Azospirillum brasilense FP2 modulates respiratory burst oxidase gene expression in maize seedlings

Espindula, Eliandro; Faleiro, Alexandro Cézar; Pereira, Tomás Pellizzaro; Amaral, Fernanda Plucani do; Arisi, Ana Carolina Maisonnave

doi:10.1007/s40502-017-0311-2

Cited by 4 publications

(6 citation statements)

References 27 publications

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“…The experiment was divided into four groups: Ctr (control plantlets), Yuc {plantlets that received 50 µM of yucasin solution [concentration according to Nishimura et al (2014 )]}, Azo (plantlets inoculated with A. brasilense FP2), and AzoYuc (plantlets that received 50 µM of yucasin solution and were inoculated with A. brasilense FP2). For inoculation, seeds (0.1 g) were mixed with 0.5 mL of bacterial suspension containing ~3 x 10 8 bacterial cells mL -1 ( Hungria et al, 2010 ; Espindula et al, 2017 ) and incubated for 5 min in an orbital shaker at 100 rpm ( Faleiro et al, 2013 ). Seeds inoculated or not were placed in a sterilized water-saturated paper and maintained for three days in a 25 o C growth chamber in the dark for germination.…”

Section: Methodsmentioning

confidence: 99%

Effects on gene expression during maize-Azospirillum interaction in the presence of a plant-specific inhibitor of indole-3-acetic acid production

Espindula,

Sperb,

Moz

et al. 2023

Genet. Mol. Biol.

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Amongst the sustainable alternatives to increase maize production is the use of plant growth-promoting bacteria (PGPB). Azospirillum brasilense is one of the most well-known PGPB being able to fix nitrogen and produce phytohormones, especially indole-3-acetic acid -IAA. This work investigated if there is any contribution of the bacterium to the plant's IAA levels, and how it affects the plant. To inhibit plant IAA production, yucasin, an inhibitor of the TAM/YUC pathway, was applied. Plantlets' IAA concentration was evaluated through HPLC and dual RNA-Seq was used to analyze gene expression. Statistical differences between the group treated with yucasin and the other groups showed that A. brasilense inoculation was able to prevent the phenotype caused by yucasin concerning the number of lateral roots. Genes involved in the auxin and ABA response pathways, auxin efflux transport, and the cell cycle were regulated by the presence of the bacterium, yucasin, or both. Genes involved in the response to biotic/ abiotic stress, plant disease resistance, and a D-type cellulose synthase changed their expression pattern among two sets of comparisons in which A. brasilense acted as treatment. The results suggest that A. brasilense interferes with the expression of many maize genes through an IAA-independent pathway.

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Section: Methodsmentioning

confidence: 99%

Effects on gene expression during maize-Azospirillum interaction in the presence of a plant-specific inhibitor of indole-3-acetic acid production

Espindula,

Sperb,

Moz

et al. 2023

Genet. Mol. Biol.

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“…Based on the relevance of RZ2MS9 to maize growth promotion and changes in root architecture and considering scarcity of studies addressing plant response to PGPR inoculation at transcriptional level (Plucani do Amaral et al, 2014;Gond et al, 2015;Ding et al, 2017;Espindula et al, 2017), we directed our study toward investigation of maize genes modulated by RZ2MS9 and phenotypic changes and their related with growth parameters both in vitro and under greenhouse conditions.…”

Section: Plant Inoculation With Beneficial Microorganisms For Instancmentioning

confidence: 99%

“…Major plant growth and development changes rely on the combined effect of cell division stimulated by cytokinin and elongation stimulated by auxin, the amount of each hormone present in the tissue in active form at a specific time (Taiz et al, 2017). However few studies evaluated host response to microbial inoculation at transcriptional level (Plucani do Amaral et al, 2014;Gond et al, 2015;Ding et al, 2017;Espindula et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

New insights into Plant Growth Promoting Rhizobacterium >i<Bacillus thuringiensis>/i< RZ2MS9 biology: entomopathogenic activity and molecular interaction with >i<Zea mays>/i< L.

