<b>Cytomorphometric analysis of squames obtained from normal oral mucosa and lesions of oral leukoplakia and squamous cell carcinoma</b>

Ramaesh, Thaya; Mendis, B. R. R. N.; Ratnatunga, N; Thattil, R. O.

doi:10.1111/j.1600-0714.1998.tb02099.x

Cited by 71 publications

(81 citation statements)

References 15 publications

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“…Measurements of NA and CA have been used as important parameters for detecting alterations in the epithelial tissues [9][10][11][12][13][14] . In our study, two situations were present: i) a reduction in both NA and CA (region 1), and ii) a reduction only in the NA (region 2).…”

Section: Resultsmentioning

confidence: 99%

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Preclinical Alterations of Oral Epithelial Cells in Contact With Orthodontic Appliances

Arruda¹,

Trevilatto²,

Camargo³

et al. 2011

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Aim. This study evaluated the behavior of oral epithelial cells in contact with orthodontic appliances. Methods. Oral epithelial cells of clinically normal buccal mucosa were obtained by liquid-based exfoliative cytology from 22 orthodontic patients. The following regions were evaluated: a) oral mucosa exposed to friction from orthodontic brackets; b) oral mucosa exposed to friction from the tube on the orthodontic band, and c) oral mucosa not exposed to friction (control area). Nuclear (NA) and cytoplasmic (CA) areas and NA/CA ratio were assessed by an image analysis system. Cell morphology and cellularity were also analyzed by Papanicolaou technique.Results. The NA of the cells in contact with orthodontic brackets and bands were smaller than the control area. Cells in contact with the brackets showed the greatest reduction in CA in comparison with the cells subjected to friction from the tube, and the control group. Smears classified as type I predominated in all regions analyzed, although type II were predominant, together with superficial epithelial cells, mainly in the oral mucosa in contact with the band tube.Conclusion. Preclinical alterations in the epithelial cells of oral mucosa, caused by orthodontic appliances, are reduction in NA, increase in cell keratinization and inflammatory features, especially in the band tube area.

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Section: Resultsmentioning

confidence: 99%

“…Several studies have reported the effect of different types of diseases and aggressive agents on oral mucosa, such as alcohol, and tobacco 10,12,[20][21][22][23][24][25] . However, studies reporting physical trauma associated to use of orthodontic appliances are still scarce 8 .…”

Section: Resultsmentioning

confidence: 99%

Preclinical Alterations of Oral Epithelial Cells in Contact With Orthodontic Appliances

Arruda¹,

Trevilatto²,

Camargo³

et al. 2011

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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“…Ramaesh et al, (1998) [2] evaluated that the mean CD and ND values in normal smears varies from 43.29 µ-59.18 µm and 7.00-9.2 µm respectively using calibrated eye piece for measurement. The values of CD in our study were slightly more.…”

Section: Discussionmentioning

confidence: 99%

Quantitative exfoliative cytology of squames obtained from iron defi ciency anemia and healthy individuals

Vanishree¹,

Kale²,

Hallikerimath³

et al. 2014

JCRI

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Background: Iron deficiency anemia is the most common nutritional deficiency of world affecting people of all ages and economic groups. Previous studies on the effects of iron deficiency anemia by exfoliative oral cytology have produced conflicting results but application of quantitative parameters such as nuclear diameter (ND) and cytoplasmic diameter (CD) and nuclear to cytoplasmic ratio (N/C) has shown to be significant in the diagnosis of oral lesions. The present study was undertaken to assess the morphometric changes in CD and ND of squames obtained from buccal mucosa of patients with iron deficiency anemia and also to assess the effect of serum ferritin levels on cellular morphometry. Materials and Methods: Thirty cases of iron deficiency anemia and 30 cases of control group were selected for the study. The control group consisted of people without clinical symptoms of anemia, normal hematological and serum ferritin levels. Scrapings were taken from the buccal mucosa of the iron deficiency anemia patients and control groups and stained with Papanicolaou stain. ND and CD were measured using image analyzer. Results: CD and ND values of the control group were found to be in the range of 65.32-75.39 µ and 8.10-9.40 µ respectively. CD values of iron deficiency were 55.05-64.12 µ with the mean CD value of 59.77 µ and ND values were 8.69-11.24 µ with mean ND values of 9.88 µ. On correlating the serum ferritin and red cell parameters with the CD and ND values of iron deficiency anemia showed positive correlation. Conclusions: The decrease in cytoplasmic diameter and increase in ND in iron deficiency anemia and progressive decrease in CD with decrease in serum ferritin levels suggested that iron deficiency causes significant changes in oral exfoliative cells. Cytomorphometric analysis of smears is useful in detecting the changes in iron deficiency anemia.

