<b><i>In vitro</i></b>genotoxicity of fly ash leachate in earthworm coelomocytes

Manerikar, R. S.; Apte, A. A.; Ghole, Vikram S.

doi:10.1080/02772240701458451

Cited by 16 publications

(2 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Regarding the first point, the contaminants content of ashes could be incriminated. Indeed, the toxic and mutagenic effects of high concentrations of FAs on different tests organisms (e.g., Ali et al, 2004;Chakraborty and Mukherjee, 2009;Manerikar et al, 2008;Papadimitriou et al, 2008;Van Maanen et al, 1999;Voelkel et al, 2003) would be linked, according to authors, to organic contaminants or TEs present in such FAs. In our study, both ashes contained TEs (Table 1).…”

Section: Effects Of the Addition Of Fly Ashes To Metal-contaminated Smentioning

confidence: 97%

Effects of field metal-contaminated soils submitted to phytostabilisation and fly ash-aided phytostabilisation on the avoidance behaviour of the earthworm Eisenia fetida

Demuynck

Succiu

Grumiaux

et al. 2014

Ecotoxicology and Environmental Safety

View full text Add to dashboard Cite

Section: Effects Of the Addition Of Fly Ashes To Metal-contaminated Smentioning

confidence: 97%

Effects of field metal-contaminated soils submitted to phytostabilisation and fly ash-aided phytostabilisation on the avoidance behaviour of the earthworm Eisenia fetida

Demuynck

Succiu

Grumiaux

et al. 2014

Ecotoxicology and Environmental Safety

View full text Add to dashboard Cite

“…There are a number of reports on soil pollution (Love et al, 2013) or ground-water contamination (Rai and Szelmeczka, 1990) caused by coal fly ash. To examine the hazardous nature of such fly ash, a freshwater fish (Ali et al, 2004(Ali et al, , 2007 Daphnia magna (Tsiridis et al, 2012), strains of the bacterium Salmonella Typhimurium (Ames test), and earthworms (Manerikar et al, 2008) are used as bioassay test organisms. According to Spears and Lee (2004), approximately 70% of coal fly ash produces a neutral or alkaline leachate.…”

Section: Introduction Introductionmentioning

confidence: 99%

Effect of Coal Fly Ash Leachate on the Bioluminescence Intensity of Vibrio fischeri

Ikeda¹,

Kostova²,

Sekine³

et al. 2016

CCGP

View full text Add to dashboard Cite

A B S T R A C TCoal fly ash is a residue of coal-fired thermoelectric power plants (TPPs) and is mostly dumped in ash ponds or landfill sites, even though it potentially contains significant amounts of water-soluble hazardous contaminants. Bioassay using the bioluminescent bacterium Vibrio fischeri is known to be applicable for assessing the short-term and sublethal toxicity of complex mixtures without the need for precise chemical characterization. However, this type of bioassay is potentially adversely influenced by the pH-induced protein denaturation of cells. Because coal fly ash leachates often have alkaline or acidic properties, when applying the V. fischeri-based bioassay to the samples, we need to know potential effect of the leachates on the bioluminescence of the bacteria. This study accordingly aimed to investigate the feasibility of applying the V. fischeri bioassay to coal fly ash leachate as a screening method. Fly ash samples were collected from 12 TPPs located in three East European countries: Bulgaria, Greece, and Serbia. The fly ash samples were prepared in sterilized distilled water by ultrasonic extraction and filtration using 0.45-mmΦ membrane filters. The filtrates were then mixed with a solution of the test bacterium. The bioluminescence intensity was measured using a luminometer. The results showed the ostensible influence of pH on bioluminescence intensity pronounced when following the typical protocol using a 5.0-g/L solid:liquid ratio. Accordingly, the pH of water extracts should be adjusted to within a range of 6 to 9 by dilution to observe the inhibition of bioluminescence by coal fly ash leachate as the objective endpoint.

show abstract

Adverse effects of fly ashes used as immobilizing agents for highly metal-contaminated soils on Xenopus laevis oocytes survival and maturation—a study performed in the north of France with field soil extracts

Marchand¹,

Demuynck²,

Slaby³

et al. 2019

Environ Sci Pollut Res

View full text Add to dashboard Cite

In vitrogenotoxicity of fly ash leachate in earthworm coelomocytes

Cited by 16 publications

References 40 publications

Effects of field metal-contaminated soils submitted to phytostabilisation and fly ash-aided phytostabilisation on the avoidance behaviour of the earthworm Eisenia fetida

Effects of field metal-contaminated soils submitted to phytostabilisation and fly ash-aided phytostabilisation on the avoidance behaviour of the earthworm Eisenia fetida

Effect of Coal Fly Ash Leachate on the Bioluminescence Intensity of Vibrio fischeri

Adverse effects of fly ashes used as immobilizing agents for highly metal-contaminated soils on Xenopus laevis oocytes survival and maturation—a study performed in the north of France with field soil extracts

Contact Info

Product

Resources

About