AW1 Neuronal Cell Cytotoxicity: The Mode of Action of Insecticidal Fatty Acids

Ren, Yuanhang; Shi, Jiaxing; Mu, Yangping; Tao, Ke; Jin, Hong; Hou, Taiping

doi:10.1021/acs.jafc.9b02197

Cited by 16 publications

(19 citation statements)

References 41 publications

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“…The amount of dead H. zea larvae at different concentrations (1000, 500, 250, 125, 62.5 mg L −1 ) of ICM was counted after 24, 48, 72, and 96 h to calculate the mortality rates. Mortality rate values were corrected with control mortality rates using Abbott's formula 9 and the median lethal dose (LD 50 ) values were calculate for the different periods.…”

Section: Methodsmentioning

confidence: 99%

“…The AW1 cell line was originally established from the ventral nerve cord of H. zea and their cultures were prepared according to a previously reported method 9,10 …”

Section: Methodsmentioning

confidence: 99%

“…Cytotoxicity of ICM on AW1 cells was evaluated with MTT assay using operation methods described in previous reports 9,10 . The incubation cells were exposed to ICM at various concentrations (6.25, 12.5, 25, 50 and 100 μmol L ‐1 ) for various lengths of time (24, 48 and 72 h).…”

Section: Methodsmentioning

confidence: 99%

“…Insecticidal activities of ICM against H. zea larvae (obtained from experimental base in Jingtang) were evaluated via the insect-dip method according to a previous study. 9 The amount of dead H. zea larvae at different concentrations (1000, 500, 250, 125, 62.5 mg L −1 ) of ICM was counted after 24, 48, 72, and 96 h to calculate the mortality rates. Mortality rate values were corrected with control mortality rates using Abbott's formula 9 and the median lethal dose (LD 50 ) values were calculate for the different periods.…”

Section: Insecticidal Activitiesmentioning

confidence: 99%

“…The AW1 cell line was originally established from the ventral nerve cord of H. zea and their cultures were prepared according to a previously reported method. 9,10 2.4 Cytotoxicity measurement Cytotoxicity of ICM on AW1 cells was evaluated with MTT assay using operation methods described in previous reports. 9,10 The incubation cells were exposed to ICM at various concentrations (6.25, 12.5, 25, 50 and 100 μmol L -1 ) for various lengths of time (24, 48 and 72 h).…”

Section: Cell Culturementioning

confidence: 99%

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Isochamaejasmin induces toxic effects onHelicoverpa zeaviaDNAdamage and mitochondria‐associated apoptosis

Ren

et al. 2020

Pest Management Science

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BACKGROUND: Stellera chamaejasme L. is a poisonous plant with rich resources and is thus highly valuable in terms of new pesticide development. Isochamaejasmin (ICM), one of the main ingredients in S. chamaejasme has drawn much attention owing to its antitumour properties. However, the toxicity and mode of action of ICM on insects are still not clear. In this article, the larva and neuronal cell (AW1) of Helicoverpa zea were used to clarify the insecticidal activity of ICM as well as its toxic mechanism at the cellular level. RESULTS: The results confirmed that ICM has potential toxicity against H. zea both in vivo and in vitro via time-and dosedependent manners. Moreover, we found that ICM caused DNA damage and increased the levels of γH2AX and OGG1 in AW1 cells. Results also showed decline in the mitochondrial membrane potential (MMP), upregulation of Bax/Bcl-2 expression resulting in the release of cytochrome c into the cytosol, activation of caspase-3/9, and cleavage of poly ADP-ribose polymerase (PARP) as a result of exposure to ICM. Additionally, a dose-dependent rise in the reactive oxygen species (ROS) levels, accumulation of a lipid peroxidation product, and inactivation of antioxidant enzymes were found in ICM-treated cells. CONCLUSION: These findings confirmed the insecticidal activity of ICM. Furthermore, the results revealed that ICM could cause DNA damage and induce apoptosis via the mitochondrial pathway in AW1 cells. This study provides the basic information needed to understand the toxicity and mechanisms of action of ICM, which could potentially be used to develop it as a new insecticide.