Longatto¹

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pela oportunidade de realizar o curso de Doutorado. À minha orientadora profª Drª Maria Carolina Quecine Verdi pela inspiração, acolhimento, compreensão, pragmatismo, bons conselhos e, sobretudo, por acreditar em nosso trabalho, mesmo em meio às mudanças. Ao Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) processo 140590/2017 e ao Programa de Aperfeiçoamento do Ensino (PAE-USP) pelas bolsas concedidas. O presente trabalho foi realizado com apoio da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil (CAPES)-Código de Financiamento 001. Ao professor Dr. João Lucio de Azevedo pela agradável convivência, sabedoria, entusiasmo e bom humor. Ao professor Dr. Roberto Fristche Neto do Laboratório de Plantas Alógamas da ESALQ pela disponibilização das sementes de milho utilizadas nos experimentos de casa de vegetação e da estufa utilizada para secagem de amostras vegetais. À professora Dra. Claudia Barros Monteiro Vitorello pelo apoio e aconselhamento em momentos importantes e por ter disponibilizado equipamento para realização das análises de expressão gênica. Ao professor Dr. José Roberto Postali Parra e a querida técnica Sra Neide Graciano Zério e demais colegas do Laboratório de Biologia de Insetos da ESALQ por terem nos recebido tão bem e fornecido todo suporte para realização dos testes entomológicos. À professora Dra. Maria Lúcia Carneiro Vieira, do Laboratório de Biologia Celular e Molecular de Plantas da ESALQ, pela disponibilização das estufas utilizadas para experimento de casa de vegetação e para preparo de vidraria para extrações de RNA. Aos professores Dr. Fábio Tebaldi Nogueira, Dr. Marcio Castro Silva-Filho e minha orientadora, e colegas Carolina, Filipe, Gleicy, Paula, Renata e Raphael, pelos ensinamentos, conselhos, apoio e atitudes apreendidos durante os dois Estágios Supervisionados em Docência realizados na ESALQ. Ao professor Dr. Antonio Vargas de Oliveira Figueira e Dra. Flavia Bento pelo aprendizado, convivência, respeito e conselhos que levarei comigo da etapa vivida no CENA.

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“…Rice plants inoculated and then submitted to drought stress showed higher tolerance to stress, increased root growth and development, higher expression of the IAA gene, and higher concentration of IAA hormone [ 94 ]. Roots of rice inoculated with H. seropedicae showed that auxin-responsive genes were repressed, while maize inoculated with A. brasilense showed a significant increase in Auxin transporter-like protein 1 [ 93 , 100 ]. Remarkably, analysis of sugarcane genotypes with contrasting BNF efficiencies showed differences in auxin biosynthesis, auxin transport, and auxin signaling, as the auxin pathway was activated more in roots of the genotype that associated best with diazotrophic bacteria (i.e., high BNF) [ 79 ].…”

Section: Molecular Mechanisms Involved In the Association Of Plant An...mentioning

confidence: 99%

“…RNA-seq analysis of maize inoculated with A. brasilense sp245 or H. seropedicae HRC54 showed that DEGs involved in GA signal transduction and response were induced in plants inoculated with both bacteria [ 82 ]. In other studies, it has been observed that genes involved in gibberellin synthesis were induced in maize plants inoculated with A. brasilense or H. seropedicae [ 100 , 109 ]. These data suggest that GA might contribute to the regulation of developmental adaptations in plants in response to beneficial bacteria stimulus.…”

Section: Molecular Mechanisms Involved In the Association Of Plant An...mentioning

confidence: 99%

The Impact of Non-Nodulating Diazotrophic Bacteria in Agriculture: Understanding the Molecular Mechanisms That Benefit Crops

Thiebaut

Urquiaga

Rosman

et al. 2022

IJMS

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Agriculture is facing increasing challenges with regard to achieving sustainable growth in productivity without negatively impacting the environment. The use of bioinoculants is emerging as a sustainable solution for agriculture, especially bioinoculants based on diazotrophic bacteria. Brazil is at the forefront of studies intended to identify beneficial diazotrophic bacteria, as well as in the molecular characterization of this association on both the bacterial and plant sides. Here we highlight the main advances in molecular studies to understand the benefits brought to plants by diazotrophic bacteria. Different molecular pathways in plants are regulated both genetically and epigenetically, providing better plant performance. Among them, we discuss the involvement of genes related to nitrogen metabolism, cell wall formation, antioxidant metabolism, and regulation of phytohormones that can coordinate plant responses to environmental factors. Another important aspect in this regard is how the plant recognizes the microorganism as beneficial. A better understanding of plant–bacteria–environment interactions can assist in the future formulation of more efficient bioinoculants, which could in turn contribute to more sustainable agriculture practices.

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Azospirillum brasilense FP2 modulates respiratory burst oxidase gene expression in maize seedlings

Cited by 4 publications

References 27 publications

Effects on gene expression during maize-Azospirillum interaction in the presence of a plant-specific inhibitor of indole-3-acetic acid production

Effects on gene expression during maize-Azospirillum interaction in the presence of a plant-specific inhibitor of indole-3-acetic acid production

New insights into Plant Growth Promoting Rhizobacterium >i<Bacillus thuringiensis>/i< RZ2MS9 biology: entomopathogenic activity and molecular interaction with >i<Zea mays>/i< L.

The Impact of Non-Nodulating Diazotrophic Bacteria in Agriculture: Understanding the Molecular Mechanisms That Benefit Crops

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