B-cell differentiation following autologous, conventional, or T-cell depleted bone marrow transplantation: a recapitulation of normal B-cell ontogeny

Abstract

The circulating lymphocytes of 88 consecutive patients following autologous, conventional, or T-cell depleted bone marrow transplantation were serially analyzed for B-cell surface antigen expression and function. In the majority of patients, except for those who developed chronic graft-versus-host disease, the number of circulating CD20+ B cell normalized by the fourth posttransplant month. The earliest detectable B cells normally expressed HLA-DR, CD19, surface immunoglobulin (slg), CD21, Leu-8, and lacked expression of CD10 (CALLA). In addition, the circulating B cells expressed CD1c, CD38, CD5, and CD23 for the first year following transplant, antigens that are normally expressed on a small percentage of circulating B cells in normal adults, but highly expressed on cord blood B cells. Similar to cord blood B cells, patient B cells isolated during the first year following transplant, proliferated normally to Staphylococcus aureus Cowan strain I (SAC), and produced IgM, but minimal or no IgG when stimulated with pokeweed mitogen and SAC, unlike normal adult B cells that produce both. The similar phenotype and function of posttransplant and cord blood B cells, and their similar rate of decline in patients and normal children adds further evidence to support the hypothesis that B-cell differentiation posttransplant is recapitulating normal B-cell ontogeny.

In Vitro Culture During Retroviral Transduction Improves Thymic Repopulation and Output After Total Body Irradiation and Autologous Peripheral Blood Progenitor Cell Transplantation in Rhesus Macaques

“…1). For all groups, we observed supranormal absolute B‐cell numbers throughout the year post‐transplantation, as described earlier in clinical studies [19, 20]. There were no statistical significant differences in the reconstitution pattern between the groups after transplantation.…”

Humoral immune responses in humanized BLT mice immunized with West Nile virus and HIV-1 envelope proteins are largely mediated via human CD5+ B cells

“…It was previously reported that NOD/SCID mice engrafted with human cord‐blood‐derived HSC generate high levels of CD19 + CD5 + human B lymphocytes in the lymphoid tissues 14 . It is possible that the fetal progenitor cells (cord blood or fetal‐liver‐derived HSC) in comparison with their adult counterpart (mobilized peripheral blood or bone‐marrow‐derived HSC) has a higher potential to differentiate into CD5 + B cells; however, it has also been demonstrated that the lack of optimal T‐cell–B‐cell signalling may bias the B‐cell developmental pathway towards the production of this B‐cell subset 34 . As implantation of human fetal thymus in the BLT model resulted in apparently normal T‐cell development (determined by surface marker expression, see supplementary material, Fig.…”

Reconstituição imunológica após o transplante de medula óssea alogênico

“…Segundo Small et al (1990), 25 o número relativo e absoluto de células circulantes expressando CD19 e CD20, dois marcadores das células B maduras, aparece diminuído durante os três primeiros meses após o transplante. Além disso, durante o primeiro ano pós-transplante, a maioria das células B expressa marcadores de fenótipos indiferenciados, como CD23 e CD38.…”