Avicularin alleviates osteoporosis-induced implant loosening by attenuating macrophage M1 polarization via its inhibitory effect on the activation of NF-κB

Yang, Yunshang; Dong, Shuai; Shi, Jiandong; Xiao, Long; Wang, Zhifang; Yin, Zhengyu; Zhuang, Qi; Chen, Shuangshuang; Li, Yajun; Gu, Yu; Zhu, Jie; Geng, Dechun; Wang, Zhirong

doi:10.1016/j.biopha.2022.114113

Cited by 13 publications

(3 citation statements)

References 43 publications

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“…The NF-κB signaling pathway has been confirmed to be a classic pathway related to immunity and inflammation. It also regulates macrophage polarization [44] and produces pro-inflammatory cytokines that recruit more neutrophils, monocytes, and lymphocytes, thereby exacerbating the host inflammatory response [34,45]. In the present study, in the IHC staining of mouse colon tissue, ART treatment inhibited the activation of M1-type macrophages (F4/80, CD11c), as well as significantly reduced the levels of M1-type macrophage markers (iNOS, CD11c) and chemokines (CCL4); this may suggest that ART can selectively attenuate M1-type polarization of pro-inflammatory macrophages to further reduce intestinal inflammation.…”

Section: Discussionmentioning

confidence: 99%

Artemether Attenuates Gut Barrier Dysfunction and Intestinal Flora Imbalance in High-Fat and High-Fructose Diet-Fed Mice

Ren,

Xu,

et al. 2023

Nutrients

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Intestinal inflammation is a key determinant of intestinal and systemic health, and when our intestines are damaged, there is disruption of the intestinal barrier, which in turn induces a systemic inflammatory response. However, the etiology and pathogenesis of inflammatory diseases of the intestine are still not fully understood. Artemether (ART), one of the artemisinin derivatives, has been widely used to treat malaria. Nevertheless, the effect of ART on intestinal inflammation remains unclear. The present study intended to elucidate the potential mechanism of ART in diet-induced intestinal injury. A high-fat and high-fructose (HFHF) diet-induced mouse model of intestinal injury was constructed, and the mice were treated with ART to examine their role in intestinal injury. RT-qPCR, Western blotting, immunohistochemical staining, and 16S rRNA gene sequencing were used to investigate the anti-intestinal inflammation effect and mechanism of ART. The results indicated that ART intervention may significantly ameliorate the intestinal flora imbalance caused by the HFHF diet and alleviate intestinal barrier function disorders and inflammatory responses by raising the expression of tight junction proteins ZO-1 and occludin and decreasing the expression of pro-inflammatory factors TNF-α and IL-1β. Moreover, ART intervention restrained HFHF-induced activation of the TLR4/NF-κB p65 pathway in colon tissue, which may be concerned with the potential protective effect of ART on intestinal inflammation. ART might provide new insights into further explaining the mechanism of action of other metabolic diseases caused by intestinal disorders.

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Section: Discussionmentioning

confidence: 99%

Artemether Attenuates Gut Barrier Dysfunction and Intestinal Flora Imbalance in High-Fat and High-Fructose Diet-Fed Mice

Ren,

Xu,

et al. 2023

Nutrients

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“…In addition, we have supplemented western blotting (WB) experiments on relevant pathways to demonstrate the results of RNA sequencing. The results (Figure S14, Supporting Information) showed that in the treatment group of RAW 264.7 cells, the p-p65/p65 ratio in pathway decreased, indicating that the ex-pression of NF-𝜅B pathway [40] was inhibited by CuBA nanoparticles. In MC3T3 cells co cultured with CuBA nanoparticles (Figure S15, Supporting Information), the p53 related pathway was inhibited, [41] indicating that CuBA nanoparticles could promote osteogenic differentiation of MC3T3 cells.…”

Section: Molecular Mechanism Underlying the Anti-inflammatory And Pro...mentioning

confidence: 99%

Fenton‐like Reaction Inspired “·OH Catalyzed” Osteogenic Process for the Treatment of Osteoporosis

