Azospirilli of Uzbekistan Soils and their Influence on Growth and Development of Wheat Plants

Abstract

Associative nitrogen-fixing bacteria have been isolated, which were related to Azospirillum genus, by their morphological-cultural and physiological-biochemical ability to grow in microaerophilic conditions, as well as by a number of phenotypic traits. They comprised two species, namely, Azospirillum brasilense and Azospirillum lipoferum. Azospirilli strains displayed a varying salt resistance on potato medium containing a range of NaCl concentrations from 100 to 800 mM. The decrease in the nitrogen-fixing activity of azospirilli was detected starting from 200 mM NaCl. The biomass of the inoculated local varieties of wheat, Unumdor Bugdoi and Karlik 85, in microvegetation experiments exceeded the biomass of control plants by 20-50%. During the vegetation, some azospirilli strains formed spontaneous nodules on the wheat roots.

Study of root para-nodules formation in wheat (Triticum durum) inoculated with Frankia strain CcI3 and treated with 2, 4-dichlorophenoxyacetate (2, 4-D)

“…Para-nodule formation through 2,4-D treatment is a physiological process independent from bacterial action (Francisco and Akao, 1993). It is strongly possible that para-nodules obtained in our study without adding 2,4-D were resulting from the Frankia colonization, comparing to Saatovich (2006) who obtained para-nodules without the addition of phytohormones on the roots of wheat plants inoculated with Azospirillum. Biabani et al (2012) reported that the colonization of para-nodules is more extensive than that of lateral root emergence sites in plants which are not treated with 2,4-D as we found in the present study.…”

“…E-mail: aminadjemel@yahoo.fr. seeds were germinated in the dark under sterile conditions on moist filter paper in Petri dishes (Saatovich, 2006). The germinating seeds were then placed in 1 L bottles (sterilized by UV) containing sterile hydroponic solution and grown until one week old before inoculation with Frankia.…”

Functioning of Salt Tolerant Anabaena variabilis and Nostoc calcicola Strains in Salt Stress, Destructors of Hexachlorocyclohexane (HCH) in Saline Conditions

“…However, because we could recover live cells from 1 M NaCl medium we conclude that 1 M NaCl is not a lethal salinity for the strains but impairs growth and assumingly physiological characteristics. Other studies have shown that several salt tolerant Azotobacter and Azospirillum strains can survive up to 1.5 M NaCl not loosing their nitrogen fixing activity (Rasulov, 2010;Shakirov, 2006). At the same time at 0.8 M NaCl 75-80% acetylene-reductase activity, which is a proxy for nitrogen fixing activity, was preserved.…”

Quantitative comparison of ammonia and 3-indoleacetic acid production in halophilic, alkalophilic and haloalkalophilic bacterial isolates in soil

“…However, in some cases, the amount of osmotic regulator is inadequate. Thus, bacterial osmotic regulator can be used in plants (Rontein et al, 2002).Glycine betaine simulates plant growth and nitrogen fixation under the salinity stress condition In Azospirillum haloperfrans and Azospirillum brasilense (Hartman, 1988).These bacteria can improve the resistance of wheat to salinity via nitrogen fixation and hormones producing such as auxin (Saatovich, 2006).Usage of four genera of bacteria and two genera of actinomycete snonsymbiotic diazotrophs haloalkalophiles on wheat (Triticum aestivum L.) showed that plant dry weight was higher in the actinomycetes treatments than the bacteria genus. But the plants height in the bacteria treatments was higher (Butale et al, 2010).…”