B Chromosomes in Psalidodon scabripinnis (Characiformes, Characidae) Species Complex

Silva, Duílio Mazzoni Zerbinato de Andrade; Castro, Jonathan Pena; Goes, Caio Augusto Gomes; Utsunomia, Ricardo; Vidal, Mateus Rossetto; Nascimento, Cristiano Neves do; Lasmar, Lucas F; Paim, Fabilene Gomes; Soares, Letícia B; Oliveira, Cláudio; Porto‐Foresti, Fábio; Artoni, Roberto Ferreira; Foresti, Fausto

doi:10.3390/ani12172174

Cited by 6 publications

References 113 publications

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Supernumerary Chromosomes Enhance Karyotypic Diversification of Narrow‐Headed Voles of the Subgenus Stenocranius (Rodentia, Mammalia)

Pavlova,

Romanenko,

Matveevsky

et al. 2024

J Exp Zool Pt B

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The subgenus Stenocranius contains two cryptic species: Lasiopodomys gregalis (subdivided into three allopatrically distributed and genetically well‐isolated lineages A, B, and C) and Lasiopodomys raddei. To identify karyotype characteristics of this poorly studied cryptic species complex, we used comparative cytogenetic analysis of 138 individuals from 41 localities in South Siberia and Mongolia. A detailed description of the L. raddei karyotype and of the L. gregalis lineage С karyotype is presented for the first time. The A chromosome complement of all examined narrow‐headed voles consisted of 2n = 36 and a fundamental number of autosomal arms (FNa) of 50. Between species, patterns of differential staining were similar, though additional C‐heterochromatic blocks were found in L. gregalis lineages; Ag‐positive nucleolar organizers and ribosomal DNA (rDNA) clusters are located on eight and nine acrocentric pairs, respectively. No B chromosomes (Bs) were found in the Early Pleistocene relic L. raddei, while one to five small heterochromatic acrocentric Bs were detected in all L. gregalis lineages; the number and frequency of Bs varied considerably within lineages, but no intraindividual variation was observed. In both species, telomeric repeats were visualized at termini of all chromosomes, including Bs. The number and localization of rDNA clusters on Bs varied among B‐carriers. Immunodetection of several meiotic proteins indicated that meio‐Bs are transcriptionally inactive and have a pattern of meiotic behavior similar to that of sex chromosomes (some homology of Bs to sex chromosomes is supposed). The nature, mechanisms of inheritance and stability of Bs in L. gregalis require further investigation.

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Supernumerary Chromosomes Enhance Karyotypic Diversification of Narrow‐Headed Voles of the Subgenus Stenocranius (Rodentia, Mammalia)

Pavlova,

Romanenko,

Matveevsky

et al. 2024

J Exp Zool Pt B

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A fish frontier? Itatiaia expedition and biodiversity repositories reveal gaps in fish occurrences in Brazil’s high-altitude aquatic ecosystems

Soares Guedes,

Pacheco da Luz,

de Jesus Souza

et al. 2024

Preprint

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Brazil harbors one of the greatest diversities of freshwater fish in the world; however, the presence or absence of fish in high-altitude aquatic ecosystems remains largely unknown. This study aims to investigate fish occurrence on the Itatiaia Plateau (Itatiaia National Park) at altitudes ranging from 2,140 to 2,543 meters, marking one of the highest-altitude fish surveys conducted in Brazil. Additionally, it analyzes gaps in fish distribution above 2,000 meters in Brazil by compiling approximately 1 million occurrence records from digital biodiversity repositories (SpeciesLink, Salve, SIBBr). Results from the Itatiaia expedition and biodiversity repositories converge to indicate a lack of fish records in high-altitude aquatic ecosystems (> 2,000 m) in Brazil. Psalidodon scabripinnis (Jenyns 1842) is the species with the highest recorded altitude (~1,944 m). Challenging climatic conditions, physical barriers to dispersal, isolation, historical absence, sampling gaps, and repository biases may be associated with this lack of fish occurrences. This study highlights gaps in knowledge of fish distribution and the potential for future research to discover previously unknown species or species adapted to high altitudes in Brazil.

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Investigation of Astyanax mexicanus (Characiformes, Characidae) chromosome 1 structure reveals unmapped sequences and suggests conserved evolution

Silva,

Mazzoni Zerbinato Andrade Silva,

Castro

et al. 2024

PLoS ONE

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Natural selection in the cave habitat has resulted in unique phenotypic traits (including pigmentation loss and ocular degeneration) in the Mexican tetra Astyanax mexicanus, considered a model species for evolutionary research. A. mexicanus has a karyotype of 2n = 50 chromosomes, and long-read sequencing and quantitative trait linkage maps (QTLs) have completely reconstructed the reference genome at the chromosomal level. In the current work, we performed whole chromosome isolation by microdissection and total amplification using DOP-PCR and Whole Chromosome Painting (WCP), followed by sequencing on the Illumina NextSeq platform, to investigate the microstructure of the large and conserved metacentric chromosome 1 of A. mexicanus. The sequences aligned to linkage block 3 of the reference genome, as determined by processing the reads with the DOPseq pipeline and characterizing the satellites with the TAREAN program. In addition, part of the sequences was anchored in linkage blocks that have not yet been assigned to the chromosomes. Furthermore, fluorescence in situ hybridization using WCP 1 carried out in other nearby species revealed a high degree of chromosome conservation, which allows us to hypothesize a common origin of this element. The physical mapping of the repetitive marker sequences provided a micro- and macrostructural overview and confirmed their position in chromosome pair 1. These sequences can serve as comparative tools for understanding the evolution and organization of this chromosome in other species of the family in future studies.

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B Chromosomes in Psalidodon scabripinnis (Characiformes, Characidae) Species Complex

Cited by 6 publications

References 113 publications

Supernumerary Chromosomes Enhance Karyotypic Diversification of Narrow‐Headed Voles of the Subgenus Stenocranius (Rodentia, Mammalia)

Supernumerary Chromosomes Enhance Karyotypic Diversification of Narrow‐Headed Voles of the Subgenus Stenocranius (Rodentia, Mammalia)

A fish frontier? Itatiaia expedition and biodiversity repositories reveal gaps in fish occurrences in Brazil’s high-altitude aquatic ecosystems

Investigation of Astyanax mexicanus (Characiformes, Characidae) chromosome 1 structure reveals unmapped sequences and suggests conserved evolution

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