1996
DOI: 10.1046/j.1365-2958.1996.761428.x
|View full text |Cite
|
Sign up to set email alerts
|

Nucleotide sequence and role in DNA amplification of the direct repeats composing the amplifiable element AUD1 of Streptomyces lividans 66

Abstract: The amplifiable unit of DNA no. 1 (AUD1) of Streptomyces lividans consists of three 1 kb repeats (left direct repeat, LDR; middle direct repeat, MDR; and the slightly different right direct repeat, RDR) and two 4.7 kb repeats alternately arranged in identical orientation to each other. Both 4.7 kb repeats have been sequenced. They are identical and contain one open reading frame (orf4.7). The deduced amino acid sequence has a low similarity to chitinases, and two amino acid repeats present high similarities to… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

1
80
1

Year Published

1997
1997
2007
2007

Publication Types

Select...
9

Relationship

0
9

Authors

Journals

citations
Cited by 74 publications
(82 citation statements)
references
References 31 publications
1
80
1
Order By: Relevance
“…It exhibits 65% identity to ORF-Sl, the LacI/GalR-like protein of S. limosus (41a), and 55% identity to Streptomyces ORFs found in regions of chromosomal instability in S. ambofaciens and S. lividans (1,33,47). Reg1 has 31% identity with CcpA, the carbon catabolite repressor of Bacillus subtilis (16).…”
Section: Resultsmentioning
confidence: 99%
“…It exhibits 65% identity to ORF-Sl, the LacI/GalR-like protein of S. limosus (41a), and 55% identity to Streptomyces ORFs found in regions of chromosomal instability in S. ambofaciens and S. lividans (1,33,47). Reg1 has 31% identity with CcpA, the carbon catabolite repressor of Bacillus subtilis (16).…”
Section: Resultsmentioning
confidence: 99%
“…The RR Ͼ KK signal sequence mutation was introduced by QuikChange TM mutagenesis using the primer pair hip-KK-F (5Ј-C GAT AAG CCA ATC AGC AAG AGC AAG AAA GAC GCT GTC AAA GTG ATG-3Ј) and hip-KK-R (5Ј-CAT CAC TTT GAC AGC GTC TTT CTT GCT CTT GCT GAT TGG CTT ATC G-3Ј) with pEXH5-tac as a template, followed by the same cloning steps as described above, resulting in pEX-hip SP -KK-phoA-tac and pBADhip SP -KK-phoA. To express RR-phoA or KK-phoA under control of P rhaBAD , the NdeI/HindIII fragment of pEX-hip SP -phoA-tac was cloned into the corresponding sites of pJOE2702, which allows a rhamnoseregulated expression (22), resulting in pJOE-hip SP -phoA and pJOE-hip SP -KK-phoA. For coexpression of tatABC, the compatible low copy vector pABStatABC was used (17), which expresses tatABC under control of the tatA promoter and which confers chloramphenicol resistance.…”
Section: Methodsmentioning
confidence: 99%
“…The cloning vector pJOE2775, a pJOE2702 derivative plasmid (Volff et al, 1996), including the rhamnose-inducible E. coli promoter rhaB and a 3 sequence (GGATCC(CAT) 6 TGA) encoding a polyhistidine tag was kindly provided by Dr Anke Engels (University of Tübingen, Institute of Microbiology, Auf der Morgenstelle, Tübingen, Germany). Genomic yeast DNA was isolated by standard techniques (Burke et al, 2000).…”
Section: Plasmid and Dna Manipulationsmentioning
confidence: 99%