Contribution of CD4+ and CD8+ T lymphocyte subsets to the cytokine secretion patterns induced in mice during sensitization to contact and respiratory chemical allergens

Dearman, Rebecca J.; Moussavi, A; Kemeny, David M.; Kimber, Ian

doi:10.1046/j.1365-2567.1996.d01-778.x

Cited by 111 publications

(61 citation statements)

References 35 publications

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“…There was no evidence for induction of Tc2 cells. However, IFN-g was produced in formaldehyde-sensitized mice by CD4 π Th1 cells and in DNCB-sensitized mice by both CD4 π Th1 cells and CD8 π Tc1 cells (40). These data illustrate that in response to some skin sensitizing chemicals, both CD4 π and CD8 π cell responses are induced, but that this is not necessarily a universal rule.…”

Section: Diversity Of Effector Cell Phenotypes: Experimental Evidencementioning

confidence: 87%

Allergic contact dermatitis: the cellular effectors

2002

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Contact hypersensitivity reactions are mediated by lymphocytic effector cells. Until recently it was believed that the most important of these were CD4 π T lymphocytes. However, there is growing evidence that in many instances the predominant effector cell may be a CD8 π T lymphocyte, with in some instances CD4 π cells performing a counter-regulatory function. Here we review the roles of CD4 π T helper (Th) cells and CD8 π T cytotoxic (Tc) cells, and their main functional subpopulations (respectively, Th1 and Th2 cells and Tc1 and Tc2 cells) in the elicitation of contact hypersensitivity reactions and consider the implications of effector cell selectivity for the biology of allergic contact dermatitis.

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Section: Diversity Of Effector Cell Phenotypes: Experimental Evidencementioning

confidence: 87%

Allergic contact dermatitis: the cellular effectors

2002

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“…The converse type 2 cytokine secretion profile was provoked by the chemical respiratory allergen trimellitic anhydride (TMA) (11,12). These polarized cytokine phenotypes take time to mature, with LNC isolated 3 days after initiation of exposure expressing both type 1 and type 2 cytokines following treatment with either chemical contact or respiratory allergens (13)(14)(15). These data suggest that the molecular signature of chemical allergens may be being misread by the immune system as pathogens, resulting in Th1-or Th2-type polarization.…”

Section: T He Development Of Polarized Immune Responses Is Orchestratmentioning

confidence: 99%

Impact of Cutaneous IL-10 on Resident Epidermal Langerhans’ Cells and the Development of Polarized Immune Responses

Cumberbatch

Clelland

Dearman

et al. 2005

The Journal of Immunology

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Prolonged topical exposure of BALB/c mice to chemical contact and respiratory allergens stimulates, respectively, preferential Th1- and Th2-type responses with respect to serum Ab isotype and cytokine secretion phenotypes displayed by draining lymph node cells. We now report that differential cytokine secretion patterns are induced rapidly in the skin following first exposure to the contact allergen 2,4-dinitrochlorobenzene (DNCB) and the respiratory sensitizer trimellitic anhydride (TMA). TMA induced early expression of IL-10, a cytokine implicated in the negative regulation of Langerhans cell (LC) migration, whereas exposure to DNCB resulted in production of the proinflammatory cytokine IL-1β. Associated with this, TMA provoked LC migration with delayed kinetics compared with DNCB, and local neutralization of IL-10 caused enhanced LC mobilization in response to TMA with concomitant up-regulation of cutaneous IL-1β. We hypothesize that these differential epidermal cytokine profiles contribute to the polarization of immune responses to chemical allergens via effects on the phenotype of activated dendritic cells arriving in the draining lymph node. Thus, TMA-exposed dendritic cells that have been conditioned in vivo with IL-10 (a potent inhibitor of the type 1-polarizing cytokine IL-12) are effective APCs for the development of a Th2-type response.

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“…However, until now, there is no validated method to assess the potentials of chemicals to induce respiratory sensitization. Several groups of researchers have attempted to create a model to test chemicals' potential to induce respiratory sensitization [7][8][9][10][11]. In these attempts, the researchers use different animal models, including, rats, mice, and guinea pigs and assess lung ventilatory as well as immunological determinants that could predict respiratory sensitization potentials.…”

Section: Introductionmentioning

confidence: 99%

Comparative Studies of Lymph Node Cell Subpopulations and Cytokine Expression in Murine Model for Testing the Potentials of Chemicals to Induce Respiratory Sensitization

Tarkowski

Kur²,

Polakowska³

et al. 2008

International Journal of Occupational Medicine and Environmental Health

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Objectives: To investigate immunological changes in lymph nodes based on expression of cell-specific receptors and cytokine expression profile and accompanying inflammatory reactions in lungs of mice treated with chemicals of known potentials to induce respiratory sensitization and those in which activity in this regard is unclear. Materials and Methods: On day 1 and 7, Balb/c mice received toluene-2,4-diisocyanate (TDI), trimellitic anhydride (TMA), 1-chloro-2,4-dinitrobenzene (DNCB), glutaraldehyde (GA), formaldehyde (FA), benzalkonium chloride (ChB) or vehicle. On day 14, they received a single intranasal instillation with the same chemical or vehicle. On day 15, auricular lymph nodes (LN) were excised and used for analyzes of T-, B-cells, expression of CD44 and for the estimation of IL-4 and IFN-γ production after in vitro stimulation with concanavalin A (ConA) and also for IL-4 and IFN-γ mRNA expression analyses using Real-Time PCR. Inflammatory changes in lungs were observed by estimation of TNF-α and MIP-2 concentrations and cell numbers and their type in BAL. Results: There were no significant changes in cell subpopulations of T helper cells in LN. The percent of B cells was significantly increased after treatment with DNCB, TDI, and GA. Increased expression of CD44 on T cells was also observed. Both IL-4 and IFN-γ were found increased in TDI-and FA-treated mice, while only IL-4 was increased in TMA-treated mice. Real-Time PCR analyses, however, showed increased IL-4 mRNA expression for TDI-and TMA-, and IFN-γ mRNA expression for DNCB-treated mice. We haven't observed significant changes in inflammatory reactions in the lungs of exposed animals. Conclusions: Studying immunological changes with first determining the activation status of T cells followed by analyzes of expression of mRNA for Th1 and Th2 cytokines in murine model could be a useful method for assessment of the potentials of chemicals to induce respiratory sensitization but is not sufficient. Addition of ventilatory measurements, but not necessarily inflammatory reactions, could complete the model.

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Contribution of CD4⁺ and CD8⁺ T lymphocyte subsets to the cytokine secretion patterns induced in mice during sensitization to contact and respiratory chemical allergens

Cited by 111 publications

References 35 publications

Allergic contact dermatitis: the cellular effectors

Allergic contact dermatitis: the cellular effectors

Impact of Cutaneous IL-10 on Resident Epidermal Langerhans’ Cells and the Development of Polarized Immune Responses

Comparative Studies of Lymph Node Cell Subpopulations and Cytokine Expression in Murine Model for Testing the Potentials of Chemicals to Induce Respiratory Sensitization

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