Azelaic acid and guanosine in tears improve discrimination of proliferative from non-proliferative diabetic retinopathy in type-2 diabetes patients: A tear metabolomics study

Wen, Xin; Ng, Tsz Kin; Liu, Qingping; Wu, Zhichong; Zhang, Mingzhi

doi:10.1016/j.heliyon.2023.e16109

Cited by 4 publications

(4 citation statements)

References 46 publications

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“…Another study showed that elevated expression of EV-miR-15a in plasma is correlated with initial retinal damage, suggesting its potential application in the early prevention of DR in patients with T2DM 87 . Similarly, miR-3976, miR-431-5p, and miR-26b-5p derived from serum EVs have been reported to be up-regulated in patients with DR 67 , 88 , 89 , indicating their potential utility in monitoring the progression of DR. Tears, a valid source of biomarkers for ocular diseases, show aberrant proteomics or metabolomics alterations in DR 90 , 91 . Moreover, EVs from tear samples have been isolated and performed transcriptomic analysis, which disclosed the upregulated miR-145-5p, miR-214-3p, and miR-9-5p and the downregulated miR-146a-5p, miR-31-5p, and miR-96-5p in diabetic patients with retinopathy compared with the control 92 .…”

Section: Evs In Dr Pathology and Diagnosismentioning

confidence: 99%

Emerging role of extracellular vesicles in diabetic retinopathy

Zhu,

Huang,

Sun

et al. 2024

Theranostics

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Diabetic retinopathy (DR), a complex complication of diabetes mellitus (DM), is a leading cause of adult blindness. Hyperglycemia triggers DR, resulting in microvascular damage, glial apoptosis, and neuronal degeneration. Inflammation and oxidative stress play crucial roles during this process. Current clinical treatments for DR primarily target the advanced retinal disorder but offer limited benefits with inevitable side effects. Extracellular vesicles (EVs) exhibit unique morphological features, contents, and biological properties and can be found in cell culture supernatants, various body fluids, and tissues. In DR, EVs with specific cargo composition would induce the reaction of receptor cell once internalized, mediating cellular communication and disease progression. Increasing evidence indicates that monitoring changes in EV quantity and content in DR can aid in disease diagnosis and prognosis. Furthermore, extensive research is investigating the potential of these nanoparticles as effective therapeutic agents in preclinical models of DR. This review explores the current understanding of the pathological effects of EVs in DR development, discusses their potential as biomarkers and therapeutic strategies, and paves the way for further research and therapeutic advancements.

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Section: Evs In Dr Pathology and Diagnosismentioning

confidence: 99%

Emerging role of extracellular vesicles in diabetic retinopathy

Zhu,

Huang,

Sun

et al. 2024

Theranostics

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“…The investigation of biomarkers from the AH and VH provides a deeper understanding of ocular diseases, including Glaucoma [ 50 ], Congenital Ectopia Lentis [ 51 ], and Diabetic Retinopathy [ 52 , 53 ]. Nonetheless, tears have been the non-invasive biological specimen of choice to find potential biomarkers in systemic diseases such as Type 2 diabetes mellitus (T2DM) [ 36 , 54 ], and ocular disorders (Keratoconjunctivitis [ 55 ] and Keratoconus [ 56 ]).…”

Section: Uncommon Biological Specimens In Clinical Metabolomicsmentioning

confidence: 99%

Critical Factors in Sample Collection and Preparation for Clinical Metabolomics of Underexplored Biological Specimens

de Souza,

Pereira,

de Sá

et al. 2024

Metabolites

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This review article compiles critical pre-analytical factors for sample collection and extraction of eight uncommon or underexplored biological specimens (human breast milk, ocular fluids, sebum, seminal plasma, sweat, hair, saliva, and cerebrospinal fluid) under the perspective of clinical metabolomics. These samples are interesting for metabolomics studies as they reflect the status of living organisms and can be applied for diagnostic purposes and biomarker discovery. Pre-collection and collection procedures are critical, requiring protocols to be standardized to avoid contamination and bias. Such procedures must consider cleaning the collection area, sample stimulation, diet, and food and drug intake, among other factors that impact the lack of homogeneity of the sample group. Precipitation of proteins and removal of salts and cell debris are the most used sample preparation procedures. This review intends to provide a global view of the practical aspects that most impact results, serving as a starting point for the designing of metabolomic experiments.

