B-lymphopoiesis gains sensitivity to subsequent inhibition by estrogens during final phase of fetal development

Hlobeňová, Tereza; Šefc, Luděk; Chang, Ko‐Tung; Savvulidi, Filipp; Michalova, Jana; Nečas, E

doi:10.1016/j.dci.2011.07.009

Cited by 6 publications

(3 citation statements)

References 29 publications

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“…This finding likely reflects the suppressive effect of oestrogens on lymphopoiesis derived from transplanted wild-type bone marrow in female mice. The suppressive action of oestrogens was reported by Pelichovská et al (2008) and Hlobeňová et al (2012). These results confirmed the previous demonstration that transplanted CD45.1 cells contributed more to lymphoid cells than to the granulocytes and monocytes, as report-ed in Faltusová et al (2018).…”

Section: Discussionsupporting

confidence: 90%

T-lymphopoiesis is Severely Compromised in Ubiquitin-Green Fluorescent Protein Transgenic Mice

Faltusová,

Báječný,

Heizer

et al. 2020

Fol. Biol.

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Tagging cells of experimental organisms with genetic markers is commonly used in biomedical research. Insertion of artificial gene constructs can be highly beneficial for research as long as this tagging is functionally neutral and does not alter the tissue function. The transgenic UBC-GFP mouse has been recently found to be questionable in this respect, due to a latent stem cell defect compromising its lymphopoiesis and significantly influencing the results of competitive transplantation assays. In this study, we show that the stem cell defect present in UBC-GFP mice negatively affects T-lymphopoiesis significantly more than B-lymphopoiesis. The production of granulocytes is not negatively affected. The defect in T-lymphopoiesis causes a low total number of white blood cells in the peripheral blood of UBC-GFP mice which, together with the lower lymphoid/myeloid ratio in nucleated blood cells, is the only abnormal phenotype in untreated UBCGFP mice to have been found to date. The defective lymphopoiesis in UBC-GFP mice can be repaired by transplantation of congenic wild-type bone marrow cells, which then compensate for the insufficient production of T cells. Interestingly, the wild-type branch of haematopoiesis in chimaeric UBC-GFP/wild-type mice was more active in lymphopoiesis, and particularly towards production of T cells, compared to the lymphopoiesis in normal wild-type donors.

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Section: Discussionsupporting

confidence: 90%

T-lymphopoiesis is Severely Compromised in Ubiquitin-Green Fluorescent Protein Transgenic Mice

Faltusová,

Báječný,

Heizer

et al. 2020

Fol. Biol.

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“…The number and activity of B lymphocyte precursors in the bone marrow have been demonstrated in normal pregnant mice, suggesting that B lymphopoiesis is sensitive to negative regulation by sex steroids [43,44]. The inhibitory effects of elevated estrogens suppress adult B-lymphopoiesis during pregnancy [45]. In animal models, progesterone represses the differentiation and maturity of B cells [46].…”

Section: Possible Mechanismsmentioning

confidence: 99%

Antibody Response to Pertussis Vaccination in Pregnant and Non-Pregnant Women—The Role of Sex Hormones

et al. 2021

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Pertussis containing vaccine is recommended for pregnant women to protect neonates prior to being fully immunized against the disease. The immune response during pregnancy may be impacted by changes in the hormonal status. The aim of this study was to evaluate the immune response to pertussis immunization in pregnancy and to assess the role of sex hormones. In a cross-sectional study, blood samples were drawn from 174 pregnant and 74 non-pregnant women 45–60 days following immunization. Anti-pertussis toxin (Anti-PT) IgG antibody levels, estrogen, and progestogen concentrations were compared between the two groups. Multiple logistic regression analysis was used to examine the association between serum antibody and sex hormone concentrations in each group, controlling for age, body mass index (BMI), and smoking status. The geometric mean concentration (GMC) of anti-PT IgG antibody was significantly higher in non-pregnant women compared with pregnant women (median of 2.09 and 1.86, interquartile range = 2.36–1.8 and 2.11–1.16 respectively, p < 0.0001). Among pregnant women, the anti-PT IgG antibody GMC was negatively associated with both progesterone (odds ratio = 0.300, 95% CI = 0.116, 0.772, p = 0.013) and estrogen (odds ratio = 0.071, 95% CI = 0.017, 0.292, p < 0.0001), after controlling for age, BMI, and smoking. Pregnancy was associated with lower anti-PT IgG antibody levels (odds ratio = 0.413, 95% CI = −0.190, 0.899, p = 0.026). This appears to be at least partially explained by the higher levels of hormones during pregnancy. These findings demonstrate the important role of sex hormones in the response to pertussis vaccine during pregnancy and can help to evaluate the optimum vaccination schedule.

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“…Interestingly, during fetal development, an intensive B-lymphopoiesis occurs in the middle- and late-stage FL despite high estrogen levels (12). Hlobeňová et al provided evidence that HSPCs are primed for estrogen sensitivity in FL at the E17–20 developmental stage, while the HSPCs from early E14 FL barely gain estrogen sensitivity after being transplanted into the adult BM microenvironment (34). Kinoshita et al have reported that primary culture of fetal hepatic cells from E14.5 murine embryos supported expansion of embryonic Lin – /Sca-1 + /c-Kit + (LSK) cells, giving rise to myeloid, lymphoid, and erythroid lineages (52).…”

Section: Emh Exhibits In the Embryo And Fetus During Developmentmentioning

confidence: 99%

Extramedullary Hematopoiesis (EMH) in Laboratory Animals: Offering an Insight into Stem Cell Research

et al. 2015

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Extramedullary hematopoiesis (EMH) is a pathological process secondary to underlying bone marrow (BM) insufficiency in adults. It is characterized by the emergence of multipotent hematopoietic progenitors scattered around the affected tissue, most likely in the spleen, liver, and lymph node, etc. EMH in patients frequently receives less medical attention and is neglected unless a compressive or obstructive hematopoietic mass appears to endanger the patient's life. However, on a biological basis, EMH reflects the alteration of relationships among hematopoietic stem and progenitor cells (HSPCs) and their original and new microenvironments. The ability of hematopoietic stem cells (HSCs) to mobilize from the bone marrow and to accommodate and function in extramedullary tissues is rather complicated and far from our current understanding. Fortunately, many reports from the studies of drugs and genetics using animals have incidentally found EMH to be involved. Thereby, the molecular basis of EMH could further be elucidated from those animals after cross-comparison. A deeper understanding of the extramedullary hematopoietic niche could help expand stem cells in vitro and establish a better treatment in patients for stem cell transplantation.

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B-lymphopoiesis gains sensitivity to subsequent inhibition by estrogens during final phase of fetal development

Cited by 6 publications

References 29 publications

T-lymphopoiesis is Severely Compromised in Ubiquitin-Green Fluorescent Protein Transgenic Mice

T-lymphopoiesis is Severely Compromised in Ubiquitin-Green Fluorescent Protein Transgenic Mice

Antibody Response to Pertussis Vaccination in Pregnant and Non-Pregnant Women—The Role of Sex Hormones

Extramedullary Hematopoiesis (EMH) in Laboratory Animals: Offering an Insight into Stem Cell Research

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