Phytochemical Screening and Evaluation of the Antiarthritic PotentialofAmmoides pusillaAqueous Extract on Freund's Adjuvant-Induced Rheumatoid Arthritis

Abstract

Background: Ammoides pusilla plant is a species of therapeutic interest which used in traditional medicines. This work aims to valorize this plant by the characterization of their bioactive components and the evaluation of the in vivo anti-inflammatory potential against a severe disease affecting the bone structure and the stability of the articular cartilage. Methods: First, the phytochemical screening of the polyphenolic extracts of A. pusilla was carried out. The second part of our study is devoted to the evaluation of the in vivo anti-inflammatory activity of the aqueous extract of A. pusilla based on the method of Freund's adjuvant-induced rheumatoid arthritis. Results: Phytochemical tests demonstrated the richness of extract with flavonoids, tannins, coumarins, anthocyanins and triterpenes. Whereas, the quantitative determination reveals that the aqueous extract of A. pusilla is the richest with bioactive components with contents of total polyphenols, flavonoids and tannins equal to 9.52 ± 0.11 mg gallic acid/g, 4.75 ± 0.05 mg quercetin/g and 8.64 ± 0.02 mg catechin/g respectively. The results of the anti-inflammatory activity showed that the aqueous infused extract of A. pusilla has an interesting antiarthritic potential on Freund's adjuvant-induced rheumatoid arthritis in mice. It is manifested by a weight gain; a normal arthritic index and biochemical parameters close to those of Diclofenac®. Conclusion: The aqueous infused extract of A. pusilla is therefore of considerable therapeutic interest as an alternative compound for the prevention of inflammation and for the improvement in bone structure.

Functionalization of Pasteurized Milk Using Rosemary, Thyme, and Ammoides Aqueous Extracts for Better Microbial Quality and an Improved Antioxidant Activity

“…The thyme TPC value ranges from 7.3 to 158-256 mg GAE/g, as reported by the literature [12,13]. As for ammoides extracts, the recorded TPC value was distinctly lower than the value reported by Belkhodja1 et al (2020) [11] in their study conducted on ammoides aqueous infusion. The different extracts have shown rich flavonoid content, representing more than 50% of the phenolic compounds.…”

“…Thyme and rosemary plants; their derivatives; and their pure compounds, such as carvacrol, thymol, and rosmarinic acid, have been used in the food industry to preserve and improve foods' sensory and functional properties [9,10]. Ammoides plants, on the other hand, culinary herbs that have been proven to be non-toxic and safe [11], have not yet been tested for potential applications in food.…”

Assessment of the antioxidant and nematicidal activities of an aqueous extract of Chromolaena odorata (L.) King and Robins against Radopholus similis infestation in Cavendish banana plants: An in vitro and in vivo study

“…Phytochemical quantification: Polyphenols, flavonoids, and tannins play pivotal roles in plant physiology, acting as potent antioxidants to shield against oxidative stress and demonstrating nematocidal properties, thereby fortifying plant defenses against nematode attacks. Precise quantification of these compounds in plant extracts is necessary, and it is executed employing well-established methodologies detailed by Belkhodja et al (2020). The determination of total polyphenol content involves the Folin-Ciocalteu method, expressing results as milligrams of gallic acid equivalent per gram of dry plant material (mg GAE/g) with the gallic acid standard.…”

Evaluation of Antioxidant Activity, Cytotoxicity, and Genotoxicity of Ptychotis verticillata Essential Oil: Towards Novel Breast Cancer Therapeutics

“…It is commonly employed in the treatment of digestive system disorders, particularly gastric and intestinal issues. Furthermore, it possesses analgesic, diuretic, and anti-asthmatic potentials [8]. Notably, P. verticillata is highly valued for its potent stimulating action, as well as its antioxidant and antimicrobial properties [5].…”