Avicel-adsorbable endoglucanase production by the thermophilic fungus Scytalidium thermophilum type culture Torula thermophila

Arifoğlu, Nazan; Ögel, Zümrüt B.

doi:10.1016/s0141-0229(00)00241-6

Cited by 20 publications

(12 citation statements)

References 20 publications

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“…The amounts of released sugars were quantified using glucose and xylose standards, respectively. The Avicel-adsorbable endoglucanase (AAEG) was assayed as described by Arifog˘lu & Ö gel (2000). The reaction mixture containing 5 ml of sodium acetate buffer (25 mM, pH 5.0), 5 ml of culture supernatant and 1.0 g of Avicel was kept at 4°C for 15 min.…”

Section: Enzyme Assaymentioning

confidence: 99%

“…One of these endoglucanases, characterized as EG-V is known to have a cellulose-binding domain (CBD) and shows Avicel-adsorbable and indigo-binding ability, and this EG-V is important in the textile industry for denim washing (Gusakov et al 2000(Gusakov et al a, 2000b. The AAEG activity is also widespread amongst other type cultures of S. thermophilum namely Torula thermophila (Arifog˘lu & Ö gel, 2000) and Humicola grisea var. thermoidea (Hayashida & Mo, 1986).…”

Section: Effect Of Carbon Sourcesmentioning

confidence: 99%

“…thermoidea (Hayashida & Mo, 1986). Arifog˘lu & Ö gel (2000) reported that 74% of endoglucanase as AAEG activity was produced by T. thermophila under submerged fermentation.…”

Section: Effect Of Carbon Sourcesmentioning

confidence: 99%

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Optimization of Medium Components for Production of Cellulases by Melanocarpus sp. MTCC 3922 under Solid-state Fermentation

Jatinder

Chadha

Saini

2006

World J Microbiol Biotechnol

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The medium components for the production of extracellular cellulases by Melanocarpus sp. MTCC 3922 were optimized using solid-state fermentation. Melanocarpus sp. cultured in optimized medium containing 1.5% urea, and 0.12% KH 2 PO 4 along with a trace element solution and surfactant (Tween 20), produced endoglucanase (142.4 U/g of substrate), Avicel-adsorbable endoglucanase (27.0 U/g of substrate), Avicelase (0.65 U/ g of substrate), FPase (39.9 U/g of substrate) and b-glucosidase (109.0 U/g of substrate) activities. The presence of sulphate ions in traces stimulated endoglucanase yields. The IEF fractionation of the crude proteins from Melanocarpus sp. showed the expression of 3, 1 and 11 isoforms of endoglucanase, b-glucosidase and xylanase, respectively.

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Section: Enzyme Assaymentioning

confidence: 99%

Section: Effect Of Carbon Sourcesmentioning

confidence: 99%

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Optimization of Medium Components for Production of Cellulases by Melanocarpus sp. MTCC 3922 under Solid-state Fermentation

Jatinder

Chadha

Saini

2006

World J Microbiol Biotechnol

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“…There are at least three categories of enzymes that convert cellulose (a linear, typically insoluble polymer containing thousands of glucose units) into soluble sugars [6]. The cellulolytic complex includes the following: endoglucanase (EG, EC 3.2.1.4), which randomly catalyzes the hydrolysis internal β -1,4 glycosidic bonds in the cellulose chain; cellobiohydrolase (CBH, EC 3.2.1.91), which moves progressively along the cellulose chain and catalyzes the release of cellobiose units from the chain's terminus; and β -glucosidase (BG, EC 3.2.1.21), which converts cellobiose and soluble cellodextrins into glucose.…”

Section: Introductionmentioning

confidence: 99%

Optimisation of Cellulase Production by Penicillium funiculosum in a Stirred Tank Bioreactor Using Multivariate Response Surface Analysis

