<b>Replication terminus for DNA polymerase I during initiation of pAMβ1 replication: role of the plasmid‐encoded resolution system</b>

Janniere, Laurent; Bidnenko, Vladimir; McGovern, Stephen; Ehrlich, S. Dusko; Petit, Marie‐Agnès

doi:10.1046/j.1365-2958.1997.d01-1874.x

Cited by 23 publications

(29 citation statements)

References 36 publications

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“…Bruand, personal communication). Initiation occurs at a single position within ori and progresses unidirectionally generating intermediates carrying a D-loop structure (Bruand et al, 1993;Le Chatelier et al, 1993;Janniè re et al, 1997). D-loop extension is arrested Ϸ200 bp downstream from ori by two independent and additive mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of a naturally occurring rolling‐circle replicon in derivatives of the theta‐replicating plasmid pIP501

Pujol

Chédin

Ehrlich

et al. 1998

Molecular Microbiology

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SummaryThe mechanisms ensuring regulation of DNA replication in genomes containing multiple replicons are poorly understood. In this report, we addressed this question by analysing in Bacillus subtilis the replication of a derivative of the promiscuous plasmid pIP501 that carries a rolling-circle and a theta replicon. Genetic analyses revealed that the rolling-circle replicon is strongly inhibited in the derivative and that inhibition requires three elements involved in theta replication: the replication origin, the initiator RepR protein and strong transcription of the repR gene. Inhibition is, however, independent of DNA synthesis at the theta origin. We conclude that rolling-circle inhibition is caused by an inhibitor y signal encoded by the theta replicon and propose that the signal is composed, at least, of the RepR protein bound to its cognate origin.

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Section: Introductionmentioning

confidence: 99%

Inhibition of a naturally occurring rolling‐circle replicon in derivatives of the theta‐replicating plasmid pIP501

Pujol

Chédin

Ehrlich

et al. 1998

Molecular Microbiology

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“…The resolvase gene found in pUE10 in Deinococcus radiodurans SARK was reported directly to affect its stability under non-selective conditions, but not its transformation efficiency. 16) But in the cases of pSM19035 in Streptococcus pyogenes 19) and pAM1 in Enterococcus faecalis, 20) the recombinases are required also for plasmid replication. Thus it is necessary to investigate further the necessity of resU in pSM103mini replication and maintenance.…”

Section: Determination Of Indispensable Regions For Plasmid Maintenancementioning

confidence: 99%

Characterization of a Cryptic Plasmid, pSM103mini, from Polyethylene-Glycol DegradingSphingopyxis macrogoltabidaStrain 103

Tani

Tanaka

Minami

et al. 2011

Bioscience, Biotechnology, and Biochemistry

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“…Functional analysis of resU, the putative resolvase gene. Although the mechanisms underlying the stabilizing effect of plasmid-encoded site-specific recombinases on plasmid maintenance are not fully understood, their role has been suggested in several studies (8,19,30,33,38). It has long been established that these so-called resolvases contribute to plasmid maintenance by resolving plasmid multimers to monomers, thus increasing the number of segregation units to be distributed during cell division (for a review, see reference 30).…”

mentioning

confidence: 99%

Characterization of the Minimal Replicon of a Cryptic Deinococcus radiodurans SARK Plasmid and Development of Versatile Escherichia coli-D. radiodurans Shuttle Vectors

Meima¹,

Lidstrom

2000

Appl Environ Microbiol

100

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The nucleotide sequence of a 12-kb fragment of the cryptic Deinococcus radiodurans SARK plasmid pUE10 was determined, in order to direct the development of small, versatile cloning systems for Deinococcus. Annotation of the sequence revealed 12 possible open reading frames. Among these are the repU and resU genes, the predicted products of which share similarity with replication proteins and site-specific resolvases, respectively. The products of both genes were demonstrated using an overexpression system in Escherichia coli. RepU was found to be required for replication, and ResU was found to be required for stable maintenance of pUE10 derivatives. Gel shift analysis using purified His-tagged RepU identified putative binding sites and suggested that RepU may be involved in both replication initiation and autoregulation of repU expression. In addition, a gene encoding a possible antirestriction protein was found, which was shown to be required for high transformation frequencies. The arrangement of the replication region and putative replication genes for this plasmid from D. radiodurans strain SARK is similar to that for plasmids found in Thermus but not to that for the 45.7-kb plasmid found in D. radiodurans strain R1. The minimal region required for autonomous replication in D. radiodurans was determined by sequential deletion of segments from the 12-kb fragment. The resulting minimal replicon, which consists of approximately 2.6 kb, was used for the construction of a shuttle vector for E. coli and D. radiodurans. This vector, pRAD1, is a convenient general-purpose cloning vector. In addition, pRAD1 was used to generate a promoter probe vector, and a plasmid containing lacZ and a Deinococcus promoter was shown to efficiently express LacZ.Ever since its discovery in 1956 (3), Deinococcus radiodurans and other members of the Deinococcaceae have become the paradigm of natural resistance to high-level ionizing and UV radiation. D. radiodurans cells are able to survive levels of gamma radiation 1,000 times higher than the lethal dose for humans and were shown to survive and accurately repair massive DNA damage. At 1.5 megarads, up to 130 double-strand breaks occur per cell, which are repaired without mutagenesis or loss of viability (13). In contrast, the presence of a mere two double-strand breaks is lethal to Escherichia coli (21). As a consequence, this pink-pigmented, non-spore-forming bacterium has received considerable attention from the scientific community, from both a fundamental and an applied point of view. Because of their radioresistance, Deinococcus species hold great potential for bioremediation of complex waste mixtures containing organic solvents, heavy metals, and radioisotopes. Recently, Lange et al. (23) have demonstrated toluene dioxygenase (TDO) activity in engineered strains of D. radiodurans R1. TDO is a broad-spectrum dioxygenase capable of degrading trichloroethylene (42), a common organopollutant at many Department of Energy waste sites (32). In these strains, TDO activity was not inhibi...

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Replication terminus for DNA polymerase I during initiation of pAMβ1 replication: role of the plasmid‐encoded resolution system

Cited by 23 publications

References 36 publications

Inhibition of a naturally occurring rolling‐circle replicon in derivatives of the theta‐replicating plasmid pIP501

Inhibition of a naturally occurring rolling‐circle replicon in derivatives of the theta‐replicating plasmid pIP501

Characterization of a Cryptic Plasmid, pSM103mini, from Polyethylene-Glycol DegradingSphingopyxis macrogoltabidaStrain 103

Characterization of the Minimal Replicon of a Cryptic Deinococcus radiodurans SARK Plasmid and Development of Versatile Escherichia coli-D. radiodurans Shuttle Vectors

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