<b>Replacement of the lysine residue in the consensus ATP‐binding sequence of the AddA subunit of AddAB drastically affects chromosomal recombination in transformation and transduction of <i>Bacillus subtilis</i></b>

Haijema, Bert Jan; Noback, Michiel; Hesseling, Anne; Kooistra, J; Venema, Gerard; Meima, Rob

doi:10.1046/j.1365-2958.1996.601424.x

Cited by 20 publications

(30 citation statements)

References 31 publications

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“…As mentioned above, the AddAB helicase activity is conferred by the AddA subunit and has 3=-to-5= polarity (472,473). Inactivation of the ATP binding site (Walker A motif) in AddB has very little effect on the helicase activity of the AddAB complex, further demonstrating that AddA powers the helicase activity of AddAB (149)(150)(151). Unlike RecBCD, which contains two helicase activities and a single nuclease, AddAB has two nucleases that reside separately in its individual subunits, as well as a single helicase (for a review, see reference 471).…”

Section: End Processing By Addabmentioning

confidence: 84%

DNA Repair and Genome Maintenance in Bacillus subtilis

Lenhart

Schroeder

Walsh

et al. 2012

Microbiol Mol Biol Rev

151

155

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SUMMARY From microbes to multicellular eukaryotic organisms, all cells contain pathways responsible for genome maintenance. DNA replication allows for the faithful duplication of the genome, whereas DNA repair pathways preserve DNA integrity in response to damage originating from endogenous and exogenous sources. The basic pathways important for DNA replication and repair are often conserved throughout biology. In bacteria, high-fidelity repair is balanced with low-fidelity repair and mutagenesis. Such a balance is important for maintaining viability while providing an opportunity for the advantageous selection of mutations when faced with a changing environment. Over the last decade, studies of DNA repair pathways in bacteria have demonstrated considerable differences between Gram-positive and Gram-negative organisms. Here we review and discuss the DNA repair, genome maintenance, and DNA damage checkpoint pathways of the Gram-positive bacterium Bacillus subtilis. We present their molecular mechanisms and compare the functions and regulation of several pathways with known information on other organisms. We also discuss DNA repair during different growth phases and the developmental program of sporulation. In summary, we present a review of the function, regulation, and molecular mechanisms of DNA repair and mutagenesis in Gram-positive bacteria, with a strong emphasis on B. subtilis.

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Section: End Processing By Addabmentioning

confidence: 84%

DNA Repair and Genome Maintenance in Bacillus subtilis

Lenhart

Schroeder

Walsh

et al. 2012

Microbiol Mol Biol Rev

151

155

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“…This difference may suggest additional roles for Chi in H. influenzae. For example, RecBCD-mediated recombination in H. influenzae could also have a role in integration of incoming DNA during competence (as was reported for B. subtilis; Haijema et al, 1996). If this is the case, then integration of incoming DNA, regardless of its orientation, may be stimulated by Chi.…”

Section: Experimental Procedures)mentioning

confidence: 89%

Identification of the Chi site of Haemophilus influenzae as several sequences related to the Escherichia coli Chi site

Sourice

Biaudet

Karoui

et al. 1998

Molecular Microbiology

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SummaryThe Escherichia coli Chi site 5Ј-GCTGGTGG-3Ј modulates the activity of the powerful dsDNA exonuclease and helicase RecBCD. Genome sequence analyses revealed that Chi is frequent on the chromosome and oriented with respect to replication on the E. coli genome. Chi is also present much more frequently than predicted statistically for a random 8-mer sequence. Although it is assumed that Chi is ubiquitous, there is virtually no proof that its features are conserved in other microorganisms. We therefore identified and analysed the Chi sequence of an organism for which the full genome sequence was available, Haemophilus influenzae. The biological test we used is based on our finding that rolling circle plasmids provide a specific substrate for RecBCD analogues in different microorganisms. Unexpectedly, several related sequences, corresponding to 5Ј-GNTGGTGG-3Ј and 5Ј-G(G/C)TGGAGG-3Ј, showed Chi activity. As in E. coli, the H. influenzae Chi sites are frequent on the genome, which is in keeping with the need for frequent Chi sites for dsDNA break repair of chromosomal DNA. Although statistically over-represented, this feature is less marked than that of the E. coli Chi site. In contrast to E. coli, the H. influenzae Chi motifs are only slightly oriented with respect to the replication strand. Thus, although Chi appears to have a highly conserved biological role in attenuating exonuclease activity, its sequence characteristics and statistical representation on the genome may differ according to the particular features of the host.

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“…Nuclease and helicase domains were identified by comparing the amino acid sequences of H. pylori AddA and AddB with known motifs in E. coli RecB and RecD and in B. subtilis AddA and AddB (8,20,32,38,39,43,44). To inactivate the putative AddA helicase, we targeted the Walker A box responsible for ATP binding by changing codon 18 from Lys to Gln (K18Q), here designated AddA HEL .…”

Section: Construction Of Addab Nuclease and Helicase Mutants-tomentioning

confidence: 99%

Dual Nuclease and Helicase Activities of Helicobacter pylori AddAB Are Required for DNA Repair, Recombination, and Mouse Infectivity

Amundsen

Fero

Salama

et al. 2009

Journal of Biological Chemistry

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Helicobacter pylori infection of the human stomach is associated with disease-causing inflammation that elicits DNA damage in both bacterial and host cells. NUC showed partial attenuation. E. coli ⌬recBCD expressing H. pylori addAB was recombination-deficient unless H. pylori recA was also expressed, suggesting a species-specific interaction between AddAB and RecA and also that H. pylori AddAB participates in both DNA repair and recombination. These results support a role for both the AddAB nuclease and helicase in DNA repair and promoting infectivity.

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Replacement of the lysine residue in the consensus ATP‐binding sequence of the AddA subunit of AddAB drastically affects chromosomal recombination in transformation and transduction of Bacillus subtilis

Cited by 20 publications

References 31 publications

DNA Repair and Genome Maintenance in Bacillus subtilis

DNA Repair and Genome Maintenance in Bacillus subtilis

Identification of the Chi site of Haemophilus influenzae as several sequences related to the Escherichia coli Chi site

Dual Nuclease and Helicase Activities of Helicobacter pylori AddAB Are Required for DNA Repair, Recombination, and Mouse Infectivity

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