B- myb is an essential regulator of hematopoietic stem cell and myeloid progenitor cell development

Abstract

The B-myb (MYBL2) gene is a member of the MYB family of transcription factors and is involved in cell cycle regulation, DNA replication, and maintenance of genomic integrity. However, its function during adult development and hematopoiesis is unknown. We show here that conditional inactivation of B-myb in vivo results in depletion of the hematopoietic stem cell (HSC) pool, leading to profound reductions in mature lymphoid, erythroid, and myeloid cells. This defect is autonomous to the bone marrow and is first evident in stem cells, which accumulate in the S and G 2 /M phases. B-myb inactivation also causes defects in the myeloid progenitor compartment, consisting of depletion of common myeloid progenitors but relative sparing of granulocyte-macrophage progenitors. Microarray studies indicate that B-myb-null LSK + cells differentially express genes that direct myeloid lineage development and commitment, suggesting that B-myb is a key player in controlling cell fate. Collectively, these studies demonstrate that B-myb is essential for HSC and progenitor maintenance and survival during hematopoiesis. myeloipiesis | myelodisplastic syndrome

Inactivation of 3- hydroxybutyrate dehydrogenase 2 delays zebrafish erythroid maturation by conferring premature mitophagy

“…6 A and B). These results are consistent with the notion that B-myb is important for erythropoiesis (27). The "leading edge" genes, the subset of the B-myb gene list that account for the enrichment, included transcription factors (Fig.…”

“…Mitochondrial clearance is unique to erythrocytes in hematopoietic cell differentiation. Removal of mitochondria also marks the transition of reticulocytes to mature erythrocytes (27). Retention of mitochondria leads to excess cell death.…”

“…MYB family comprises A-myb, B-myb, C-myb, and V-myb transcription factors (30). Among all these members, only B-myb and C-myb are implicated in hematopoiesis (27,31,32). Global genetic ablation of B-myb results in embryonic lethality; however, conditional deletion results in loss of the hematopoietic stem cell pool and consequent pancytopenia (28).…”

Low Molecular Weight Fraction of Commercial Human Serum Albumin Induces Morphologic and Transcriptional Changes of Bone Marrow-Derived Mesenchymal Stem Cells

“…A doubling in signal was deemed significant for this assay, and a representative experiment is presented in Table 2. A number of transcription factors implicated in stem cell differentiation and self-renewal were actively binding oligonucleotide response element probes following LMWF5A treatment, including AP2, COUP-TF, GATA, Gfi-1, Myb, NFAT, PPAR, RXR, SOX2, VDR, and XBP [14][15][16][17][18][19][20][21][22][23][24][25].…”

Use of Gene Expression Analysis for Discrimination of Primary and Secondary Adenocarcinoma of the Liver

“…This protein plays an essential role in the regulation of hematopoiesis and was first described in malignant hematologic disorders such as leukemias and lymphomas, where an aberrant expression or rearrangement is quite common [37].…”