Wang,

Ye,

Yang

et al. 2024

Adv Healthcare Materials

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Inspired by the Fenton‐like reaction, we combined copper peroxide (CP) nanoparticles with black phosphorus (BP) nanosheets to form a hydroxyl radical (·OH)‐centered “catalytic” osteogenic system. CP‐produced ·OH interacts with BP to rapidly produce a large amount of phosphate ions, thus accelerating self‐mineralization and promoting bone formation. In turn, BP not only exerts anti‐inflammatory effects, thereby providing a favorable microenvironment for bone formation, but also offsets the potential toxicity of CP induced by reactive oxygen species (ROS). Together with copper ions (Cu2+), phosphate ions are also released as a byproduct of this process, which can contribute to the comprehensive promotion of osteogenesis.This article is protected by copyright. All rights reserved

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“…Specifically, it inhibits the polarization of M1 cells through NF-κB inhibition, which helps to attenuate the inflammatory bone loss caused by macrophages of M1 phenotype. 108 Chronic inflammation, a key driver of bone loss, is closely linked to macrophage polarization, and an imbalance in macrophage polarization leads to an accumulation of inflammatory factors, affecting bone metabolic homeostasis and exacerbating the development of bone loss. Therefore, targeting macrophage polarization to induce a dynamic M1/M2 balance could be a novel therapeutic strategy for OP.…”

Section: Macrophage Therapy For Diseases Associated With An Imbalance...mentioning

confidence: 99%

Macrophage Polarization and the Regulation of Bone Immunity in Bone Homeostasis

Hu,

Shang,

Yang

et al. 2023

JIR

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Bone homeostasis is a dynamic equilibrium state of bone formation and absorption, ensuring skeletal development and repair. Bone immunity encompasses all aspects of the intersection between the skeletal and immune systems, including various signaling pathways, cytokines, and the crosstalk between immune cells and bone cells under both homeostatic and pathological conditions. Therefore, as key cell types in bone immunity, macrophages can polarize into classical pro-inflammatory M1 macrophages and alternative anti-inflammatory M2 macrophages under the influence of the body environment, participating in the regulation of bone metabolism and playing various roles in bone homeostasis. M1 macrophages can not only act as precursors of osteoclasts (OCs), differentiate into mature OCs, but also secrete pro-inflammatory cytokines to promote bone resorption; while M2 macrophages secrete osteogenic factors, stimulating the differentiation and mineralization of osteoblast precursors and mesenchymal stem cells (MSCs), and subsequently increase bone formation. Once the polarization of macrophages is imbalanced, the resulting immune dysregulation will cause inflammatory stimulation, and release a large amount of inflammatory factors affecting bone metabolism, leading to pathological conditions such as osteoporosis (OP), rheumatoid arthritis (RA), and steroid-induced femoral head necrosis (SANFH). In this review, we introduce the signaling pathways and related factors of macrophage polarization, as well as their relationships with immune factors, OB, OC, and MSC. We also discuss the roles of macrophage polarization and bone immunity in various diseases of bone homeostasis imbalance, as well as the factors regulating them, which may help to develop new methods for treating bone metabolic disorders.

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Avicularin alleviates osteoporosis-induced implant loosening by attenuating macrophage M1 polarization via its inhibitory effect on the activation of NF-κB

Cited by 13 publications

References 43 publications

Artemether Attenuates Gut Barrier Dysfunction and Intestinal Flora Imbalance in High-Fat and High-Fructose Diet-Fed Mice

Artemether Attenuates Gut Barrier Dysfunction and Intestinal Flora Imbalance in High-Fat and High-Fructose Diet-Fed Mice

Fenton‐like Reaction Inspired “·OH Catalyzed” Osteogenic Process for the Treatment of Osteoporosis

Macrophage Polarization and the Regulation of Bone Immunity in Bone Homeostasis

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