Local immune response in Helicobacter pylori‐infected cats and identification of H. pylori in saliva, gastric fluid and faeces

Fox, James G.; Perkins, Sid; Yan, Lili; Shen, Zeli; Attardo, L; Pappo, Jacques

doi:10.1046/j.1365-2567.1996.d01-677.x

Cited by 63 publications

(35 citation statements)

References 58 publications

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“…S6 -S8, Supporting Information). The employed anti-IgA antibody (clone code: IgA5-3B) has high-specificity for the human IgA (which is reported by previous reports), 28,29 indicating that the response to human IgA is based solely on the immune-interaction between the anti-IgA antibody and the human IgA on the extended-gate electrode. The addition of the human IgA over 10 μg/mL induced a saturated response because the anti-IgA antibody is fully bound to the human IgA.…”

Section: Resultssupporting

confidence: 59%

An Organic Field-effect Transistor with an Extended-gate Electrode Capable of Detecting Human Immunoglobulin A

et al. 2015

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We herein report on the development of an extended-gate type organic field-effect transistor (OFET)-based immunosensor for the detection of human immunoglobulin A (IgA). The titration results of IgA exhibited shifts in the transfer characteristics of the OFET sensor device with increasing IgA concentration. A linear detection range from 0 to 10 μg/mL was realized with a detection limit of 2.1 μg/mL, indicating that the OFET-based immunosensor can be potentially applied to the monitoring of infectious diseases and psychological stress in daily life.

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Section: Resultssupporting

confidence: 59%

An Organic Field-effect Transistor with an Extended-gate Electrode Capable of Detecting Human Immunoglobulin A

et al. 2015

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“…Helicobacter pylori infection in cats is associated with lymphofollicular gastritis, with sub-mucosal lymphoid follicles distributed most frequently in the antrum of the stomach. The gastric lymphoid follicles consist mainly of IgM + B cells surrounded by clusters of CD4 + and CD8 + T cells [21]. More recent studies [47] have sought to characterise the cellular and cytokine changes in gastric tissues that occur during the early stage of infection in cats.…”

Section: Mucosal Cytokines and The Response To Mucosal Infectionmentioning

confidence: 99%

Mucosal defence along the gastrointestinal tract of cats and dogs

Stokes

Waly

2006

Vet. Res.

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-Diseases that are associated with infections or allergic reactions in the gastrointestinal and respiratory tracts are major causes of morbidity in both cats and dogs. Future strategies for the control of these conditions require a greater understanding of the cellular and molecular mechanisms involved in the induction and regulation of responses at the mucosal surfaces. Historically, the majority of the fundamental studies have been carried out in rodents or with tissues obtained from man, but the expanding range of reagents available for the study of farm and companion animals provides opportunities for study in a wider range of animals including cats and dogs. To date, these studies have tended to be focussed on characterising the cellular distributions in healthy animals and in groups of cats and dogs identified as having an increased risk of mucosal disturbance. Where species comparisons of mucosal immune systems have been made, the results have tended to be divided between monogastric and ruminant animals. It is then not surprising that the mucosal immune systems of both cats and dogs bear greatest similarity to that documented for man and pigs.

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“…Gastric secretions were collected with absorbent wicks positioned longitudinally in the gastric lumen (49), after extensive rinsing with PBS containing 0.2 mM 4-(2-aminoethyl)-benzenesulfonyl fluoride (Calbiochem, La Jolla, CA), 1 g of aprotinin per ml, 10 mM leupeptin (Sigma-Aldrich), and 3.25 M Bestatin (Boehringer Mannheim, Indianapolis, IN) protease inhibitor. For extraction of gastric secretions from the wick, 0.5 ml of protease inhibitor containing 5% nonfat dry milk was added to each sample tube containing two wicks, vortexed extensively, and then frozen at Ϫ70°C until it was analyzed.…”

Section: Serum and Gastric Samplesmentioning

confidence: 99%

Helicobacter pylori-Specific Antibodies Impair the Development of Gastritis, Facilitate Bacterial Colonization, and Counteract Resistance against Infection

Akhiani

Schön

Franzén

et al. 2004

The Journal of Immunology

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In recent years, Abs have been considered a correlate rather than an effector of resistance against Helicobacter pylori infection. However, it is still poorly understood to what extent Ab production correlates with gastric immunopathology. Here we report that Abs not only are dispensable for protection, but they are detrimental to elimination of the bacteria and appear to impair gastric inflammatory responses. We found that the initial colonization with H. pylori bacteria was normal in the B cell-deficient (μMT) mice, whereas at later times (>8 wk) most of the bacteria were cleared, concomitant with the development of severe gastritis. In contrast, wild-type (WT) mice exhibited extensive bacterial colonization and only mild gastric inflammation, even at 16 wk after inoculation. Oral immunizations with H. pylori lysate and cholera toxin adjuvant stimulated comparable levels of protection in μMT and WT mice. The level of protection in both strains correlated well with the severity of the postimmunization gastritis. Thus, T cells were responsible for the gastritis, whereas Abs, including potentially host cell cross-reactive Abs, were not involved in causing the gastritis. The T cells in μMT and WT mice produced high and comparable levels of IFN-γ to recall Ag at 2 and after 8 wk, whereas IL-4 was detected after 8 wk only, indicating that Th1 activity dominated the early phase of protection, whereas later a mixed Th1 and Th2 activity was seen.

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**Local immune response in Helicobacter pylori‐infected cats and identification of H. pylori in saliva, gastric fluid and faeces**

Cited by 63 publications

References 58 publications

An Organic Field-effect Transistor with an Extended-gate Electrode Capable of Detecting Human Immunoglobulin A

An Organic Field-effect Transistor with an Extended-gate Electrode Capable of Detecting Human Immunoglobulin A

Mucosal defence along the gastrointestinal tract of cats and dogs

Helicobacter pylori-Specific Antibodies Impair the Development of Gastritis, Facilitate Bacterial Colonization, and Counteract Resistance against Infection

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