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“…Uma área nuclear reduzida e uma área citoplasmática aumentada são fortes indicadores precoces da transformação maligna 18 . A partir deste conhecimento, um grande número de estudos foi realizado usando esta técnica descrita para avaliar a influência de diversos fatores sistêmicos e externos sobre as áreas nuclear e citoplasmática e a relação núcleo/citoplasma 22 . Vários fatores de risco vêm sendo apontados com relação ao desenvolvimento do câncer bucal.…”

Section: áRea Média Do Citoplasma (Micra)unclassified

“…Entretanto, já é sabido que quantidades excessivas de espécies de nitrogênio reativo produzidas durante a inflamação crônica podem desempenhar um papel importante na carcinogênese devido a danos ao DNA 27 . Na Índia, o vício de mascar betel associado ou não com tabaco é capaz de gerar uma redução significativa na área do citoplasma e um aumento na área do núcleo de células da mucosa bucal 8,22 . O uso do betel é comum em países orientais e tem se mostrado capaz de provocar o câncer bucal.…”

Section: áRea Média Do Citoplasma (Micra)unclassified

Análise Citomorfométrica de Esfregaços Bucais de Fumantes Obtidos pela Citologia Esfoliativa em Base Líquida

Ferreira¹,

Batista²,

Ignácio³

et al. 2008

Pesq Bras Odontoped Clin Integr

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RESUMO DESCRIPTORS DESCRITORESTabagismo; Neoplasia bucal; Citologia; Mucosa bucal.Smoking; Mouth neoplasia; Cytology; Mouth mucosa. Cytomorphometric Analysis of Oral Smears from Smokers Obtained by Liquid Base Exfoliative CytologyObjective: To evaluate the effect of manufactured cigarettes on the nuclear area (NA), cytoplasmic area (CA), and nuclear/ cytoplasmic ratio (NA/CA) in oral mucosa epithelial cells in young adults. Method: Oral smears were collected by liquid-based exfoliative cytology from clinically healthy jugal mucosa in 58 individuals (28 smokers and 30 non-smokers). Glass slides were submitted to laboratory processing, stained by the Papanicolaou technique and analyzed using an image-analysis system, according to the methodology proposed by Ogden et al. (1990). Results:The mean NA values for the experimental and control groups were 1530.6ì m 2 and 1498.6ì m 2 , respectively, while the mean CA values for the experimental and control groups were, respectively, 63780.3ì m 2 and 62929.9ì m 2 . The NA/CA ratio for the experimental group was 0.023 versus 0.024 for the control group. The Student's t-test showed no statistically significant difference for the studied variables (p>0.05). Conclusion:The consumption of manufactured cigarettes was not capable of inducing significant morphometric alterations in the oral epithelial cells of the individuals evaluated. This fact reinforces the hypothesis that long-term exposure to tobacco is needed before cellular alterations occur.Objetivo: Avaliar o efeito do uso de cigarro industrializado sobre as áreas nuclear (AN), citoplasmática (AC) e na relação núcleo/ citoplasmática (AN/AC) de células epiteliais da mucosa bucal de adultos jovens. Método: Esfregaços da mucosa jugal saudável foram obtidos pela técnica da citologia esfoliativa em base líquida de 58 indivíduos (28 indivíduos fumantes e 30 não fumantes). As lâminas foram processadas em laboratório, coradas pela técnica do Papanicolaou e analisadas utilizando um sistema analisador de imagens seguindo a metodologia preconizada por Ogden et al. (1990). Resultados: A média da AN para os grupos experimental e controle foram, respectivamente, 1530,6µm 2 e 1498,6µm 2 . A variável AC apresentou as seguintes médias: 63780,3µm 2 (experimental) e 62929,9µm 2 (controle). A relação AN/AC para o grupo experimental foi de 0,023, enquanto que para o grupo controle foi de 0,024. O teste t de student demonstrou não haver diferença estatisticamente significativa para as variáveis estudadas (p>0,05). Conclusão: O consumo de cigarro industrializado não foi capaz de induzir alterações morfométricas significativas nas células da mucosa bucal dos indivíduos estudados. Este fato reforça a hipótese de que há a necessidade de um tempo de exposição prolongada para que as modificações celulares ocorram.

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Cytomorphometric analysis of squames obtained from normal oral mucosa and lesions of oral leukoplakia and squamous cell carcinoma

Cited by 71 publications

References 15 publications

Preclinical Alterations of Oral Epithelial Cells in Contact With Orthodontic Appliances

Preclinical Alterations of Oral Epithelial Cells in Contact With Orthodontic Appliances

Quantitative exfoliative cytology of squames obtained from iron defi ciency anemia and healthy individuals

Análise Citomorfométrica de Esfregaços Bucais de Fumantes Obtidos pela Citologia Esfoliativa em Base Líquida

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