation statements)

References 51 publications

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“…Hence, the European Union has enforced food safety legislation, which usually constitutes a blueprint for the bill in third countries [8,9]. For determining the virulence of E. coli strains, its inoculation into embryos or 1-day-old chicks is followed as a golden standard test [10], whereas serotyping remains the most frequently used diagnostic method in laboratories, although it only allows the identification of a limited number of avian pathogenic E. coli (APEC) strains [11]. Moreover, the prevalence of a certain serotype is linked with the geographical localization of a flock [3]; hence, various molecular typing methods have been employed to study APEC, but none has revealed a specific genotype [12].…”

Section: Introductionmentioning

confidence: 99%

Incidence, Pathotyping, and Antibiotic Susceptibility of Avian Pathogenic Escherichia coli among Diseased Broiler Chicks

et al. 2020

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A total of 54 broiler flocks during the first two weeks of life was used to investigate the incidence of avian pathogenic E. coli in Egypt; 28 isolates (51.85%) were revealed by colony morphology and biochemical identification which then investigated for their serogroups and only 18/28 isolates were serotyped. The most prevalent serotypes were O115, O142, O158, O55, O125, O114, O27, O20, and O15. By application of polymerase chain reaction (PCR), 83.3% (15/18) of the serotyped isolates were confirmed to be E. coli, and 93.3% (14/15), 46.6% (7/15), and 20% (3/15) of isolates harbored the iss, iutA, and fimH genes, respectively. Virulence testing of the selected 13 APEC isolates on the specific-pathogen-free (SPF) chicks revealed them to be highly virulent (15.4%), moderately virulent (23.1%), and avirulent (61.5%); however, all isolates (100%) were extremely virulent towards SPF embryonated chicken eggs. Antibiotic resistance (100% of isolates (n = 13)) was observed for ampicillin, amoxycillin–clavulanic acid, and tetracyclines, colistin (92.31%; 12/13), doxycycline and spiramycin (84.62%; 11/13), florfenicol (69.23%; 9/13), cefotaxime (61.54%; 8/13), and ciprofloxacin (53.85%; 7/13). The highest percentage of sensitivity (53.85% of isolates; 7/13) was recorded for ofloxacin and enrofloxacin followed by gentamycin (46.15%; 6/13). The results suggest that the diagnosis of APEC with PCR is rapid and more accurate than traditional methods for E. coli identification; moreover, the presence or absence of iss, iutA, and/or fimH genes is not an indicator of in vivo pathogenicity of APEC. Thus, further studies, including a wider range of virulence genes and gene sequencing, are required. In addition, serotyping has no effect on the virulence of APEC.

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Section: Introductionmentioning

confidence: 99%

Incidence, Pathotyping, and Antibiotic Susceptibility of Avian Pathogenic Escherichia coli among Diseased Broiler Chicks

et al. 2020

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“…by E. coli. Pathogenic serotypes of E. coli cause a variety of lesions in immunocompromised hosts as well as in poultry (Kunert et al 2015). E. coli coli, Campylobacter sp., Salmonella sp.…”

Section: Introductionmentioning

confidence: 99%

Escherichia coli strains from ostriches and their sensitivity to antimicrobial substances

Ščerbová¹,

Lauková²

2016

Polish Journal of Veterinary Sciences

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Ostriches are bred especially for their high-quality meat. There is a lack of knowledge concerning the ostrich's microflora. Escherichia coli is a commensal microorganism of the poultry intestine, ostriches included. However, some strains may become pathogenic. This study was therefore undertaken to detect coliform bacteria in ostrich faeces and to test their antibiotic profile and sensitivity to enterocins. Faeces (n=54, 18 mixture samples from 3 different age groups of 140 ostriches) were sampled to isolate coliform bacteria. The counts of coliform bacteria varied from 5.69 ± 2.4 log10 CFU/g to 5.73 ± 2.4 CFU/g. Pure colonies were identified using MALDI-TOF MS mass spectrometry and confirmed by phenotypization. Seventy-one strains were allotted to the species E. coli. Sixty-four of those 71 strains caused hemolysis. They were mostly polyresistant to antibiotics. Thirty-two polyresistant strains of E. coli were sensitive to enterocins. These strains were most sensitive to Ent 9296 (26 strains). Moreover, Ent EM41 produced by E. faecium EM41 (isolated from ostrich faeces) inhibited the growth of 20 strains, reaching activity of 100 AU/ml. Our results indicate the possibility of enterocins being used for prevention/reduction of coliforms. Of course, in vivo studies are also being processed.

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“…Therefore, it is important to note that S. x-signatus may act as an occasional reservoir of zoonotic agents because strains of these microorganisms have speci c virulence and resistance characteristics known in humans [39,45]. Multiresistant E. coli and K. pneumoniae are associated with urinary [40], nosocomial [41,42], and avian [43] infections.…”

Section: Resultsmentioning

confidence: 99%

Anthropogenic influence on the skin microbiota of a syntropic anuran in the Atlantic rainforest in northeast Brazil

Santos,

Souza

et al. 2023

Preprint

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Environmental disturbances caused by anthropogenic activities include a loss of habitat and a restructuring of the ecological interactions of biodiversity. Species adapted to the urban ecosystem - synanthropic - can have their cryptic interactions with microorganisms altered. In the case of anurans, the skin bacterial microbiota plays a fundamental role in host protection. However, it is not known how the composition and diversity of the skin microbiota of Scinax x-signatus, a synanthropic anuran, behaves in different fragments. Therefore, this study aimed to compare the composition and diversity of the skin microbiota of Scinax x-signatus in two areas with different degrees of anthropisation in north-eastern Brazil. Eleven individuals of S. x-signatus had their skin microbiota sampled in a control area and an anthropised area of the Recife Botanical Garden (JBR). We reported 21 bacterial isolates that belong to 11 species and 3 families (Enterobacteriaceae, Bacillaceae and Moraxellaceae). Enterobacteria were more frequent, among which we report two species with pathogenic potential found on the skin of treefrogs from the anthropised area - Escherichia coli and Klebsiella pneumoniae. In the control area, Enterobacter hormaechei was an environmental indicator specie. The anthropised area was more diverse than the control area. With the exception of E. coli and K. pneumoniae, the species found have been previously reported as part of the cutaneous microbiota of anurans. The greater diversity of the anthropised area highlights the importance of anthropogenic changes in the emergence of transmission routes for pathogens with zoonotic potential to vertebrates, an indication of environmental quality.

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Avian pathogenic Escherichia coli - methods for improved diagnosis

Cited by 12 publications

References 51 publications

Incidence, Pathotyping, and Antibiotic Susceptibility of Avian Pathogenic Escherichia coli among Diseased Broiler Chicks

Incidence, Pathotyping, and Antibiotic Susceptibility of Avian Pathogenic Escherichia coli among Diseased Broiler Chicks

Escherichia coli strains from ostriches and their sensitivity to antimicrobial substances

Anthropogenic influence on the skin microbiota of a syntropic anuran in the Atlantic rainforest in northeast Brazil

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