Background. Individuals with Cluster C personality disorders (CPD) display hypersensitivity to threats and emotional dysregulation, it is therefore assumed that individuals with CPD employ dysfunctional stress regulation. However, the influence of personality features of CPD on the psychophysiological response to psychosocial stress has not been sufficiently explored. Therefore, this pilot study aimed to investigate how the maladaptive personality traits Detachment and Negative Affect (maladaptive traits characteristic of CPD in the DSM-5 alternative model for PD) account for variations in perceived stress, alexithymia, and in the emotional and physiological responses to stress.Methods. In the first study, 434 undergraduate students (363 low in Detachment/Negative Affect, 71 high in Detachment/Negative Affect) participated in a cross sectional questionnaire study, in which alexithymia (TAS-20) and perceived stress (PSS) were assessed and levels between groups were compared with an ANOVA. In the second study, we examined 52 low-trait and 24 high-trait individuals on heart rate, heart rate variability, skin conductance, and negative emotional state in response to and in recovery of the Trier Social Stress Test (TSST), with repeated measures ANOVAs.Results. Results showed that individuals with high-trait Detachment and Negative Affect report higher levels of alexithymia and perceived stress. In addition, high-trait individuals showed blunted heart rate reactivity to and recovery of stress, and an overall elevated negative emotional state during the TSST task, compared to the low-trait group. Conclusion. The results of the current study warrants the development and testing of interventions that target the emotional and physiological risk profile associated with high levels of trait Negative Affect and Detachment.