Azo Pigments Make Raman Spectral Multiplexing More Sensitive

Wang, Shangfeng; Zhang, Fan

doi:10.1021/acscentsci.1c00527

Cited by 2 publications

(3 citation statements)

References 11 publications

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“…Utilizing various existing alkyne-based Raman tags, it is possible to achieve rapid, accurate, and real-time dynamic monitoring of biological macromolecules and various organelles in living cells. In addition to deuterium and alkynes, azo-enhanced Raman tags have also been extensively studied recently, and a series of azo tags with super sensitivity and fast multiplex imaging have been developed. ,− …”

Section: Materials Methodsmentioning

confidence: 99%

Recent Advances in Enhancement of Raman Scattering Intensity for Biological Applications

Tian

Zhang

Meng

et al. 2023

Chemical & Biomedical Imaging

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Raman scattering spectroscopy has attracted great interest for its significant potential in multiplexed biological imaging and sensing. However, the extremely weak molecular Raman scattering signal raises considerable concerns about its applicability in physiological conditions. Many multidisciplinary attempts, combining optics, materials science, and biology, have been conducted in the past decades to solve the problem of insufficient Raman scattering intensity and to realize the practical application of Raman scattering technology in the field of life science. In addition, to overcome the bottleneck of a single enhancement technology, more and more emphasis has been given to the combination of multiple Raman enhancement technologies. The underlying ideas behind each enhancement mechanism needs to be fully comprehended to effectively combine various techniques for desired signal intensity. Here, we summarize recent strategies developed for the enhancement of the signal strength of Raman scattering. We will also analyze the major advantages and disadvantages of each enhancement technique and provide perspectives for the compatibility and complementarity among different enhancement mechanisms.

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Section: Materials Methodsmentioning

confidence: 99%

Recent Advances in Enhancement of Raman Scattering Intensity for Biological Applications

Tian

Zhang

Meng

et al. 2023

Chemical & Biomedical Imaging

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“…This allows a typical spectral range to contain multiple different Raman bands for specific and reliable sensing (Figure b). − In the case of protonation/deprotonation reactions, the probe’s certain bonds may be broken, and new specific bonds are formed at the same time. , Based on the changes of the corresponding vibrational modes, fingerprint analysis of the multiple Raman bands allows for the subcellular pH quantification. However, Raman scattering faces the well-known challenge of low sensitivity. − To solve the challenge, we previously developed azo-enhanced Raman scattering (AERS), a novel approach that enables sensitive hyperspectral imaging by designing molecular structures with inherent intense Raman signals. , Based on the AERS method, the enhancing factors achieved 10 2 ∼10 4 × for certain Raman bands of molecular vibrations at the silent and fingerprint frequency regions.…”

Section: Introductionmentioning

confidence: 99%

“…37−41 To solve the challenge, we previously developed azo-enhanced Raman scattering (AERS), a novel approach that enables sensitive hyperspectral imaging by designing molecular structures with inherent intense Raman signals. 31,41 Based on the AERS method, the enhancing factors achieved 10 2 ∼10 4 × for certain Raman bands of molecular vibrations at the silent and fingerprint frequency regions. Inspired from the previous findings, we designed a smallmolecule lysosomal azo-based resonance Raman pH probe (LysoAzo-pH) for imaging proton distribution in cellular lysosomes (Figure 1c).…”

Section: ■ Introductionmentioning

confidence: 99%

Vibrational Fingerprint Analysis of an Azo-based Resonance Raman Scattering Probe for Imaging Proton Distribution in Cellular Lysosomes

et al. 2021

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Due to the fundamental mechanism of vibrational state transitions for chemical bonds, the spectra of Raman scattering are narrowbanded and photostable signals capable of probing specific reactions. In the case of protonation/deprotonation reactions, certain chemical bonds are broken and new bonds are formed. Based on the changes of the vibrational modes for the corresponding bonds, fingerprint analysis of multiple Raman bands may allow for the in situ visualization of proton distribution in live cells. However, Raman scattering faces the well-known challenge of low sensitivity. To perform the vibrational fingerprint analysis of Raman scattering by overcoming this challenge, we developed an azo-based resonance Raman pH probe. It was an azobenzene-featured small molecule responsive to protons with the inherent Raman signal ∼10 4 -fold more intense than that of the conventional alkyne-type Raman reporter 5-ethynyl-2′-deoxyuridine. Through the substitution of the electron-donating and -withdrawing entities to the azobenzene group, the effect of resonance Raman scattering and fluorescence quenching was obtained. This effect resulted in a significant Raman enhancement factor of ∼10 3 compared to the counterpart molecules without the molecular design. Based on the enhanced Raman sensitivity of the azo-based resonance Raman pH probe, the identification of vibrational fingerprint changes at the azo group was achieved during the protonation/deprotonation reactions, and the vibrational fingerprint analysis resolved a pH difference of less than 0.2 unit. The method enabled sensitive hyperspectral cell imaging that clearly visualized the change of proton distribution in autophagic cells.

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Azo Pigments Make Raman Spectral Multiplexing More Sensitive

Abstract: An azo-conjugated strategy to enhance Raman signals of vibrational bonds proves the giant potential of molecular engineering for ultrasensitive Raman multiplexed detection.

Cited by 2 publications

References 11 publications

Recent Advances in Enhancement of Raman Scattering Intensity for Biological Applications

Recent Advances in Enhancement of Raman Scattering Intensity for Biological Applications

Vibrational Fingerprint Analysis of an Azo-based Resonance Raman Scattering Probe for Imaging Proton Distribution in Cellular Lysosomes

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