Avians as a Model System of Vascular Development

Bressan, Michael; Matsukawa, Takashi

doi:10.1007/978-1-4939-1462-3_14

Cited by 6 publications

(6 citation statements)

References 69 publications

(61 reference statements)

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“…Many ribosomal protein genes were misregulated in the hybrids, suggesting the presence of incompatibilities between chicken- and quail-derived genetic elements that regulate the expression of these ribosomal protein genes. It is unconceivable that the developmental arrest is caused by abnormal sprouting angiogenesis because blood vessels are not formed at the preprimitive streak stages [ 88 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis revealed misregulated gene expression in blastoderms of interspecific chicken and Japanese quail F1 hybrids

et al. 2020

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Hybrid incompatibility, such as sterility and inviability, prevents gene flow between closelyrelated populations as a reproductive isolation barrier. F 1 hybrids between chickens and Japanese quail (hereafter, referred to as quail), exhibit a high frequency of developmental arrest at the preprimitive streak stage. To investigate the molecular basis of the developmental arrest at the preprimitive streak stage in chicken-quail F 1 hybrid embryos, we investigated chromosomal abnormalities in the hybrid embryos using molecular cytogenetic analysis. In addition, we quantified gene expression in parental species and chicken-and quail-derived allele-specific expression in the hybrids at the early blastoderm and preprimitive streak stages by mRNA sequencing. Subsequently, we compared the directions of change in gene expression, including upregulation, downregulation, or no change, from the early blastoderm stage to the preprimitive streak stage between parental species and their hybrids. Chromosome analysis revealed that the cells of the hybrid embryos contained a fifty-fifty mixture of parental chromosomes, and numerical chromosomal abnormalities were hardly observed in the hybrid cells. Gene expression analysis revealed that a part of the genes that were upregulated from the early blastoderm stage to the preprimitive streak stage in both parental species exhibited no upregulation of both chicken-and quail-derived alleles in the hybrids. GO term enrichment analysis revealed that these misregulated genes are involved in various biological processes, including ribosome-mediated protein synthesis and cell proliferation. Furthermore, the misregulated genes included genes involved in early embryonic development, such as primitive streak formation and gastrulation. These results suggest that numerical chromosomal abnormalities due to a segregation failure does not cause the lethality of chicken-quail hybrid embryos, and that the downregulated expression of the genes that are involved in various biological processes, including translation and primitive streak formation, mainly causes the developmental arrest at the preprimitive streak stage in the hybrids.

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Section: Discussionmentioning

confidence: 99%

Transcriptome analysis revealed misregulated gene expression in blastoderms of interspecific chicken and Japanese quail F1 hybrids

et al. 2020

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“…This method of embryo cultivation is more suitable for longer-lasting experiments, since it provides a more physiological environment and the source of calcium for the embryo is kept. It results in fewer developmental abnormalities and the embryos can reach hatching [47,146].…”

Section: In Ovo and Ex Ovo Incubation Methodsmentioning

confidence: 99%

“…On the other hand, the cut window may cause a limitation of the working area, which may cause difficulties in high-resolution microscopy on embryos in ovo. Additionally, the pieces of eggshell spilled on CAM surface pieces may induce angiogenesis [6,47,146].…”

Section: In Ovo and Ex Ovo Incubation Methodsmentioning

confidence: 99%

Chorioallantoic Membrane Models of Various Avian Species: Differences and Applications

Kundeková

Máčajová

Meta

et al. 2021

Biology

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The chorioallantoic membrane model (CAM) of an avian embryo is used as an experimental model in various fields of research, including angiogenesis research and drug testing, xenografting and cancer research, and other scientific and commercial disciplines in microbiology, biochemistry, cosmetics, etc. It is a low-cost, low-maintenance, and well-available in vivo animal model that is non-sentient and can be used as an alternative for other mammal experimental models. It respects the principles of the “3R” rule (Replacement, Reduction, and Refinement)—conditions set out for scientific community providing an essential framework for conducting a more human animal research, which is also in line with constantly raising public awareness of welfare and the ethics related to the use of animal experimental models. In this review, we describe the chorioallantoic membrane of an avian embryo, focusing on its properties and development, its advantages and disadvantages as an experimental model, and the possibilities of its application in various fields of biological research. Since the most common chicken CAM model is already well known and described in many publications, we are particularly focusing on the advantages and application of less known avian species that are used for the CAM model—quail, turkey, and duck.

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“…The chick embryo has established itself within biomedical science as a crucial research model due to its similarity on a cellular and anatomical level to human embryos along with its rapid development, easy visibility and manipulation [1]. The cardiovascular system in the avian develops rapidly with the added benefit of the embryo remaining transparent during morphological development of primary organs, allowing visibility of vasculature formation using simple microscopy techniques [2].…”

Section: Chicken Embryo As An Animal Model For Biomedicinementioning

confidence: 99%

The Effects of Snake Venom (Bitis arietans) on Embryonic Development

Peters¹,

Petrílla²,

Luptáková³

et al. 2021

Medical Toxicology

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Venomous snake bites in pregnant women can lead to poor survival rates in both the foetus and mother; early bites can precipitate teratogenesis, miscarriages, preterm delivery, foetal death and antepartum haemorrhage. The chicken embryo poses as a valuable research model for venom research due to its advantages such as ease of availability, economic feasibility and its non-invasiveness. This study evaluates the embryotoxic effects of Puff adder venom (Bitis arietans) from Namibia, Kenya, South Africa and non-specified region of Africa at varying concentrations. The venoms were applied to chicken embryos on the fourth day of incubation and assessed on a ninth day, focusing on body weight, heart weight, liver weight and mortality rate. Nile blue staining was also performed to observe the occurrence of apoptosis amongst the venoms at the strongest concentrations. The information provided from our results suggested that there was a regional variation in venom toxicity, with the Kenyan venom producing the largest weight changes, whereas the non-specified African venom proved the most lethal across the concentrations. Further studies to assess venom protein concentrations in comparison with regional diet disparities are required.

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Avians as a Model System of Vascular Development

Cited by 6 publications

References 69 publications

Transcriptome analysis revealed misregulated gene expression in blastoderms of interspecific chicken and Japanese quail F1 hybrids

Transcriptome analysis revealed misregulated gene expression in blastoderms of interspecific chicken and Japanese quail F1 hybrids

Chorioallantoic Membrane Models of Various Avian Species: Differences and Applications

The Effects of Snake Venom (Bitis arietans) on Embryonic Development

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