AVPR1A distribution in the whole C57BL/6J mouse neonate

Abstract

The neuropeptide arginine vasopressin (AVP) plays significant roles in maintaining homeostasis and regulating social behavior. In vaginally delivered neonates, a surge of AVP is released into the bloodstream at levels exceeding release during life-threatening conditions such as hemorrhagic shock. It is currently unknown where the potential sites of action are in the neonate for these robust levels of circulating AVP at birth. The purpose of this study is to identify the location of AVP receptor 1a (AVPR1A) sites as potential peripheral targets of AVP in the neonatal mouse. RT-qPCR analysis of a sampling of tissues from the head demonstrated the presence of Avpr1a mRNA, suggesting local peripheral translation. Using competitive autoradiography in wildtype (WT) and AVPR1A knockout (KO) postnatal day 0 (P0) male and female mice on a C57BL/6J background, specific AVPR1A ligand binding was observed in the neonatal mouse periphery in sensory tissues of the head (eyes, ears, various oronasal regions), bone, spinal cord, adrenal cortex, and the uro-anogenital region in the neonatal AVPR1A WT mouse, as it was significantly reduced or absent in the control samples (AVPR1A KO and competition). AVPR1A throughout the neonatal periphery suggest roles for AVP in modulating peripheral physiology and development of the neonate. Materials and methods Animals. Avpr1a mice 18 , fully backcrossed to C57BL/6J 19 , were bred in house. All breeding and experimental protocols were performed with the approval of Institutional Animal Care and Use Committee of Florida State University (protocols 1722 and 1746) in accordance with state and federal guidelines (Guide for the Care and Use of Laboratory Animals of the National Institutes of Health). Breeder pairs of heterozygous Avpr1a mice were housed in a temperature and humidity-controlled environment, on a 12 h light/dark cycle with food and

Oxytocin receptor disruption in Avil-expressing cells results in blunted sociability and increased inter-male aggression

“…This could function through known brain pathways or through peripheral sensory ganglia. AVP receptor 1a ( Avpr1a ) mRNA is present in sensory ganglia and receptor binding sites are present in various peripheral tissues of neonatal mice [ 25 , 26 ].…”