Ayahuasca blocks the reinstatement of methylphenidate-induced conditioned place preference in mice: behavioral and brain Fos expression evaluations

Reis, Henrique Sousa; Rodrigues, Isa R. S.; Anjos-Santos, Alexia; Libarino-Santos, Matheus; Serra, Yasmim A.; Cata-Preta, Elisângela G.; Oliveira-Campos, Daniella; Kisaki, Natali D.; Barros-Santos, Thaísa; Yokoyama, Thaís S.; Cruz, Fábio C.; Oliveira-Lima, Alexandre J.; Barbosa, Paulo César Ribeiro; Berro, Laís F.; Marinho, E.A.V.

doi:10.1007/s00213-020-05609-6

Cited by 14 publications

(6 citation statements)

References 68 publications

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“…Total distance traveled in the CPP apparatus also was measured during tests. The CPP design including a treatment phase, post-treatment test, alcohol re-exposure and post-re-exposure (reinstatement) test has been used in our laboratory for several years, with reliable results ( Silva et al, 2017 ; Cata-Preta et al, 2018 ; Reis et al, 2020 ; Libarino-Santos et al, 2020 ; Libarino-Santos et al, 2021 ). This protocol (including the choice of ethanol dose) has been used in our laboratory for several years, and has been shown to reliably induce CPP in mice, allow enough time for extinction and be sensitive to reinstatement upon an ethanol re-exposure, while allowing for treatment manipulations during extinction.…”

Section: Methodsmentioning

confidence: 99%

“…The present study aimed to investigate the effects of repeated IBO treatment on the reinstatement of ethanol-induced CPP under two conditions: 1) during a priming injection re-exposure test in which animals received a priming injection of ethanol and had free access to the CPP apparatus; 2) during a drug-free test conducted 24 h after a context-paired re-exposure, in which subjects received an injection of ethanol and were confined to the compartment previously conditioned to ethanol. Given our previous findings showing rewarding effects of another psychedelic substance ( Cata-Preta et al, 2018 ; Reis et al, 2020 ), we also investigated whether IBO induced CPP in mice.…”

Section: Introductionmentioning

confidence: 99%

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Ibogaine Blocks Cue- and Drug-Induced Reinstatement of Conditioned Place Preference to Ethanol in Male Mice

et al. 2021

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Ibogaine is a psychedelic extracted from the plant Tabernanthe iboga Baill. (Apocynaceae), natural from Africa, and has been proposed as a potential treatment for substance use disorders. In animal models, ibogaine reduces ethanol self-administration. However, no study to date has investigated the effects of ibogaine on ethanol-induced conditioned place preference (CPP). The present study aimed to investigate the effects of repeated treatment with ibogaine on the reinstatement of CPP to ethanol in male mice. The rewarding effects of ethanol (1.8 g/kg, i. p.) or ibogaine (10 or 30 mg/kg, p. o.) were investigated using the CPP model. Furthermore, we evaluated the effects of repeated treatment with ibogaine (10 or 30 mg/kg, p. o.) on the reinstatement of ethanol-induced CPP. Reinstatement was evaluated under two conditions: 1) during a priming injection re-exposure test in which animals received a priming injection of ethanol and had free access to the CPP apparatus; 2) during a drug-free test conducted 24 h after a context-paired re-exposure, in which subjects received an injection of ethanol and were confined to the compartment previously conditioned to ethanol. Our results show that ethanol, but not ibogaine, induced CPP in mice. Treatment with ibogaine after conditioning with ethanol blocked the reinstatement of ethanol-induced CPP, both during a drug priming reinstatement test and during a drug-free test conducted after re-exposure to ethanol in the ethanol-paired compartment. Our findings add to the literature suggesting that psychedelics, in particular ibogaine, may have therapeutic properties for the treatment of alcohol use disorder at doses that do not have rewarding effects per se.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ibogaine Blocks Cue- and Drug-Induced Reinstatement of Conditioned Place Preference to Ethanol in Male Mice

et al. 2021

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“…Rodent studies have demonstrated that ayahuasca decreases ethanol‐induced C‐Fos expression in the medial orbital cortex, and blocks place preference and ethanol‐induced sensitization 232–235 . Repeated ayahuasca ingestion reduces amphetamine self‐administration and C‐Fos expression in drug‐seeking brain areas, as well as methylphenidate‐induced place preference 222,236 . Preclinical works also suggest therapeutic potential in the use of β‐carbolines, especially harmine, in substance misuse disorders 21,237 .…”

