1996
DOI: 10.1046/j.1365-2559.1996.d01-534.x
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Immunoglobulin light chain staining in paraffin‐embedded tissue using a heat mediated epitope retrieval method

Abstract: We have compared light chain immunohistochemistry in reactive lymphoid tissue and a series of paraffin-embedded B-cell lymphomas using standard trypsin digestion with a heat mediated epitope retrieval method. Fifty-seven B-cell lymphomas (18 high grade, 29 low grade and 10 cases of nodular lymphocyte predominance Hodgkin's disease), two reactive lymph nodes and eight tonsils fixed for known times between 12 h and 2 years were studied. Paraffin-embedded tissue was stained with polyclonal anti-kappa and anti-lam… Show more

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Cited by 31 publications
(13 citation statements)
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“…To stain for macrophages, sections were rehydrated through graded alcohols and were subjected to antigen retrieval using trypsin solution (38). Sections were blocked in 10% serum and incubated in a primary rat anti-macrophage antibody (Abcam) overnight.…”
Section: Methodsmentioning
confidence: 99%
“…To stain for macrophages, sections were rehydrated through graded alcohols and were subjected to antigen retrieval using trypsin solution (38). Sections were blocked in 10% serum and incubated in a primary rat anti-macrophage antibody (Abcam) overnight.…”
Section: Methodsmentioning
confidence: 99%
“…For many years, immunohistochemical detection of light chain restriction has served as diagnostic marker for clonal B-cell proliferations. This technique, however, is not applicable in B-cell lymphoma that lack a plasmacellular infi ltrate [ 44,45 ] .…”
Section: Clonality Analysis In Cutaneous B-cell Lymphomasmentioning
confidence: 99%
“…Its use in other types of B-cell lymphomas requires a very sensitive technique, and is not reproducibly performed in many laboratories. 16 However, Southern blot analysis of Ig-and TCR-gene rearrangements has been considered the gold standard method for clonality testing. 10,[17][18][19] Despite its high reliability, the main drawback of Southern blot analysis is the necessity for large amounts of high molecular weight DNA extracted from fresh or frozen tissue, that is often not available in daily diagnostic practice.…”
Section: Introduction: Relevance Of Clonality Testingmentioning
confidence: 99%