Afferent lymph veiled cells stimulate proliferative responses in allogeneic CD4⁺ and CD8⁺ T cells but not γδ TCR⁺ T cells

Abstract

SUMMARYDendritic cells were identified in afferent lymph derived by lymphatic cannulation of cattle, stained with monoclonal antibody (mAb) to the bovine workshop cluster 6 (WC6) antigen, which is highly expressed on bovine afferent lymph veiled cells, and sorted with a fluorescence-activated cell sorter. These cells expressed major histocompatibility complex (MHC) class I and II and CD1b but not CD14.

Seroma fluid subsequent to axillary lymph node dissection for breast cancer derives from an accumulation of afferent lymph

“…On the other hand, also cellular analysis, represented almost exclusively by mononuclear cells, supports the hypothesis that seroma fluid is an accumulation of afferent lymph. Further supporting this hypothesis, the presence of large mononuclear cells only partially expressing CD14 is reminiscent of "veiled cells" detectable in afferent lymph [18]. Also, the finding of extremely high levels of IL-6 in seroma is strongly suggestive for an accumulation of afferent lymph.…”

Rapid and Transient Activation of γδ T Cells to IFN-γ Production, NK Cell-Like Killing, and Antigen Processing during Acute Virus Infection

“…The gd T cells may also be involved in Ag presentation independently from dendritic cells. In work performed by Howard et al (40) + cells were eliminated, the breadth of immune responses to virus often compensates for lacking a particular function. The Juleff study did not include multiple Ab treatments to address what happens when multiple responding cell populations are blocked/eliminated in the same animal, most likely due to the very high cost of such a study.…”

In Vitro Responsiveness of γδ T Cells fromMycobacterium bovis-Infected Cattle to Mycobacterial Antigens: Predominant Involvement of WC1+Cells

“…In the bovine system it has been reported that purified WC1 ϩ T cells require the addition of exogenous IL-2 for proliferation in response to Thileria annulata-infected autologous cells but that IL-2 alone induces a limited WC1 ϩ proliferative response (16). Other studies have also reported that IL-2 alone induces minimal proliferation of bovine WC1 ϩ cells, implying that IL-2 is required as a secondary signal for activation of ␥␦ cells (15,29), driving them to proliferation (16). In the present study, the high degree of proliferation of purified WC1 ϩ T cells in response to MBSE and CF was observed only in infected animals, indicating the specificity of the response to M. bovis antigens, with a possible requirement for IL-2 and/or other cytokines supplied by other cells.…”