Azospirillum sp. in current agriculture: From the laboratory to the field

Cassán, Fabricio; Díaz‐Zorita, Martín

doi:10.1016/j.soilbio.2016.08.020

Cited by 270 publications

(152 citation statements)

References 61 publications

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“…The potential of Pseudomonas AZM-01 and derivative EDA-resistant mutants to produce auxins was demonstrated in the presented study, and this trait should be related to the modifications on roots of plants inoculated with the wild and mutant strains. PGPB diazotrophic species of Azospirillum genus are the most studied and the most common bacteria in commercial inoculants (Cassán and Diaz-Zorita 2016). Ammonium-excreting mutants of A. brasilense improved the accumulation of biomass and N of wheat plants (Christiansen-Weniger and Van Veen 1991;Van Dommelen et al 2009).…”

Section: Discussionmentioning

confidence: 99%

“…Although the identification of diazotrophic bacteria in association with non-leguminous plants is common, the ability of these microorganisms to contribute effectively to nitrogen nutrition of the plant is still controversial (Santi et al 2013;Cassán and Diaz-Zorita 2016). While the transfer of N fixed by symbiotic bacteria to the legume plants is a well-documented event, the mechanisms for N transfer involving non-symbiotic bacteria and nonleguminous plants are poorly understood.…”

Section: Introductionmentioning

confidence: 99%

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Ammonium excretion, auxin production and effects of maize inoculation with ethylenediamine-resistant mutants of Pseudomonas sp.

et al. 2018

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Plant growth-promoting bacteria (PGPB) comprise part of plant microbiome of biotechnological interest due to their potential to decrease the use of agrochemicals in agriculture.Among the commonly found PGPB species, the Pseudomonas genus is known for high competitiveness and efficiency in expressing growth-promotion traits. To increase the contribution of diazotrophic Pseudomonas sp. to the plant nitrogen nutrition, the strain AZM-01 was chemically mutagenized with methyl methanesulfonate (MMS), following the selection for resistance to ethylenediamine (EDA). From the 13 EDA-resistant mutant strains selected, four showed increased the ammonium excretion, with the highest value reaching up to 284% increase as compared to the wild strain, and six strains were found to produce significantly more auxins than the wild strain. Two independent inoculation trials with the wild and

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ammonium excretion, auxin production and effects of maize inoculation with ethylenediamine-resistant mutants of Pseudomonas sp.

et al. 2018

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“…Liquid formulations should be considered in this aspect, because unlike solid formulations, they allow addition of sufficient amount of nutrient and cell protectants, improving bacteria shelf life (Brar et al, 2012). About 80% of biological products containing Azospirillum sp., a PGPR available for commercialization in South America, uses liquid carriers for the formulation of these biological products with more frequent shelf life of the registered products being 6 months (Cassan et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Shelf life enhancement of plant growth promoting rhizobacteria using a simple formulation screening method

Arriel-Elias¹,

Oliveira²,

Silva-Lobo³

et al. 2018

Afr. J. Microbiol. Res.

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Plant growth promoting rhizobacteria (PGPR) are a specific group of bacteria interacting beneficially with plants. Among the known PGPRs, the species Pseudomonas fluorescens and Burkholderia pyrrocinia have been highlighted in both growth promotion and control of rice diseases. Ensuring the stability of the microorganism during production, formulation, distribution and storage has been a challenge for these species. In this context, the objective of this work was to develop liquid formulations, through a simplified process, that allows increase in the shelf life of these rhizobacteria for commercial application. Both bacteria were tested in 32 formulations under two storage temperature conditions: 8 and 28°C, resulting in 64 treatments for each species, which were evaluated for 180 days. Combinations of the adjuvants: molasses, glycerol, NaCl, PVP, MgSO 4 , K 2 HPO 4 and yeast extract were evaluated. Formulations containing molasses, stored at 8°C, were considered the most efficient in maintaining microbial viability. The method used was considered efficient to select three formulations that allowed maintenance of the concentration of viable cells of P. fluorescens and B. pyrrocinia in 10 8 cfu.mL -1 , for at least 90 and 150 days, respectively, not interfering with bacterial action potential.

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“…Furthermore, many of these bacteria have additional mechanisms such as the synthesis of phytohormones, induction of plant-stress tolerance and defense genes, among others (Bashan and de-Bashan, 2010;Fukami et al, 2017a, b), which may help to promote plant growth. Due to their multiple beneficial mechanisms, these bacteria have been classified as plant-growth-promoting bacteria (PGPB), and among them, those belonging to the Azospirillum brasilense species are the most studied and employed as inoculants worldwide, with consistent responses to inoculation in all continents, highly contributing to the economy of chemical fertilizers (Okon and Labandera-Gonzalez, 1994;Dobbelaere et al, 2001;Hartmann and Bashan, 2009;Bashan and de-Bashan, 2010;Hungria et al, 2010;Okon et al, 2015;Cassán and Diaz-Zorita, 2016;Pereg et al, 2016;Fukami et al, 2016Fukami et al, , 2017a.…”

Section: Introductionmentioning

confidence: 99%

Production of polyhydroxybutyrate (PHB) and biofilm by Azospirillum brasilense aiming at the development of liquid inoculants with high performance

Santos¹,

Hungría²,

Nogueira³

2017

Afr. J. Biotechnol.

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Azospirillum brasilense strains Ab-V5 and Ab-V6 have been broadly and successfully used in commercial inoculants in Brazil, for both non-legumes and legumes, contributing to increases in grain yields with reduced applications of chemical fertilizers. Azospirillum survival, however, may be very low in liquid inoculant formulations and strategies such as the enrichment with polyhydroxybutyrate (PHB) and biofilm may help both bacterial survival and agronomic performance. The production was quantified for both PHB and biofilm by strains Ab-V5 and Ab-V6 in liquid inoculant formulations. Differences were observed between formulations, strains, and strain x formulation. Cellular PHB concentrations ranged from 7.9 to 40.2% of the cell dry weight after 96 h, and considerable amounts of biofilm were synthesized by both Ab-V5 and Ab-V6. Maximum accumulation of PHB and biofilm occurred with A. brasilense strain Ab-V6 in the formulation FORM2+P3, indicating that it is possible to enrich the inoculants on PHB and biofilm by improving the culture media. Field experiments will now be performed to confirm the agronomic efficiency of the improved inoculant.

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Azospirillum sp. in current agriculture: From the laboratory to the field

Cited by 270 publications

References 61 publications

Ammonium excretion, auxin production and effects of maize inoculation with ethylenediamine-resistant mutants of Pseudomonas sp.

Ammonium excretion, auxin production and effects of maize inoculation with ethylenediamine-resistant mutants of Pseudomonas sp.

Shelf life enhancement of plant growth promoting rhizobacteria using a simple formulation screening method

Production of polyhydroxybutyrate (PHB) and biofilm by Azospirillum brasilense aiming at the development of liquid inoculants with high performance

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