Carvalho

Gomes

et al. 2014

Enzyme Research

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Increasing interest in the production of second-generation ethanol necessitates the low-cost production of enzymes from the cellulolytic complex (endoglucanases, exoglucanases, and β-glucosidases), which act synergistically in cellulose breakdown. The present work aimed to optimise a bioprocess to produce these biocatalysts from the fungus Penicillium funiculosum ATCC11797. A statistical full factorial design (FFD) was employed to determine the optimal conditions for cellulase production. The optimal composition of culture media using Avicel (10 g·L−1) as carbon source was determined to include urea (1.2 g·L−1), yeast extract (1.0 g·L−1), KH2PO4 (6.0 g·L−1), and MgSO4 ·7H2O (1.2 g·L−1). The growth process was performed in batches in a bioreactor. Using a different FFD strategy, the optimised bioreactor operational conditions of an agitation speed of 220 rpm and aeration rate of 0.6 vvm allowed the obtainment of an enzyme pool with activities of 508 U·L−1 for FPase, 9,204 U·L−1 for endoglucanase, and 2,395 U·L−1 for β-glucosidase. The sequential optimisation strategy was effective and afforded increased cellulase production in the order from 3.6 to 9.5 times higher than production using nonoptimised conditions.

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“…Levels of significance p < 0.005*** p < 0.05** p < 0.5* Humicola insolens where endoglucanase characterized as EG-V is known to have CBD and showed Aviceladsorbable and indigo binding ability which is important in textile industry for denim wash (Gusakov et al 2000a, b). The AAEG activity is also wide spread amongst other type cultures of S. thermophilum namely Torula thermophila (Arifog˘lu & Ö gel 2000) and Humicola grisea var. thermoidea (Hayashida 1986).…”

Section: Avicel-adsorbable Endoglucanase (Aaeg)mentioning

confidence: 98%

Optimization of culture conditions for production of cellulases and xylanases by Scytalidium thermophilum using Response Surface Methodology

Jatinder

Chadha

Saini

2006

World J Microbiol Biotechnol

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Scytalidium thermophilum type culture Humicola insolens MTCC 4520 isolated from composting soil was optimized for production of cellulolytic and hemicellulolytic enzymes (endoglucanase, Avicel-adsorbable endoglucanase, FPase, b-glucosidase, xylanase and mannanase) by solid-state fermentation (SSF). Initial experiments showed that culture medium containing rice straw and wheat bran (1:3) as carbon source prepared in a synthetic basal medium supported maximal enzyme production at 45°C. Further optimization of enzyme production was carried out using Box-Behnken design of experiments to study the influence of process variables (inoculum level, (NH 4 ) 2 SO 4 and pH) on enzyme production. The response surface plots revealed the conditions for obtaining optimal enzyme levels. The models computed for R 2 value ranged between 95% and 98.7% indicating they are appropriate and can be useful to predict the effect of inoculum level, (NH 4 ) 2 SO 4 and pH on enzyme production. Under optimized conditions 62.5 ± 0.50, 23.0 ± 0.58, 3.0 ± 0.50, 151. 00 ± 8.194, 196 ± 5.033 and 4.9 ± 0.32 (units/g substrate) of endoglucanase (EG), Avicel-adsorbable endoglucanase (AAEG), FPase, b-glucosidase, xylanase and mannanase were produced, respectively. Isoelectric focusing (IEF) of the crude extract showed that S. thermophilum produced six different EG isoforms, of which the EG corresponding to pI values of 8.4, 7.9 and 6.5 showed affinity for Avicel, thereby indicating the presence of a cellulose-binding domain (CBD). Furthermore, seven isoforms of b-glucosidase and ten multiple forms of xylanase distributed over a wide range of pI were also detected.

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Avicel-adsorbable endoglucanase production by the thermophilic fungus Scytalidium thermophilum type culture Torula thermophila

Cited by 20 publications

References 20 publications

Optimization of Medium Components for Production of Cellulases by Melanocarpus sp. MTCC 3922 under Solid-state Fermentation

Optimization of Medium Components for Production of Cellulases by Melanocarpus sp. MTCC 3922 under Solid-state Fermentation

Optimisation of Cellulase Production by Penicillium funiculosum in a Stirred Tank Bioreactor Using Multivariate Response Surface Analysis

Optimization of culture conditions for production of cellulases and xylanases by Scytalidium thermophilum using Response Surface Methodology

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