The Anti-inflammatory Potential of a Strain of Probiotic Bifidobacterium pseudocatenulatum G7: In Vitro and In Vivo Evidence

Abstract

The genus Bifidobacterium has been widely used in functional foods for health promotion due to its beneficial effects on human health, especially in the gastrointestinal tract (GIT). In this study, we characterize the anti-inflammatory potential of the probiotic strain Bifidobacterium pseudocatenulatum G7, isolated from a healthy male adult. G7 secretion inhibited inflammatory response in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Moreover, oral administration of bacteria G7 alleviated the severity of colonic inflammation in dextran sulfate sodium (DSS)-treated colitis mice, which was evidenced by a decreased disease activity index (DAI) and enhanced structural integrity of the colon. The 16S rRNA gene sequencing result illustrated that the G7 alleviated DSS-induced gut microbiota dysbiosis, accompanied by the modulated bile acids and short-chain fatty acid (SCFA) levels. Overall, our results demonstrated the potential anti-inflammatory effects of Bifidobacterium pseudocatenulatum G7 on both in vitro and in vivo models, which provided a solid foundation for further development of a novel anti-inflammatory probiotic.

Bifidobacterium bifidum Ameliorates DSS-Induced Colitis in Mice by Regulating Microbial Metabolome and Targeting Gut Microbiota

“…Lactobacillus rhamnosus CY12 has been demonstrated to ameliorate colitis through modulation of the gut microbiota, restoration of intestinal barrier function, and suppression of the TLR4-MyD88-NF-κB pathway Bifidobacterium pseudocatenulatum G7 has been found to alleviate colitis by addressing gut microbiota dysbiosis and regulating bile acid and short-chain fatty acid (SCFA) levels . Moreover, Lactiplantibacillus plantarum AS21 has shown promise in improving DSS-induced colitis by modulating the gut microbiota, enhancing intestinal function, and inhibiting the p38-MAPK/NF-κB pathway in colonic tissues, thereby mitigating inflammation and oxidative stress .…”