<b>Hydration of Deoxyribonucleic Acid. II. An Infrared Study</b>

Falk, Michael; Hartman, K.A.; Lord, R. C.

doi:10.1021/ja00887a004

Cited by 326 publications

(184 citation statements)

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“…It is known that the addition of water to dry DNA results first in the binding of H 2 O to the phosphate group. In other words, the ionic phosphate group is responsible for the majority of the first layer of hydration (Falk et al, 1963). Ptasińska and Sanche (2007) showed that the formation of a DNA-H 2 O complex permits the formation of a transient anion located on the phosphate group via LEE impact, and consequently, new dissociative transient anion states, which can cause an enhancement in the DNA damage.…”

Section: Discussionmentioning

confidence: 99%

Induction of strand breaks in DNA films by low energy electrons and soft X-ray under nitrous oxide atmosphere

Alizadeh

Sanche

2012

Radiation Physics and Chemistry

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Five-monolayer (5ML) plasmid DNA films deposited on glass and tantalum substrates were exposed to Al K α X-rays of 1.5 keV under gaseous nitrous oxide (N 2 O) at atmospheric pressure and temperature. Whereas the damage yields for DNA deposited on glass are due to soft X-rays, those arising from DNA on tantalum are due to both the interaction of low energy photoelectrons from the metal and X-rays. Then, the differences in the yields of damage on glass and tantalum substrates, essentially arises from interaction of essentially low-energy electrons (LEEs) with DNA molecules and the surrounding atmosphere. The G-values (i.e., the number of moles of product per Joule of energy absorbed) for DNA strand breaks induced by LEEs (G LEE ) and the lower limit of G-values for soft X-ray photons (G XL ) were calculated and the results compared to those from previous studies under atmospheric conditions and other ambient gases, such as N 2 and O 2 . Under N 2 O, the G-values for loss of supercoiled DNA are 103±15 nmol/J for X-rays, and 737±110 nmol/J for LEEs. Compared to corresponding values in an O 2 atmosphere, the effectiveness of X-rays to damage DNA in N 2 O is less, but the G value for LEEs in N 2 O is more than twice the corresponding value for an oxygenated environment. This result indicates a higher effectiveness for LEEs relative to N 2 and O 2 environments in causing SSB and DSB in an N 2 O environment. Thus, the previously observed radiosensitization of cells by N 2 O may not be only due to OH • radicals but also to the reaction of LEE with N 2 O molecules near DNA. The previous experiments with N 2 and O 2 and the present one demonstrate the possibility to investigate damage induced by LEEs to biomolecules under various type of surrounding atmospheres.

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Section: Discussionmentioning

confidence: 99%

Induction of strand breaks in DNA films by low energy electrons and soft X-ray under nitrous oxide atmosphere

Alizadeh

Sanche

2012

Radiation Physics and Chemistry

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“…The collection stress does not seem t o support this theory. An alternative explanation is possible in terms of the formation of hydrates of key biological molecules (Falk, Hartman & Lord, 1962, 1963a, b ; Falk, 1964) with some hydrates being formed in a semi-reversible manner, while another is that enzyme activation may occur at certain RH values. This type of behaviour has been demonstrated (Couper, Eley & Hayward, 1955).…”

Section: The Action Of Protecting Agentsmentioning

confidence: 99%

The Survival of Escherichia coli sprayed into Air and into Nitrogen from Distilled Water and from Solutions of Protecting Agents, as a Function of Relative Humidity

Cox

1966

Journal of General Microbiology

103

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SUMMARYThe survival in air as a function of relative humidity (RH) and aerosol age is reported for Escherichia coli (strains B and Jepp) and for Serratia marcescens (strain U K 8 ) sprayed from suspensions in distilled water and in solutions of dextran and of raffinose. The survival in nitrogen ( > 99.9 yo) of E . coli (strains B, Jepp and commune) is given as a function of RH, when sprayed from suspension in distilled water and in raffinose solution. The results show that E . coli in the aerosol was subject to at least three stresses, an air stress, an RH stress, and a collection stress. The air stress accounted for loss of viability at low RH and the RH stress occured a t high RH and is expressed as RH ranges in which E . coli rapidly lost viability. The survival, especially a t high RH, depended upon the composition of the collecting fluid. At high RH a range of RH was found in which the bacteria were unstable when collected in phosphate buffer. Addition of raffinose (chosen as a typical protecting agent) to the spray fluid and the addition of M-sucrose to the collecting fluid eliminated the instability for E . coli (commune) and decreased it for the other two strains.

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“…Swelling of the DNA duplex occurs as the double helix becomes completely hydrated with approximately 15-20 water molecules per nucleobase [51]. This swelling would allow the neutral piperidine easier accessibility to the nucleobases and promote the disruption of the hydrogen bonding as described by the proposed mechanism.…”

Section: Methodsmentioning

confidence: 96%

Solution composition and thermal denaturation for the production of single-stranded PCR amplicons: Piperidine-induced destabilization of the DNA duplex?

Mangrum

Flora

Muddiman

2002

J. Am. Soc. Mass Spectrom.

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Strategies to produce single-stranded PCR amplicons for detection by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR-MS) were investigated using modified electrospray solutions and by thermally denaturing the duplex structures with a resistively heated electrospray ionization source. A synthetic 20-mer oligonucleotide annealed to its complementary strand was used as a model system for initial experiments. Electrospray solutions were altered by varying the relative proportion of aqueous phase in efforts to induce destabilization of the double helix. When the electrospray solution contains a 25% aqueous content, the 20-mer oligonucleotide is detected in its double-stranded form. Increasing the proportion of aqueous phase in the electrospray solution to 60% destabilized the double helix, resulting in the detection of only single-stranded species. This strategy was extended to an 82-bp polymerase chain reaction (PCR) product derived from the human tyrosine hydroxylase gene (HUMTH01). In efforts to destabilize the 82-bp PCR product, electrospray solutions reaching 70% aqueous content were necessary to promote the detection of only single-stranded amplicons. Implementation of the resistively heated transfer line and an electrospray solution in which the oligonucleotide is on the threshold of duplex stability allowed for double-stranded and single-stranded species to be generated from the same ESI solutions at both ambient and elevated transfer line temperatures, respectively, without disruption of the electrospray process. The volatile base piperidine, present at 20 mM concentrations in the electrospray solution, was found to play a critical role in the formation of single-stranded species at the higher aqueous percentages and a duplex destabilization mechanism has been proposed. (J Am Soc Mass Spectrom 2002, 13, 232-240)

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Hydration of Deoxyribonucleic Acid. II. An Infrared Study

Cited by 326 publications

References 0 publications

Induction of strand breaks in DNA films by low energy electrons and soft X-ray under nitrous oxide atmosphere

Induction of strand breaks in DNA films by low energy electrons and soft X-ray under nitrous oxide atmosphere

The Survival of Escherichia coli sprayed into Air and into Nitrogen from Distilled Water and from Solutions of Protecting Agents, as a Function of Relative Humidity

Solution composition and thermal denaturation for the production of single-stranded PCR amplicons: Piperidine-induced destabilization of the DNA duplex?

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