Avocado soybean unsaponifiables ameliorates cartilage and subchondral bone degeneration in mono-iodoacetate-induced knee osteoarthritis in rats

Alafify, Aliaa Salah Ali; El–Akabawy, Gehan; El-Sherif, Neveen M.; El-Safty, Fatma El-Nabawya A.; El-Habiby, Mostafa M

doi:10.1016/j.tice.2018.05.001

Cited by 23 publications

(12 citation statements)

References 36 publications

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“…A Chinese cross-sectional study conducted by Li et al [18] also showed that dietary intake of soy milk, a stable emulsion of water,oil, and soy protein, was negatively associated with the osteophyte formation in knee joint. The effectiveness and safety of soy unsaponifiables, as symptomatic slow acting agents for OA, have been demonstrated in clinical studies of knee OA [21]. As an isoflavone in soybeans and soy products, genistein could suppress the production of proinflammatory molecules and increase the synthesis of glycosaminoglycans in chondrocytes [22].…”

Section: Discussionmentioning

confidence: 99%

Protective effect of ethyl acetate fraction from Semen sojae germinatum, the processed sprout of Chinese black soybean, on rat experimental osteoarthritis

Wang

Guo

et al. 2020

BMC Complement Med Ther

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Background: Our previous in vitro study reported that the ethyl acetate fraction (EAF) of Semen sojae germinatum (SSG), the processed sprout of Chinese black soybean, possessed the potent anti-inflammatory and chondroprotective properties. The aim of the present work was to verify the in vivo antiosteoarthritic effect of EAF from SSG on a rat osteoarthritis (OA) model. Methods: A classical rat OA model was surgically induced by anterior cruciate ligament transaction (ACLT). The OA rats were intra-articularly administered EAF from SSG for 8 weeks. The cartilage and synovial tissues were stained with hematoxylin and eosin (HE) to observe the histopathological changes. Safranin O/fast green staining was used to assess the glycosaminoglycan content in cartilage tissue sections. The expression of type II collagen and matrix metalloproteinase (MMP)-13 in cartilage was measured by immunohistochemistry. The apoptotic chondrocytes in the cartilage sections were detected using TUNEL assay. The concentrations of interleukin (IL)-1β and tumor necrosis factor (TNF)-ɑ in synovial fluid were determined using ELISA. Results: Intra-articular administration of EAF from SSG well retained the structure and superficial layer of cartilage tissues, ameliorated cartilage lesion and the degradation of cartilage matrix, including proteoglycan and type II collagen, induced by ACLT operation. The ACLT-induced upregulation of MMP-13 expression in the cartilage tissues was resisted by EAF from SSG. Moreover, EAF from SSG inhibited the ACLT-induced chondrocyte apoptosis. Compared to OA model group, the inflammatory status of synovial membrane was improved, the levels of inflammatory cytokines IL-1β and TNF-ɑ in synovial fluid were decreased in rats administrated with EAF from SSG. Conclusion: These data suggested that EAF from SSG displayed in vivo protective effect on OA development via preventing the degeneration of articular cartilage, inhibiting chondrocyte apoptosis and suppressing synovial inflammation.

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Section: Discussionmentioning

confidence: 99%

Protective effect of ethyl acetate fraction from Semen sojae germinatum, the processed sprout of Chinese black soybean, on rat experimental osteoarthritis

Wang

Guo

et al. 2020

BMC Complement Med Ther

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“…Currently, non-steroidal anti-inflammatory drugs (NSAIDS) and steroids are being used as a first-line therapy against OA to control pain and inflammation [9]. However, their prolonged use in elderly is associated with adverse health consequences, such as gastro-intestinal, cardiovascular, and urinary disorders [10,11]. An effective medicine with less or no side effects is immensely needed for the treatment of OA.…”

Section: Introductionmentioning

confidence: 99%

Andrographis paniculata Extract Relieves Pain and Inflammation in Monosodium Iodoacetate-Induced Osteoarthritis and Acetic Acid-Induced Writhing in Animal Models

