Axenic culture of African trypanosome bloodstream forms

Hirumi, H.; Hirumi, K.

doi:10.1016/0169-4758(94)90402-2

Cited by 157 publications

(107 citation statements)

References 11 publications

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“…brucei Cell Culture-T. brucei brucei strain Lister 427 and the derived strains that constitutively expresses T7 polymerase and tetracycline (Tet) repressor protein (bloodstream form "single marker" and procyclic form 29-13) were used for cellbased experiments (10). BSFs were cultured in HMI-11 medium supplemented with 10% fetal bovine serum (FBS) (Tetfree, heat-inactivated; Atlanta Biologicals) at 37°C with 5% CO 2 (33). PFs were cultured in SDM-79 medium (Sigma) supplemented with 15% FBS at 28°C.…”

Section: Methodsmentioning

confidence: 99%

Deoxyhypusine Modification of Eukaryotic Translation Initiation Factor 5A (eIF5A) Is Essential for Trypanosoma brucei Growth and for Expression of Polyprolyl-containing Proteins

Nguyen

Leija

Kinch

et al. 2015

Journal of Biological Chemistry

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Background: eIF5A facilitates translation of mRNAs encoding proteins with polyprolyl repeats. Results: The protozoan parasite Trypanosoma brucei contains polyprolyl tracts in 15% of its proteome, and both eIF5A and its post-translational modification with deoxyhypusine are essential. Conclusion: Loss of eIF5A leads to abnormal cell morphology, abnormal cell division, and decreased flagellar attachment. Significance: Inhibitors of hypusine biosynthesis would disrupt multiple essential cellular processes.

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Section: Methodsmentioning

confidence: 99%

Deoxyhypusine Modification of Eukaryotic Translation Initiation Factor 5A (eIF5A) Is Essential for Trypanosoma brucei Growth and for Expression of Polyprolyl-containing Proteins

Nguyen

Leija

Kinch

et al. 2015

Journal of Biological Chemistry

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“…Trypanosoma brucei brucei (strain 427) bloodstream forms were cultivated in vitro in a modified Iscove's medium containing 10% heat-inactivated fetal calf serum (HI-FCS) and bloodstream form supporting factors: 0.05 mM bathocuproine sulfonate, 1.5 mM l-cysteine, 1 mM hypoxanthine, 0.2 mM 2-mercaptoethanol, 1 mM sodium pyruvate, 0.16 mM thymidine known as HMI 9 (Hirumi and Hirumi, 1994).…”

Section: Parasites Cell Lines and Mediamentioning

confidence: 99%

In vitro antitrypanosomal activity of ethnopharmacologically selected Beninese plants

Hoet

Opperdoes

Brun

et al. 2004

Journal of Ethnopharmacology

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The in vitro antitrypanosomal activity of methylene chloride, methanol and aqueous extracts of the leaves and twigs of five plant species traditionally used in Benin for the treatment of sleeping sickness were evaluated on Trypanosoma brucei brucei and their selectivity was analysed on Leishmania mexicana mexicana and J774 macrophage-like murine cells. The results showed that the four most active extracts had MIC values ≤19 g/ml (Hymenocardia acida twig and leaf, Strychnos spinosa leaf, Trichilia emetica leaf methylene chloride extracts).All these extracts had a lower activity on L. m. mexicana and J774 cells. Determination of the IC 50 values of the methylene chloride leaf extracts on two strains of trypanosomes (T. b. brucei and T. b. rhodesiense) and two mammalian cell lines (L6 and J774 cells) showed that all extracts possessed some antitrypanosomal activity with IC 50 's ranging from 1.5 to 39 g/ml. All were also toxic to the mammalian cells, but usually with higher IC 50 's. The only exception was the S. spinosa methylene chloride leaf extract which had no toxicity on J774 cells. Although tannins have been identified in most of the species studied, they could not be detected in the most active extracts, just as alkaloids. The presence of flavonoids and quinones may at least in part explain the observed activities of some of the active extracts.

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“…Bloodstream trypanosomes, strain Lister 427, were cultivated in HMI9 medium (41) at 37°C, 5% CO 2 , with 15% FBS and 2.5 g/ml G418 (39). The mRNA target regions for RNAi were selected using Trypano-FAN: RNAit Web-based service.…”

Section: Methodsmentioning

confidence: 99%

The Importance of the 45 S Ribosomal Small Subunit-related Complex for Mitochondrial Translation in Trypanosoma brucei

Ridlon

Škodová

Pan

et al. 2013

Journal of Biological Chemistry

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Background: Trypanosome mitochondria contain a 45 S ribosomal SSU-related complex with no precedence in other systems. Results: A selective ablation of the 45 S complex was associated with synthesis inhibition of some mitochondrial polypeptides. Conclusion:The 45 S complex is a factor required for mitochondrial translation that may have selective effects on different mRNAs. Significance: The mechanisms of mitochondrial translation in trypanosomes have no counterparts in other eukaryotes.

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Axenic culture of African trypanosome bloodstream forms

Cited by 157 publications

References 11 publications

Deoxyhypusine Modification of Eukaryotic Translation Initiation Factor 5A (eIF5A) Is Essential for Trypanosoma brucei Growth and for Expression of Polyprolyl-containing Proteins

Deoxyhypusine Modification of Eukaryotic Translation Initiation Factor 5A (eIF5A) Is Essential for Trypanosoma brucei Growth and for Expression of Polyprolyl-containing Proteins

In vitro antitrypanosomal activity of ethnopharmacologically selected Beninese plants

The Importance of the 45 S Ribosomal Small Subunit-related Complex for Mitochondrial Translation in Trypanosoma brucei

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