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“…The metabolites in tears could be potential biomarkers in DR analysis PDR group: Guanosine↑, Uric acid↑, D-(+)-malic acid↑, Pimelic acid↑, Azelaic acid↑, 2-Hydroxybenzothiazole↑, N, N-Diethyl-4-methoxybenzamide↓, Homovanillic acid↓, Phenol↓, Pipecolic acid↓, Guvacoline↓, Spironolactone↓, N,N-Diethyl-3-methoxybenzamide↓, Diazepam↓, Prostaglandin F2α 1-11-lactone↓, 2-Methoxyestrone↓, Tretinoin↓ [113] Diabetic retinopathy (DR)…”

Section: Ms-based Research In Gestational Diabetes Mellitusmentioning

confidence: 99%

“…Furthermore, retinopathy can serve as a biomarker for vascular disease risk in asymptomatic diabetic individuals, indicating an elevated risk of life-threatening systemic vascular complications [ 126 ]. In a recent study [ 113 ], GC-MS was employed to analyze tear fluid samples from 41 T2DM patients with DR as well as 21 non-diabetic patients. The study revealed a significant prominence of D-glutamate and its metabolism in the proliferative diabetic retinopathy (PDR) group compared to the non-diabetic group.…”

Section: Ms-based Metabolomics In Clinical Cases Of Diabetes-induced ...mentioning

confidence: 99%

Advanced Mass Spectrometry-Based Biomarker Identification for Metabolomics of Diabetes Mellitus and Its Complications

Zhang,

Shan,

et al. 2024

Molecules

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Over the years, there has been notable progress in understanding the pathogenesis and treatment modalities of diabetes and its complications, including the application of metabolomics in the study of diabetes, capturing attention from researchers worldwide. Advanced mass spectrometry, including gas chromatography–tandem mass spectrometry (GC-MS/MS), liquid chromatography–tandem mass spectrometry (LC-MS/MS), and ultra-performance liquid chromatography coupled to electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-ESI-Q-TOF-MS), etc., has significantly broadened the spectrum of detectable metabolites, even at lower concentrations. Advanced mass spectrometry has emerged as a powerful tool in diabetes research, particularly in the context of metabolomics. By leveraging the precision and sensitivity of advanced mass spectrometry techniques, researchers have unlocked a wealth of information within the metabolome. This technology has enabled the identification and quantification of potential biomarkers associated with diabetes and its complications, providing new ideas and methods for clinical diagnostics and metabolic studies. Moreover, it offers a less invasive, or even non-invasive, means of tracking disease progression, evaluating treatment efficacy, and understanding the underlying metabolic alterations in diabetes. This paper summarizes advanced mass spectrometry for the application of metabolomics in diabetes mellitus, gestational diabetes mellitus, diabetic peripheral neuropathy, diabetic retinopathy, diabetic nephropathy, diabetic encephalopathy, diabetic cardiomyopathy, and diabetic foot ulcers and organizes some of the potential biomarkers of the different complications with the aim of providing ideas and methods for subsequent in-depth metabolic research and searching for new ways of treating the disease.

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Azelaic acid and guanosine in tears improve discrimination of proliferative from non-proliferative diabetic retinopathy in type-2 diabetes patients: A tear metabolomics study

Cited by 4 publications

References 46 publications

Emerging role of extracellular vesicles in diabetic retinopathy

Emerging role of extracellular vesicles in diabetic retinopathy

Critical Factors in Sample Collection and Preparation for Clinical Metabolomics of Underexplored Biological Specimens

Advanced Mass Spectrometry-Based Biomarker Identification for Metabolomics of Diabetes Mellitus and Its Complications

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