B cells expressing cd5 are increased in sjögren's syndrome

Dauphinée, Michael J.; Tovar, Zair; Talal, Norman

doi:10.1002/art.1780310509

Cited by 211 publications

(89 citation statements)

References 24 publications

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“…The leukemic cells in CLL express the CD5 molecule and produce autoantibodies; the same subpopulation of B cells is found in increased numbers in peripheral blood and salivary gland samples from patients with primary SS (38-40). Interestingly, the percentage of these cells has been shown to be higher in primary SS patients with detectable monoclonal paraproteins than in those without (38), and remission of malignant lymphoma in some primary SS patients has been shown to be accompanied by restoration of CD5+ B cell levels to normal (39). Perhaps the lymphoproliferation in primary SS is highly selective and a distinct subpopulation of B cells, possibly those expressing the CD5 molecule, is selected and may be the same subpopulation of B cells involved in such other B cell neoplasms as Waldenstrom's macroglobulinemia, essential mixed cryoglobulinemia, and chronic lymphocytic leukemia.…”

Section: Discussionmentioning

confidence: 99%

Lymphoproliferation in primary sjögren's syndrome. evidence of selective expansion of a b cell subset characterized by the expression of cross‐reactive idiotypes

Shokri

Mageed

Maziak

et al. 1993

Arthritis & Rheumatism

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Objective. To evaluate the possibility that lymphoproliferation in primary Sjogren's syndrome (SS) arises within a subset of B cells.Methods. A panel of monoclonal antibodies (MAb) specific for rheumatoid factor (RF)-associated cross-reactive idiotypes (CRI) and anti-V, and anti-V, subgroup antibodies were used to define the clonality of B lymphocytes undergoing neoplastic transformation in 5 patients with primary SS. Anti-CRI antibodies were also used to study longitudinal variations in serum paraprotein levels and in vitro regulation of IgM and IgM-RF production by peripheral blood lymphocytes. in serum and culture supernatants by enzyme-linked immunosorbent assay. Heavy and light chain isotypes and V, subgroups of the paraproteins were determined by immunoelectrophoresis, immunofixation, and Western blotting.Results. Paraproteins from all patients expressed an epitope associated with VJIIb sub-subgroup of light chains. Three of the paraproteins were cryoglobulins with RF activity, all of which expressed the VJIIbassociated CRI (detected by MAb 17-109) and the V,I-associated CRI (detected by MAb G6 and GS). None of the paraproteins expressed the V,III-associated CRI (detected by MAb B6 and D12). The CRI were consistently expressed over a period of 5-6 years. The anti-CRI and anti-subgroup antibodies substantially inhibited spontaneous production of IgM-RF and IgM by peripheral blood B lymphocytes from 3 of the SS patients.

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Section: Discussionmentioning

confidence: 99%

Lymphoproliferation in primary sjögren's syndrome. evidence of selective expansion of a b cell subset characterized by the expression of cross‐reactive idiotypes

Shokri

Mageed

Maziak

et al. 1993

Arthritis & Rheumatism

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“…9 Large numbers of B-1a cells were found in subjects with Sjö gren's syndrome or rheumatoid arthritis. [10][11][12] B-1a cells were also detected in gingival lesions of subjects with chronic periodontitis. 13 Periodontitis is an infectious disease that affects tooth-supporting structures such as connective tissue attachment and alveolar bone.…”

Section: Introductionmentioning

confidence: 93%

The Sp1 transcription factor binds to the G-allele of the –1087 IL-10 gene polymorphism and enhances transcriptional activation

et al. 2008

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The objectives of this study were to evaluate the influence of the À1087 single nucleotide polymorphism (SNP) on the gene expression of interleukin (IL)-10 and to identify transcription factors binding to this site in B cells. Using electrophoretic mobilityshift assays and nuclear extract from the DG75 B-cell line, we demonstrated that the Sp1 transcription factor bound to the À1087 G-allele of the IL-10 promoter and that the transcription factors PU.1 and Spi-B bound to both the G-and A-alleles. Transient transfections showed that lipopolysaccharide stimulation resulted in a 15-fold increase in promoter activity for the G-allele as compared to a 6-fold increase for the A-allele. Co-transfection with Sp1 expression vector in Sp1-deficient SL2 cells leading to Sp1 binding to the G-allele of the À1087 SNP resulted in increased IL-10 promoter activity. The results suggest a role for Sp1 transcription factor in the activation of IL-10 through the G-allele of the À1087 SNP in response to inflammatory signals.

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“…[3][4][5] Large numbers of B-1a cells were found in subjects with chronic periodontitis, Sjö gren's syndrome and rheumatoid arthritis. [6][7][8][9][10] Interleukin (IL)-10 is an important cytokine in the regulation of immune responses and was earlier considered to be mainly anti-inflammatory. IL-10 is currently, however, recognized as a pro-inflammatory cytokine that promotes a Th2 response as well as B-cell proliferation and antibody production.…”

Section: Introductionmentioning

confidence: 99%

Sp1 binds to the G allele of the−1087 polymorphism in the IL-10 promoter and promotes IL-10 mRNA transcription and protein production

2010

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Interleukin (IL)-10 is an important cytokine in immune regulation and promotes B-cell proliferation and antibody production. High levels of IL-10 were found in subjects with autoimmune diseases. The A to G single nucleotide polymorphism at -1087 of the IL-10 promoter is associated with differences in promoter activity and IL-10 production. The objectives of this study were to analyze differences in the transcription factor binding to the -1087 IL-10 gene polymorphism in B-cells, the influence of the A to G transition on the IL-10 and Sp1 gene expression in B-cells after lipopolysaccharide (LPS) stimulation and the effect of knockdown of Sp1 on IL-10 gene expression. Using B-cell lines obtained from subjects with GG and AA genotypes for the À1087 polymorphism and chromatin immunoprecipitation assay, we showed that the transcription factors PU.1 and Spi-B bound to both G and A alleles, whereas the transcription factor Sp1 only bound to the G allele. LPS stimulation of the B-cells resulted in a larger increase in IL-10 and Sp1 gene expression for GG genotypes than AA genotypes and knockdown of Sp1 gene expression resulted in a decrease in IL-10 mRNA transcription. IL-10 production was higher for the GG genotype than for the AA genotype.

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B cells expressing cd5 are increased in sjögren's syndrome

Cited by 211 publications

References 24 publications

Lymphoproliferation in primary sjögren's syndrome. evidence of selective expansion of a b cell subset characterized by the expression of cross‐reactive idiotypes

Lymphoproliferation in primary sjögren's syndrome. evidence of selective expansion of a b cell subset characterized by the expression of cross‐reactive idiotypes

The Sp1 transcription factor binds to the G-allele of the –1087 IL-10 gene polymorphism and enhances transcriptional activation

Sp1 binds to the G allele of the−1087 polymorphism in the IL-10 promoter and promotes IL-10 mRNA transcription and protein production

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