Section: Ayahuasca and Mental Health Outcomesmentioning

confidence: 99%

Ayahuasca: A review of historical, pharmacological, and therapeutic aspects

Ruffell,

Crosland‐Wood,

Palmer

et al. 2023

PCN Reports

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Ayahuasca is a psychedelic plant brew originating from the Amazon rainforest. It is formed from two basic components, the Banisteriopsis caapi vine and a plant containing the potent psychedelic dimethyltryptamine (DMT), usually Psychotria viridis. Here we review the history of ayahuasca and describe recent work on its pharmacology, phenomenological responses, and clinical applications. There has been a significant increase in interest in ayahuasca since the turn of the millennium. Anecdotal evidence varies significantly, ranging from evangelical accounts to horror stories involving physical and psychological harm. The effects of the brew on personality and mental health outcomes are discussed in this review. Furthermore, phenomenological analyses of the ayahuasca experience are explored. Ayahuasca is a promising psychedelic agent that warrants greater empirical attention regarding its basic neurochemical mechanisms of action and potential therapeutic application.

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“…Ayahuasca administration has been shown to reduce voluntary amphetamine consumption, amphetamine-induced locomotor activity and anxiety in rats (Godinho et al, 2017), and attenuated naloxone-induced withdrawal syndrome in morphine-dependent rats (Aricioglu-Kartal et al, 2003). The treatment with harmaline reduced self-administration of morphine and cocaine (Glick et al, 1994), as well as β-carbolines altered patterns of Fos expression in brain regions associated with addictive behavior (Daldegan-Bueno, Favaro, Morais, et al, 2022;Nolli et al, 2020;Reis et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Preclinical studies showed that ayahuasca inhibited alcoholinduced sensitization and reduced the alcohol withdrawal symptoms in mice (Almeida et al, 2021;Oliveira-Lima et al, 2015). It was also observed that ayahuasca inhibited ethanol-induced conditioned place preference (CPP) expression (Cata-Preta et al, 2018;Gianfratti et al, 2022) and blocked the reinstatement of methylphenidate CPP induced by priming-injection (Reis et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Effect of Ayahuasca on Experimental Models of Ethanol Addiction and Immunohistochemistry Analysis in Rodents

Distefano Wiltenburg,

Morales-Lima,

Sousa Santos

et al. 2024

Preprint

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Ayahuasca, a psychoactive brew originally used ritually and therapeutically by various traditional peoples in the Amazon, is receiving special attention for its potential therapeutic effects on addiction. This study investigates the effects of lyophilized ayahuasca in alcohol-induced animal model, examining behavioral, pharmacological, and neurobiological aspects. Phytochemical analysis confirmed the presence of β-carbolines and dimethyltryptamine, with all compound levels within literature ranges. Pharmacological screening revealed dose-dependent behavioral changes, with the highest dose inducing transient effects. Motor coordination deficits were observed at the highest dose, consistent with pharmacological screening. Pretreatment with ayahuasca prevented ethanol-induced conditioned place preference, suggesting anti-addictive potential. Immunoreactivity analysis revealed no significant changes in DeltaFosB and c-Fos expression in the nucleus accumbens core and shell subsections. This study suggests low acute toxicity of ayahuasca, motor coordination impairment at high doses, and potential anti-addictive effects in preventing ethanol-induced conditioned place preference. These findings contribute to the understanding of the pharmacological and behavioral effects of ayahuasca and highlight the need for further research and clinical studies to elucidate its therapeutic potential in substance use disorders.

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Ayahuasca blocks the reinstatement of methylphenidate-induced conditioned place preference in mice: behavioral and brain Fos expression evaluations

Cited by 14 publications

References 68 publications

Ibogaine Blocks Cue- and Drug-Induced Reinstatement of Conditioned Place Preference to Ethanol in Male Mice

Ibogaine Blocks Cue- and Drug-Induced Reinstatement of Conditioned Place Preference to Ethanol in Male Mice

Ayahuasca: A review of historical, pharmacological, and therapeutic aspects

Effect of Ayahuasca on Experimental Models of Ethanol Addiction and Immunohistochemistry Analysis in Rodents

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