B Cell Lymphoma-2-Modified Bone Marrow-Derived Mesenchymal Stem Cells Transplantation for the Treatment of Diabetes Mellitus-Induced Erectile Dysfunction in a Rat Model

Abstract

Objective: The study aimed to explore the effects of B cell lymphoma-2 (Bcl-2)-modified bone marrow-derived mesenchymal stem cells (BMSCs) transplantation for the treatment of diabetes mellitus-induced erectile dysfunction (DMED) in a rat model. Methods: The DMED rat model was successfully established. Thirty-six DMED rats were assigned into the Bcl-2-BMSCs, null-BMSCs, BMSCs and phosphate buffered saline (PBS) groups. Meanwhile, 9 normal rats injected with PBS were taken as the normal control group. Results: In the Bcl-2-BMSCs group, the average times of erection, rate of erection, peak intra-cavernous pressure (ICP) and peak ICP/mean arterial pressure were higher than those in the null-BMSCs, BMSCs and PBS groups, but were lower than those in the normal control group. In the Bcl-2-BMSCs group, capillary vessels and Bcl-2 mRNA and protein expressions were similar to those in the normal control group, while they were higher than those in other groups. Conclusion: These findings indicate that Bcl-2-modified BMSC transplantation could improve erectile function in DMED rats.

Extracellular Vesicles From Human Urine-Derived Stem Cells Ameliorate Erectile Dysfunction in a Diabetic Rat Model by Delivering Proangiogenic MicroRNA

“…Stem cell therapies represent a promising new frontier for the treatment of refractory DED. A growing body of research is demonstrating the therapeutic effects of numerous types of stem cells 6, 7, 8, 9, 10. We previously reported that adipose-derived stem cells may correct DED 11 and cavernous nerve injury–induced erectile dysfunction (CNIED) 12 .…”

Towards clinical application of tissue engineering for erectile penile regeneration

“…Several mammalian models are used to study tissue engineering in corpus cavernosum and tunica albuginea regeneration, most frequently rabbits, rats and mice [30][31][32][33][34][35][36][37][38][39] . Animal models are useful for penile regeneration studies; however, ethical concerns highlight that animal suffering should be replaced with in-vitro analysis and computational modelling when possible 40 .…”

“…MSCs are one of the main sources of autologous stem cells used in neocorporal tissue engineering, as they are relatively easy to isolate from various tissues, such as fat, and expand under standard culture conditions 33,63 .…”

Comparison of critical biomarkers in 2 erectile dysfunction models based on GEO and NOS-cGMP-PDE5 pathway

“…[ 4 ] At present, the most classical modeling method is the rat model of DMED, induced by streptozotocin. [ 5 ] The commonly used method to create CNIED model is to squeeze, freeze, or cut off unilateral or bilateral cavernous nerves. [ 6 ] Vascular and psychological ED animal models were also established according to different etiology.…”