Lee

Baek

Hwang³

et al. 2020

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Osteoarthritis (OA), being the most prominent degenerative joint disease is affecting millions of elderly people worldwide. Although Andrographis paniculata is an ethnic medicine with a long history of being used as analgesic agent, no study using a monosodium iodoacetate (MIA) model has investigated its potential activities against OA. In this study, experimental OA was induced in rats with a knee injection of MIA, which represents the pathological characteristics of OA in humans. A. paniculata extract (APE) substantially reversed the loss of hind limb weight-bearing and the cartilage damage resulted from the OA induction in rats. Additionally, the levels of serum pro-inflammatory cytokines, such as IL-1β, IL-6, and TNF-α as well as the concentration of matrix metalloproteinases, including MMP-1, MMP-3, MMP-8, and MMP-13 were decreased by APE administration. Acetic acid-induced writhing responses in mice which quantitatively measure pain were significantly reduced by APE. In vitro, APE inhibited the generation of NO and downregulated the expression of IL-1β, IL-6, COX-2, and iNOS in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The above results suggest the potential use APE as a therapeutic agent against OA.

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“…A number of studies evaluated the anti‐inflammatory effects of Arthrocen in management of osteoarthritis via inhibiting the activity and expression of pro‐inflammatory cytokines such as IL‐1β and TNF‐α, blocking nuclear translocation of NF‐κB and chemokines (IL‐8), and inhibiting the activation of COX‐2 (Al‐Afify, El‐Akabawy, El‐Sherif, El‐Safty, & El‐Habiby, 2018; DiNubile, 2010; Ownby et al., 2014). Another study found that Arthrocen had an anti‐inflammatory role in an experimental model of inflammation due to presence of its phytosterol constituents, which have both antioxidant and analgesic actions (de Jong et al., 2003).…”

Section: Discussionmentioning

confidence: 99%

Arthrocen, an avocado‐soy unsaponifiable agent, improves acetic acid‐induced colitis in rat by inhibition of NF‐kB signaling pathway

Goudarzi¹,

Partoazar

Mumtaz

et al. 2020

J Food Biochem

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The goal of the current study was to evaluate the anti‐inflammatory effect of Arthrocen against acetic acid‐induced colitis in rats. Acute inflammation was produced through intrarectal administration of 2 ml diluted acetic acid (4%) solution. All interventions were carried out for 5 days after colitis induction. Arthrocen was administered orally at doses of 30, 60, and 120 mg kg−1 day−1. Then, macroscopic and microscopic studies were performed. Myeloperoxidase (MPO) activity and tumor necrosis factor‐α (TNF‐α) activity were measured by biochemical and ELISA methods, respectively. Immunohistochemistry was done to investigate the expression of pNF‐κB. The results of this study demonstrated that Arthrocen reduced macroscopic and microscopic damage compared to the acetic acid group. Furthermore, Arthrocen decreased the activity of MPO and TNF‐α as well as the protein expression of pNF‐kB in rat colon tissue. The results of the current study revealed the anti‐inflammatory activity of Arthrocen in acetic acid mediated colon inflammation through suppressing the NF‐κB pathway. Practical applications Inflammatory bowel disease (IBD) is an immune‐mediated chronic relapsing disorder affecting the gastrointestinal tract (GIT) characterized by chronic bowel inflammation. A plant‐based dietary supplement containing avocado and soy unsaponifiable extracts in a ratio of 1:2 is known as Arthrocen. Arthrocen can be used as a complementary drug beside current drugs in clinical trials for the treatment of IBD.

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Avocado soybean unsaponifiables ameliorates cartilage and subchondral bone degeneration in mono-iodoacetate-induced knee osteoarthritis in rats

Cited by 23 publications

References 36 publications

Protective effect of ethyl acetate fraction from Semen sojae germinatum, the processed sprout of Chinese black soybean, on rat experimental osteoarthritis

Protective effect of ethyl acetate fraction from Semen sojae germinatum, the processed sprout of Chinese black soybean, on rat experimental osteoarthritis

Andrographis paniculata Extract Relieves Pain and Inflammation in Monosodium Iodoacetate-Induced Osteoarthritis and Acetic Acid-Induced Writhing in Animal Models

Arthrocen, an avocado‐soy unsaponifiable agent, improves acetic acid‐induced colitis in rat by inhibition of NF‐kB signaling pathway

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