Avidin:  A Natural Bridge for Quantum Dot-Antibody Conjugates

Goldman, Ellen R.; Balighian, Eric; Mattoussi, Hedi; Kuno, Masaru; Mauro, J. Matthew; Tran, Phan T.; Anderson, George P.

doi:10.1021/ja0125570

Cited by 502 publications

(391 citation statements)

References 22 publications

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“…The conjugation of MPA-capped aqQDs with avidin was performed as previously reported [10,35,36]. Avidin, a positively charged protein, is able to interact with the negatively charged MPA-capped QDs.…”

Section: Preparation and Validation Of Avidin Linked Aqqds Probesmentioning

confidence: 99%

A nano- and micro- integrated protein chip based on quantum dot probes and a microfluidic network

Yan

et al. 2008

Nano Res.

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A novel nano-and micro-integrated protein chip (NMIPC) that can detect proteins with ultrahigh sensitivity has been fabricated. A microfl uidic network (μFN) was used to construct the protein chips, which allowed facile patterning of proteins and subsequent biomolecular recognition. Aqueous phase-synthesized, water-soluble fl uorescent CdTe/CdS core-shell quantum dots (aqQDs), having high quantum yield and high photostability, were used as the signaling probe. Importantly, it was found that aqQDs were compatible with microfluidic format assays, which afforded highly sensitive protein chips for cancer biomarker assays.

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Section: Preparation and Validation Of Avidin Linked Aqqds Probesmentioning

confidence: 99%

A nano- and micro- integrated protein chip based on quantum dot probes and a microfluidic network

Yan

et al. 2008

Nano Res.

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“…NaEuF 4 NPs with a mean size of ≈8.5 nm (≈4300 Eu 3+ ions per NP) were synthesized and employed as the DELBA nanoprobes after conjugation with biotin. The biotinylated nanoprobes can indirectly capture the biotinylated biomolecules through the bridging of avidin 32. By virtue of the specific recognition of the anti‐CEA antibody with CEA, a sandwich‐type DELBA was built with the labeling of the biotinylated NaEuF 4 nanoprobes.…”

Section: Ultrasensitive In Vitro Detection Of Tumor Markersmentioning

confidence: 99%

Ultrasensitive Luminescent In Vitro Detection for Tumor Markers Based on Inorganic Lanthanide Nano‐Bioprobes

Zheng

Zhou

et al. 2016

Advanced Science

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Ultrasensitive and accurate detection of tumor markers is of vital importance for the screening or diagnosis of cancers at their early stages and for monitoring cancer relapse after surgical resection. Inorganic lanthanide (Ln3+) nanoparticles (NPs), owing to their superior physicochemical characteristics, are regarded as a new generation of luminescent nano‐bioprobes in the field of cancer diagnosis and therapy. In this progress report, a focus is set on our recent efforts on the development of inorganic Ln3+‐NPs as efficient luminescent nano‐bioprobes for the ultrasensitive in vitro biodetection of tumor markers, with an emphasis on the dissolution‐enhanced luminescent bioassay (DELBA), an emerging technique recently developed toward practical medical applications.

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“…Most QD-based biosensors have used antibodies to target specific proteins of interest. [25][26][27][28] Although the specificity is very high, the large size (20-50 nm) of the conjugated antibody is a frequent concern. [29,30] Another approach is to attach a relatively small lipid to the QD directly to confer binding, [31] however the challenge is to retain aqueous solubility of the modified QD.…”

Section: Introductionmentioning

confidence: 99%

Mixed-surface, lipid-tethered quantum dots for targeting cells and tissues

Zhang

Haage

Whitley

et al. 2012

Colloids and Surfaces B: Biointerfaces

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Quantum dots (QDs), with their variable luminescent properties, are rapidly transcending traditional labeling techniques in biological imaging and hold vast potential for biosensing applications. An obstacle in any biosensor development is targeted specificity. Here we report a facile procedure for creating QDs targeted to the cell membrane with the goal of cell-surface protease biosensing. This procedure generates water-soluble QDs with variable coverage of lipid functional groups. The resulting hydrophobicity is quantitatively controlled by the molar ratio of lipids per QD. Appropriate tuning of the hydrophobicity ensures solubility in common aqueous cell culture media and while providing affinity to the lipid bilayer of cell membranes. The reaction and exchange process was directly evaluated by measuring UV-vis absorption spectra associated with dithiocarbamate formation. Cell membrane binding was assessed using flow cytometry and total internal reflection fluorescence imaging with live cells, and tissue affinity was measured using histochemical staining and fluorescence imaging of frozen tissue sections. Increases in cell and tissue binding were found to be regulated by both QD hydrophobicity and surface charge, underlying the importance of QD surface properties in the optimization of both luminescence and targeting capability. the hydrophobicity ensures solubility in common aqueous cell culture media and while providing affinity to the lipid bilayer of cell membranes. The reaction and exchange process was directly evaluated by measuring UV-vis absorption spectra associated with dithiocarbamate formation. Cell membrane binding was assessed using flow cytometry and total internal reflection fluorescence imaging with live cells, and tissue affinity was measured using histochemical staining and fluorescence imaging of frozen tissue sections. Increases in cell and tissue binding were found to be regulated by both QD hydrophobicity and surface charge, underlying the importance of QD surface properties in the optimization of both luminescence and targeting capability. Mixed-surface, Lipid-tethered Quantum Dots for Targeting Cells and Tissues

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Avidin: A Natural Bridge for Quantum Dot-Antibody Conjugates

Cited by 502 publications

References 22 publications

A nano- and micro- integrated protein chip based on quantum dot probes and a microfluidic network

A nano- and micro- integrated protein chip based on quantum dot probes and a microfluidic network

Ultrasensitive Luminescent In Vitro Detection for Tumor Markers Based on Inorganic Lanthanide Nano‐Bioprobes

Mixed-surface, lipid-tethered quantum dots for targeting cells and tissues

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