Avidin Adsorption to Silk Fibroin Films as a Facile Method for Functionalization

Abbott, Alycia; Oxburgh, Leif; Kaplan, David L.; Coburn, Jeannine M.

doi:10.1021/acs.biomac.8b00824

Cited by 22 publications

(16 citation statements)

References 62 publications

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“…Silk has been widely used to support growth of cornea, gastrointestinal tissues, and neural tissue due to its properties of biocompatibility, safety and adjustable degradability and its ability to be modified by incorporation of macromolecules. Recently, avidin was incorporated into the silk material, thus biotinylated proteins (e.g., growth factors) can be conjugated to increase their local concentration, which could possibly provide instructive signals to guide tissue morphogenesis in the scaffold . Because the compartments in silk scaffolds have variable sizes and shapes leading to poorly organized kidney tubules in this study, the development of silk‐based 3D scaffold may be desirable.…”

Section: Hydrogels In Organoids Formationmentioning

confidence: 99%

Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip

et al. 2019

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The major motivation is to better mimic human physiology and functions at multiscales from the molecular to the cellular, tissue, organ or even whole organism level. Current model systems primarily rely on the monolayer cell cultures and animal models. Simplistic monolayer cultures have their advantages, but they are often significantly different in gene expression, epigenetics, and cell function compared to native 3D tissues. [1,2] Also, they often lack cell-cell and cell-matrix interactions, [2,3] leading to the absence of tissue specific properties. Although animal models are widely used in biomedical research, they fail to faithfully predict human responses in a physiologically relevant manner due to the significant species divergences. [4] The limitations of these systems have provided an impetus for the development of alternative cell-based 3D models in vitro that better resemble the complex functionalities of living organs. Over the last several years, organoids and organ-on-a-chip (OOC), representing the major technological breakthrough, have emerged as two distinct model systems to achieve the same goal of building 3D organotypic models in vitro by bridging the gap between animal models and monolayer cultures. These engineered models are different in terms of cell source, tissue composition, architectural variability, functional features, scales, cellular fidelity, etc. Organoids, primarily evolving from developmental principles, refer to 3D multicellular tissues by self-organization of stem cells or organ-specific progenitors, which can recapitulate the intricate architectures and functionalities of in vivo organs. [5][6][7] Recent breakthroughs in organoid technology have enabled the successful generation of a variety of human organoids, such as the brain, [8,9] intestine, [10,11] liver, [12] kidney, [13,14] lung, [15] etc. These near physiological 3D organoids have garnered momentum for their potential applications in human organ development and disease modeling, drug screening and regenerative medicine. The explosion of organoids research has been catalyzed by the significant progress of stem cell biology and availability of human stem cells. In contrast, the OOC, relying on the bioengineering design principle, is a miniaturized in vitro model that can recreate the functional units of living organs on a microfluidic cell culture device using predifferentiated cells, often cell lines. [1,16] The OOC is primarily evolved from the convergence of tissue engineering and microfabricated technologies, which is characterized by the capability to simulate cellular microenvironment by precise control over fluid flow, mechanical forces and biochemical factors. [4,17] Remarkable progress has been made Significant advances in materials, microscale technology, and stem cell biology have enabled the construction of 3D tissues and organs, which will ultimately lead to more effective diagnostics and therapy. Organoids and organs-on-a-chip (OOC), evolved from developmental biology and bioengineering principles, have e...

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Section: Hydrogels In Organoids Formationmentioning

confidence: 99%

Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip

et al. 2019

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“…Because of its biocompatibility, hydrophilic/hydrophobic properties, and ease of fabrication, silk fibroin (SF) carriers such as microspheres, films, , hydrogels, , and scaffolds , were developed to release growth factors, vitamins, macromolecules, and anticancer agents. Recently, SF nanofibers (SFNs) were assembled to facilitate tissue regeneration and drug loading because of the loading capacity. , DFO was loaded on SFNs to form injectable hydrogels .…”

Section: Introductionmentioning

confidence: 99%

Injectable Desferrioxamine-Laden Silk Nanofiber Hydrogels for Accelerating Diabetic Wound Healing

Ding

Zhang

Guo

et al. 2021

ACS Biomater. Sci. Eng.