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Section: Methodsmentioning

confidence: 99%

“…The AW1 cell line was originally established from the ventral nerve cord of H. zea and their cultures were prepared according to a previously reported method 9,10 …”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Insecticidal Activitiesmentioning

confidence: 99%

Section: Cell Culturementioning

confidence: 99%

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Isochamaejasmin induces toxic effects onHelicoverpa zeaviaDNAdamage and mitochondria‐associated apoptosis

Ren

et al. 2020

Pest Management Science

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Neochamaejasmin B extracted from Stellera chamaejasme L. induces apoptosis through caspase‐10‐dependent way in insect neuronal cells

Jiang

et al. 2022

Arch Insect Biochem Physiol

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To explore the toxicity mechanisms of neochamaejasmin B (NCB) extracted from Stellera chamaejasme L., we first evaluated its cytotoxicity in neuronal cells of Helicoverpa zea (AW1 cells). NCB inhibited cell growth and was cytotoxic to AW1 cells in a dose-dependent manner.Further, transmission electron microscopy (TEM) was used to analyze the microstructure, and typical apoptotic characteristics were observed in AW1 cells treated with NCB. Moreover, the NCB-induced apoptosis was dose dependent. Subsequently, we explored the mechanism of apoptosis. A decline in the mitochondrial membrane potential (MMP) was found. Also, the levels of Bax were increased with increases in drug concentration, but there was no statistical difference in Bcl-2 levels at different NCB doses. Caspase-3 and caspase-10 activity was increased.These findings confirmed that NCB induced apoptosis in AW1 cells through a caspase-10-dependent mechanism.The results provide the basic information needed for understanding the toxicity and mechanisms of action of NCB, which could potentially be used to develop NCB as a new insecticide.

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A push–pull strategy to suppress stable fly (Diptera: Muscidae) attacks on pasture cattle via a coconut oil fatty acid repellent formulation and traps with m‐cresol lures

Lehmann

Brewer

Boxler³

et al. 2023

Pest Management Science

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BACKGROUND Stable flies [Stomoxys calcitrans (L.)] are economically important pests of cattle and other livestock. As an alternative to conventional insecticides, we tested a push–pull management strategy using a coconut oil fatty acid repellent formulation and an attractant‐added stable fly trap. RESULTS In our field trials we found that weekly applications of a push–pull strategy can reduce stable fly populations on cattle as well as a standard insecticide (permethrin). We also found that the efficacy periods of the push–pull and permethrin treatments following on‐animal application were equivalent. Traps with an attractant lure used as the pull component of the push–pull strategy captured sufficient numbers of stable flies to reduce on‐animal numbers by an estimated 17–21%. CONCLUSIONS This is the first proof‐of‐concept field trial demonstrating the effectiveness of a push–pull strategy using a coconut oil fatty acid‐based repellent formulation and traps with an attractant lure to manage stable flies on pasture cattle. Also notable is that the push–pull strategy had an efficacy period equivalent to that of a standard, conventional insecticide under field conditions. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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AW1 Neuronal Cell Cytotoxicity: The Mode of Action of Insecticidal Fatty Acids

Cited by 16 publications

References 41 publications

Isochamaejasmin induces toxic effects onHelicoverpa zeaviaDNAdamage and mitochondria‐associated apoptosis

Isochamaejasmin induces toxic effects onHelicoverpa zeaviaDNAdamage and mitochondria‐associated apoptosis

Neochamaejasmin B extracted from Stellera chamaejasme L. induces apoptosis through caspase‐10‐dependent way in insect neuronal cells

A push–pull strategy to suppress stable fly (Diptera: Muscidae) attacks on pasture cattle via a coconut oil fatty acid repellent formulation and traps with m‐cresol lures

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