<b>Sensing of DNA non‐homology lowers the initiation of meiotic recomination in yeast</b>

Rocco, Vincenzo; Nicolas, Alain

doi:10.1046/j.1365-2443.1996.00256.x

Cited by 49 publications

(29 citation statements)

References 60 publications

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“…For a meiotic DSB to function efficiently as a recombination initiator, i.e., for a heteroduplex to form, the allelic sequence must be present in the homolog. Thus, promoter deletions, even if heterozygous, should interfere with the initiation of recombination, as they do in yeast, where they lower both DSB formation and the frequency of conversion of a downstream marker (33). We found that the bz-s39.8 deletion, which includes the bz promoter, caused a major reduction in the conversion frequency of heteroalleles on the 5′, but not the 3′, half of the gene (Fig.…”

Section: Discussionmentioning

confidence: 62%

Polarized gene conversion at the bz locus of maize

Dooner

2014

Proc. Natl. Acad. Sci. U.S.A.

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Nucleotide diversity is greater in maize than in most organisms studied to date, so allelic pairs in a hybrid tend to be highly polymorphic. Most recombination events between such pairs of maize polymorphic alleles are crossovers. However, intragenic recombination events not associated with flanking marker exchange, corresponding to noncrossover gene conversions, predominate between alleles derived from the same progenitor. In these dimorphic heterozygotes, the two alleles differ only at the two mutant sites between which recombination is being measured. To investigate whether gene conversion at the bz locus is polarized, two large diallel crossing matrices involving mutant sites spread across the bz gene were performed and more than 2,500 intragenic recombinants were scored. In both diallels, around 90% of recombinants could be accounted for by gene conversion. Furthermore, conversion exhibited a striking polarity, with sites located within 150 bp of the start and stop codons converting more frequently than sites located in the middle of the gene. The implications of these findings are discussed with reference to recent data from genome-wide studies in other plants.meiotic recombination | conversion polarity

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Section: Discussionmentioning

confidence: 62%

Polarized gene conversion at the bz locus of maize

Dooner

2014

Proc. Natl. Acad. Sci. U.S.A.

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“…Additional evidence for interactions between homologs that are independent of recombination comes from studies of DSB induction in diploids that are either homozygous or heterozygous at the DSB site. Heterozygosity decreases the frequency of DSBs, suggesting that homolog recognition precedes and promotes the initiation of recombination Rocco and Nicolas 1996).…”

Section: Is Recombination Required For Pairing?mentioning

confidence: 99%

Meiotic chromosomes: it takes two to tango

Gs¹

1997

Genes Dev.

783

656

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“…FISH studies of homolog pairing indicate that at least some homolog recognition occurs in the absence of DSBs (Loidl et al 1994;Weiner and Kleckner 1994;Nag et al 1995;Rockmill et al 1995). In studies of DSB induction, the level of breakage on one chromosome was found to be affected by sequences present on the homolog, suggesting that homologous chromosomes normally associate with each other prior to the formation of DSBs (Xu and Kleckner 1995;Bullard et al 1996;Rocco and Nicolas 1996). Timecourse analyses indicate that DSBs are formed early in meiotic prophase prior to the initiation of SC formation (Padmore et al 1991).…”

Section: How Do Telomeres Promote Pairing?mentioning

confidence: 99%

Telomere-mediated chromosome pairing during meiosis in budding yeast

Rockmill¹,

Roeder²

1998

Genes Dev.

123

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Certain haploid strains of Saccharomyces cerevisiae can undergo meiosis, but meiotic prophase progression and subsequent nuclear division are delayed if these haploids carry an extra chromosome (i.e., are disomic). Observations indicate that interactions between homologous chromosomes cause a delay in meiotic prophase, perhaps to allow time for interhomolog interactions to be completed. Analysis of meiotic mutants demonstrates that the relevant aspect of homolog recognition is independent of meiotic recombination and synaptonemal complex formation. A disome in which the extra chromosome is circular sporulates without a delay, indicating that telomeres are important for homolog recognition. Consistent with this hypothesis, fluorescent in situ hybridization demonstrates that a circular chromosome has a reduced capacity to pair with its homolog, and a telomere-associated meiotic protein (Ndj1) is required to delay sporulation in disomes. A circular dimer containing two copies of the same chromosome delays meiosis to the same extent as two linear homologs, implying that physical proximity bypasses the requirement for telomeres in homolog pairing. Analysis of a disome carrying two linear permuted chromosomes suggests that even nonhomologous chromosome ends can promote homolog pairing to a limited extent. We speculate that telomere-mediated chromosome movement and/or telomere clustering promote homolog pairing.

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Sensing of DNA non‐homology lowers the initiation of meiotic recomination in yeast

Cited by 49 publications

References 60 publications

Polarized gene conversion at the bz locus of maize

Polarized gene conversion at the bz locus of maize

Meiotic chromosomes: it takes two to tango

Telomere-mediated chromosome pairing during meiosis in budding yeast

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