AZT on telomerase activity and cell proliferation in HS 839.T melanoma cells

Abstract

PURPOSE:To evaluate telomerase activity and proliferation of HS839.T melanoma cells, subjected to the action of AZT. METHODS:Cells were grown in triplicate, AZT at different concentrations: 50, 100 and 200μM, was added and left for 24 and 48 hours, and its effects were compared with the control group. Telomerase activity was detected by PCR and cell proliferation was evaluated by MTT. RESULTS:After 24 hours, there was no inhibition of cell proliferation or telomerase activity when compared to the control group. After 48 hours, there was a momentary decrease, suggesting that the cell lines used in this study are sensitive to AZT, but quickly recover both the enzyme activity and cell proliferation. CONCLUSION:The action of AZT on the melanoma cells studied, at the concentrations and times tested, did not inhibit telomerase activity nor affect cell proliferation.Key words: Telomerase. Melanoma. Zidovudine. Cell Proliferation. In Vitro.RESUMO OBJETIVO: Avaliar a atividade da telomerase e da proliferação de células de melanoma HS839.T submetidas à ação do AZT. MÉTODOS:As células foram cultivadas, em triplicata, com diferentes concentrações de AZT: 50, 100 e 200µM, por 24h e 48h, seus efeitos comparados com o grupo controle. A atividade da telomerase foi detectada por PCR e a proliferação celular avaliada por MTT. RESULTADOS:No tempo de 24 horas, não houve inibição da proliferação celular e da atividade da telomerase em comparação com o grupo controle. No período de 48 horas, houve uma diminuição momentânea, sugerindo que as células das linhagens utilizadas neste estudo são sensíveis ao AZT, mas que recuperam a atividade enzimática e proliferativa.CONCLUSÃO: Nas células de melanoma HS839.T estudadas e nas concentrações e tempos propostos, a ação do AZT não inibiu a atividade da telomerase e não afetou a proliferação celular.Descritores: Telomerase. Melanoma. Zidovudina. Proliferação de Células. In Vitro.Souza Sobrinho CP et al.

Differences in telomerase activity and the effects of AZT in aneuploid and euploid cells in colon cancer

“…Azido-deoxythymidine (AZT) is a type of nucleoside analogue that is approved by the Food and Drug Administration to treat acquired immune deficiency syndrome (AIDS). AZT inhibits DNA double-strand formation by destroying the RNA reverse transcriptional ability of the virus, which results in the loss of the replication template and a consequential decrease in the replication rate (28)(29)(30)(31). AZT has been shown to inhibit telomerase activity in a variety of cancer cells, including colon, human brain glioma and liver cancer cells (32)(33)(34).…”