Using a novel chemical tritium derivative method, we have determined the base composition of 4S RNA isolated from an RNA tumor virus, the avian myeloblastosis virus, and from normal and neoplastic host cells. Extensive differences were detected, particularly with respect to the amount of methylated bases in the viral RNA. The (7), with respect to the four major bases: adenine, guanine, cytosine, and uracil. No quantitative data on modified ("minor") bases have, however, been reported thus far for 4S RNA from any RNA tumor virus. 4S RNA from AMV does not have the same amino acid-accepting activity as host-cell 4S RNA (2, 3, 6), and lysine tRNA elution profiles have been shown to differ (9).We report in this communication major and minor base constitution data for normal chicken liver, myeloblast, and AMV 4S RNA. Base composition was analyzed by a novel isotope derivative method, which involves the stoichiometric incorporation of tritium label into periodate-oxidized enzymatic digests of RNA, chromatographic resolution of such labeled digests into individual radioactive nucleoside derivatives, and evaluation by liquid-scintillation counting (10)(11)(12)(13)(14). Due to its sensitivity, this method is capable of assaying minute amounts of RNA (<1 Mg) without requiring in vivo labeling of the RNA with isotopes.
MATERIALS AND METHODSVirus Growth and Purification. Avian myeloblastosis virus, Beard's BAI strain A, was propagated by intraperitoneal injection of 1-day-old White Leghorn chickens (15) (Spafas, Inc., Norwich, Conn.). The morphology of peripheral blood was observed by Wright-stained smears. Plasma was obtained by cardiac puncture (heparin was used as an anticoagulant) from chickens with advanced leukemia. The virus was purified by centrifugation of plasma that contained 101I1012 viral particles per ml at 7500 rpm for 10 min in a Sorvall RC-2B refrigerated 40C centrifuge. The supernatant plasma was layered onto the top of 100% glycerol pads and centrifuged at 50C for 90 min at 29,000 rpm in a Spinco no. 30 rotor (16). The virion was collected from the top of the glycerol pad and diluted with 0.1 M NaCl-1 mM Tris (pH 7.4)-i mM EDTA. The virus was purified further after two cycles of differential centrifugation (17).Isolation of Viral RNA. Viral RNA was isolated from purified preparations of AMV by either of two methods. In the first, purified virus from 300 ml of plasma was extracted according to the procedure described by Travnicek (3). The portion of RNA that was soluble in 2 M NaCl was designated as crude viral tRNA (3), which was further purified by Sephadex column chromatography, as described below. A final yield of 120 1g of purified 4S RNA was obtained.The second method for extraction of the viral RNA used a buffering system described by Duesberg (18). Purified viral pellets were obtained from 650 ml of plasma. The second method appears to be preferable because of better preservation of m7G (see Results section). 320 ,ug of purified 4S RNA were obtained after purification by column chromatogr...