Axial structures control laterality in the distribution pattern of endothelial cells

Klessinger, Stephan; Christ, Bodo

doi:10.1007/bf00186689

Cited by 59 publications

(26 citation statements)

References 77 publications

(69 reference statements)

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“…What is the source of angioblasts that colonize the PNVP? We and others have shown that somite-derived angioblasts reproducibly contribute to PNVP formation (Wilting et al, 1995;Klessinger and Christ, 1996;Pardanaud et al, 1996;Pardanaud and Dieterlen-Lievre, 1999;Ambler et al, 2001), and in this study the neural tube was sufficient for formation of a somite-derived vascular plexus in collagen co-cultures. Presomitic mesoderm grafts placed next to a 'buffer' of avian cells that prevented direct contact with neural tube contributed to the PNVP, showing that progenitor/endothelial cells can migrate over a distance in response to neural tube vascular patterning signal(s).…”

Section: The Neural Tube As a Vascular Signaling Centersupporting

confidence: 66%

“…Although the invasion of angiogenic sprouts into neural tissue has been described, the developmental processes that pattern the PNVP have not been investigated. Both quailchick and mouse-quail chimera analysis identified somitederived precursor cells as an important source of endothelial cells that comprise the PNVP (Wilting et al, 1995;Klessinger and Christ, 1996;Pardanaud et al, 1996;Pardanaud and Dieterlen-Lievre, 1999;Ambler et al, 2001). Moreover, our recent analysis of ES cell-derived grafts showed that VEGF signaling is involved in vascular patterning around the midline of higher vertebrates (Ambler et al, 2003).…”

Section: Introductionmentioning

confidence: 96%

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The neural tube patterns vessels developmentally using the VEGF signaling pathway

et al. 2004

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Embryonic blood vessels form in a reproducible pattern that interfaces with other embryonic structures and tissues, but the sources and identities of signals that pattern vessels are not well characterized. We hypothesized that the neural tube provides vascular patterning signal(s) that direct formation of the perineural vascular plexus (PNVP) that encompasses the neural tube at mid-gestation. Both surgically placed ectopic neural tubes and ectopic neural tubes engineered genetically were able to recruit a vascular plexus, showing that the neural tube is the source of a vascular patterning signal. In mouse-quail chimeras with the graft separated from the neural tube by a buffer of host cells, graft-derived vascular cells contributed to the PNVP,indicating that the neural tube signal(s) can act at a distance. Murine neural tube vascular endothelial growth factor A (VEGFA) expression was temporally and spatially correlated with PNVP formation, suggesting it is a component of the neural tube signal. A collagen explant model was developed in which presomitic mesoderm explants formed a vascular plexus in the presence of added VEGFA. Co-cultures between presomitic mesoderm and neural tube also supported vascular plexus formation, indicating that the neural tube could replace the requirement for VEGFA. Moreover, a combination of pharmacological and genetic perturbations showed that VEGFA signaling through FLK1 is a required component of the neural tube vascular patterning signal. Thus, the neural tube is the first structure identified as a midline signaling center for embryonic vascular pattern formation in higher vertebrates, and VEGFA is a necessary component of the neural tube vascular patterning signal. These data suggest a model whereby embryonic structures with little or no capacity for angioblast generation act as a nexus for vessel patterning.

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Section: The Neural Tube As a Vascular Signaling Centersupporting

confidence: 66%

Section: Introductionmentioning

confidence: 96%

The neural tube patterns vessels developmentally using the VEGF signaling pathway

et al. 2004

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“…In addition, chimera studies have confirmed the migratory potential of PECs. In a number of avian experiments, quailderived graft cells were found to disperse as far as 400 µm (Klessinger and Christ, 1996), in cranial, caudal, lateral and medial directions within their host environment (Noden, 1989;Noden, 1990;Pardanaud and Dieterlen-Lievre, 1999;Poole and Coffin, 1989;Wilting et al, 1995;Wilting and Christ, 1996). Similar results were obtained with mouse-quail chimeras (Ambler et al, 2001).…”

Section: Discussionmentioning

confidence: 61%

αvβ3 integrin-dependent endothelial cell dynamics in vivo

2004

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A major challenge confronting developmental cell biologists is to understand how individual cell behaviors lead to global tissue organization. Taking advantage of an endothelial cell-specific marker and scanning time-lapse microscopy, we have examined the formation of the primary vascular pattern during avian vasculogenesis. Five types of distinguishable endothelial cell motion are observed during formation of a vascular plexus: (1) global tissue deformations that passively convect endothelial cells; (2) vascular drift, a sheet-like medial translocation of the entire vascular plexus; (3)structural rearrangements, such as vascular fusion; (4) individual cell migration along existing endothelial structures; and (5) cell process extension into avascular areas, resulting in new links within the plexus. The last four types of motion are quantified and found to be reduced in the presence of an αvβ3 integrin inhibitor. These dynamic cell motility data result in new hypotheses regarding primordial endothelial cell behavior during embryonic vasculogenesis.

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“…QH1 + ECs never crossed the dorsal midline (cf. Klessinger and Christ, 1996;Pardanaud et al, 1996). This aorta-associated QH1 pattern persisted until aortic hematopoiesis initiated, i.e.…”

Section: Unilateral Graftsmentioning

confidence: 99%

Somite-derived cells replace ventral aortic hemangioblasts and provide aortic smooth muscle cells of the trunk

et al. 2006

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We have previously shown that endothelial cells of the aortic floor give rise to hematopoietic cells, revealing the existence of an aortic hemangioblast. It has been proposed that the restriction of hematopoiesis to the aortic floor is based on the existence of two different and complementary endothelial lineages that form the vessel: one originating from the somite would contribute to the roof and sides, another from the splanchnopleura would contribute to the floor. Using quail/chick orthotopic transplantations of paraxial mesoderm, we have traced the distribution of somite-derived endothelial cells during aortic hematopoiesis. We show that the aortic endothelium undergoes two successive waves of remodeling by somitic cells: one when the aortae are still paired, during which the initial roof and sides of the vessels are renewed; and a second, associated to aortic hematopoiesis, in which the hemogenic floor is replaced by somite endothelial cells. This floor thus appears as a temporary structure, spent out and replaced. In addition, the somite contributes to smooth muscle cells of the aorta. In vivo lineage tracing experiments with non-replicative retroviral vectors showed that endothelial cells do not give rise to smooth muscle cells. However, in vitro, purified endothelial cells acquire smooth muscle cells characteristics. Taken together, these data point to the crucial role of the somite in shaping the aorta and also give an explanation for the short life of aortic hematopoiesis.

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Axial structures control laterality in the distribution pattern of endothelial cells

Cited by 59 publications

References 77 publications

The neural tube patterns vessels developmentally using the VEGF signaling pathway

The neural tube patterns vessels developmentally using the VEGF signaling pathway

αvβ3 integrin-dependent endothelial cell dynamics in vivo

Somite-derived cells replace ventral aortic hemangioblasts and provide aortic smooth muscle cells of the trunk

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