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Dysangiogenesis and chronic inflammation are two critical reasons for diabetic foot ulcers. Desferrioxamine (DFO) was used clinically in the treatment of diabetic foot ulcers by repeated injections because of its capacity to induce vascularization. Biocompatible carriers that release DFO slowly and facilitate healing simultaneously are preferable options to accelerate the healing of diabetic wounds. Here, DFO-laden silk nanofiber hydrogels that provided a sustained release of DFO for more than 40 days were used to treat diabetic wounds. The DFO-laden hydrogels stimulated the healing of diabetic wounds. In vitro cell studies revealed that the DFO-laden hydrogels modulated the migration and gene expression of endothelial cells, and they also tuned the inflammation behavior of macrophages. These results were confirmed in an in vivo diabetic wound model. The DFO-laden hydrogels alleviated dysangiogenesis and chronic inflammation in the diabetic wounds, resulting in a more rapid wound healing and increased collagen deposition. Both in vitro and in vivo studies suggested potential clinical applications of these DFO-laden hydrogels in the treatment of diabetic ulcers.

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“…Strategies explored for protein immobilization have mainly relied on its nonselective adsorption or entrapment, but this method is difficult to maintain the local concentration due to diffusion, degeneration, cell uptake, and noggin inhibition. − Alternatively, covalent immobilization by functional groups present in protein structure such as amines − and thiols − is efficient to prevent the molecules from diffusion or rapid degeneration. However, this nonselective immobilization cannot control the orientation and conformation of proteins, which is crucial for the modulation of cellular behavior. , Recently, site-specific immobilization of proteins has been proved to be a promising way to provide higher retention of bioactivity by favoring the access to the active sites of immobilized proteins, such as biorthogonal chemical reactions, , enzymatic ligation, , and affinity binding. − …”

Section: Introductionmentioning

confidence: 99%

“…However, the successful implementation of affinity binding is strongly dependent on the appropriate selection of the binding pairs. The high-affinity interactions, such as streptavidin and biotin, , host–guest, , and coiled-coil, , although often used to build binding systems, require the protein to be affinity tagged either by the recombination technique or chemical modification . In contrast, direct use of binding sites on the natural protein structure is more simple and represents a tag-free alternative since the noncovalent interactions could be generated without the need for tedious protein modification.…”

Section: Introductionmentioning

confidence: 99%

Tag-Free Site-Specific BMP-2 Immobilization with Long-Acting Bioactivities via a Simple Sugar–Lectin Interaction

Wang

Zhang

et al. 2020

ACS Biomater. Sci. Eng.

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The construction of a biomaterial matrix with biological properties is of great importance to developing functional materials for clinical use. However, the site-specific immobilization of growth factors to endow materials with bioactivities has been a challenge to date. Considering the wide existence of glycosylation in mammalian proteins or recombinant proteins, we establish a bioaffinity-based protein immobilization strategy (bioanchoring method) utilizing the native sugar–lectin interaction between concanavalin A (Con A) and the oligosaccharide chain on glycosylated bone morphogenetic protein-2 (GBMP-2). The interaction realizes the site-specific immobilization of GBMP-2 to a substrate modified with Con A while preserving its bioactivity in a sustained and highly efficient way, as evidenced by its enhanced ability to induce osteodifferentiation compared with that of the soluble GBMP-2. Moreover, the surface with Con A-bioanchored GBMP-2 can be reused to stimulate multiple batches of C2C12 cells to differentiate almost to the same degree. Even after 4 month storage at 4 °C in phosphate-buffered saline (PBS), the Con A-bioanchored GBMP-2 still maintains the bioactivity to stimulate the differentiation of C2C12 cells. Furthermore, the ectopic ossification test proves the in vivo bioactivity of bioanchored GBMP-2. Overall, our results demonstrate that the tag-free and site (i.e., sugar chain)-specific protein immobilization strategy represents a simple and generic alternative, which is promising to apply for other glycoprotein immobilization and application. It should be noted that although the lectin we utilized can only bind to d-mannose/d-glucose, the diversity of the lectin family assures that a specific lectin could be offered for other sugar types, thus expanding the applicable scope further.

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Avidin Adsorption to Silk Fibroin Films as a Facile Method for Functionalization

Cited by 22 publications

References 62 publications

Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip

Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip

Injectable Desferrioxamine-Laden Silk Nanofiber Hydrogels for Accelerating Diabetic Wound Healing

Tag-Free Site-Specific BMP-2 Immobilization with Long-Acting Bioactivities via a Simple Sugar–Lectin Interaction

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