Azole Antifungal Resistance in Candida albicans and Candida glabrata Isolated from Vulvovaginal Candidiasis Patients

Deravi, Niloofar; Fathi, Mobina; Tabatabaeifar, Seyede Nadia; Pooransari, Parichehr; Ahmadi, Bahram; Shokoohi, Gholamreza; Yaghoobpoor, Shirin; Vakili, Kimia; Lotfali, Ensieh; Ansari, Saham

doi:10.5812/archcid.106360

Cited by 6 publications

(2 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, resistance to Voriconazole and Itraconazole were observed in 8.4%, and 3.7% of stains. 26 The resistance rates of Fluconazole, Clotrimazole and Miconazole of the current study are higher than other population because of the irrational and excessive use of these drugs. A good agreement between DD method and BMD was reported in India which is similar to that found in this study.…”

Section: Discussionmentioning

confidence: 55%

Azoles resistance of candida species causing vulvo-vaginitis in reproductive age women at a tertiary care setting

et al. 2022

View full text Add to dashboard Cite

Objectives: To study prevalence and resistance pattern to azoles of candida species causing vulvovaginitis in reproductive age women. Methods: Samples were collected from Hayatabad Medical Complex from November 2018 to May 2019. This cross-sectional study was conducted at the department of Microbiology, Institute of Pathology and Diagnostic Medicine, Khyber Medical University Peshawar, Pakistan. A total of 369 high vaginal swabs were collected. Candida was isolated by vaginal swabs inoculation on Sabouraud’s dextrose agar (SDA). Colonies on SDA were inoculated on Candida CHROM agar to identify candida species. Wet film microscopy and Gram staining were performed. Biochemical identification was done with 20C AUX. Antifungal susceptibility testing was done by disc diffusion and broth micro-dilution methods to find the resistance pattern of azole drugs. Fluconazole, Clotrimazole, Miconazole, Voriconazole and Itraconazole were the azoles drugs used. Results: Among 43%(n=158) positive candida cases, 44%(n=85) were non pregnant women while 41%(n=73) were pregnant. The Candida species distribution of 158 isolates was as follow; Candida albicans (C. albicans) 46.2%(n=73), Candida krusei (C. krusei) 29.1%(n=46), Candida parapsilosis (C. parapsilosis) 19%(n=30) and Candida glabrata (C. glabrata) 5.7%(n=9). Overall Candida isolates were highly resistant 72%(n=113) to Fluconazole while least resistant 21.5 % (n=34) to Itraconazole. Conclusion: C. albicans is the most prevalent specie involved in Vulvovaginal candidiasis. Candida species were found to be least resistant to Itraconazole followed by Voriconazole, Miconazole, Clotrimazole and Fluconazole. doi: https://doi.org/10.12669/pjms.38.8.5984 How to cite this:Zaman R, Ullah I, Adeeb H, Arif A. Azoles resistance of candida species causing vulvo-vaginitis in reproductive age women at a tertiary care setting. Pak J Med Sci. 2022;38(8):---------. doi: https://doi.org/10.12669/pjms.38.8.5984 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

show abstract

Section: Discussionmentioning

confidence: 55%

Azoles resistance of candida species causing vulvo-vaginitis in reproductive age women at a tertiary care setting

et al. 2022

View full text Add to dashboard Cite

show abstract

“…All plates were left for 30 mints at room temperature; antifungal disks were placed on the surface of (MH-GMB) medium. The plates were incubated at 37°C for 24 h. The inhibition zone formed around the antifungal disks was measured in inhibition and calculated in (mm), and interpreted as described by CLSI (Sensitive S, resistant R, susceptible dose dependent (SDD) 13 .…”

Section: Antifungal Susceptibility Testing Of Candida Spp Isolatesmentioning

confidence: 99%

الكشف عن بعض عوامل الضراوة بين المبيضات المعزولة من المرضى وانتشار جين candidalysin ECE1

Al-Qaysi,

AL-Rubaie

2024

Baghdad Sci.J

View full text Add to dashboard Cite

Candida albicans is a common cause of respiratory infection and oral candidiasis in people; it is an opportunistic yeast pathogen and a major cause of morbidity and mortality in the immunocompromised persons and causes superficial infections of mucosal surfaces which affect millions of people throughout world. The main goal of this study was investigating the prevalence of some virulence factors which has the ability to configure biofilm formation, proteinase, hemolysin production among C.albicans isolates that include prevalence of candidalysin gene Eec1. Samples were collected during the period May and August of 2022 from 280 samples (swabs) of different ages and sexes of non-duplicated Iraqi patients suffering from oral candidiasis and respiratory diseases. The results showed that 102 were positive samples, 58(56.86%) from oral cavity and 44 (43.14%) from respiratory tract, while 178 of them were negative. Candida isolates were identified using conventional methods by grown on HiCrome Candida medium, germ tube production, chlamydospore formation and confirmed using VITEK-2 system, susceptibility of Candida isolates to antifungal drugs was examined by disk diffusion method, performed as recommended by (CLSI) M44-A document. The isolates showed a high level of susceptibility to Amphotericin-B (93.20%), Nystatin (90.20%), and Clotrimazole (85.92%). Prevalence of Candidalysin gene Eec1 among 70 isolates of C. albicans was investigated using polymerase chain reaction (PCR) technique, the results revealed that 41(58.57%) were harboring Ece1 gene for the oral cavity and respiratory tract. Only 34 (48.57%) C. albicans isolates were strong producer of biofilm, while 30 (42.86%) isolates produced proteinase, 20 (28.57%) of isolates had the ability to hemolyze the blood.

show abstract

In-vivo efficiency of the novel azole compounds (ATTAF-1 and ATTAF-2) against systemic candidiasis in a murine model

Fakhim,

Vaezi,

Morovati

et al. 2023

Journal of Medical Mycology

View full text Add to dashboard Cite

Azole Antifungal Resistance in Candida albicans and Candida glabrata Isolated from Vulvovaginal Candidiasis Patients

Cited by 6 publications

References 40 publications

Azoles resistance of candida species causing vulvo-vaginitis in reproductive age women at a tertiary care setting

Azoles resistance of candida species causing vulvo-vaginitis in reproductive age women at a tertiary care setting

الكشف عن بعض عوامل الضراوة بين المبيضات المعزولة من المرضى وانتشار جين candidalysin ECE1

In-vivo efficiency of the novel azole compounds (ATTAF-1 and ATTAF-2) against systemic candidiasis in a murine model

Contact Info

Product

Resources

About