The Effects of Hydroalcoholic Extract ofArum orientaleon CLP-Induced Sepsis in Rats

Abstract

Background: Sepsis is a lethal clinical syndrome that results from dysregulated systemic inflammatory response of the body due to the invasion of pathogens, especially bacteria. Despite advances in medical care and therapy, sepsis is still one of the major causes of death in intensive care units and no decisive medical treatment is available against that. Studies have suggested that some Arum species have anti-bacterial properties. The present study investigated the effects of hydroalcoholic extract of Arum orientale, on cecal ligation and puncture (CLP) induced sepsis in rats. Methods: CLP method was used for induction of sepsis in rats. Hydroalcoholic extract of A. orientale was injected intraperitoneally with doses of 80 and 640 mg/Kg body weight at times of 0, 1, 3, 6 and 24 h after the surgery. Antibacterial activity, hemodynamic parameters, myeloperoxidase (MPO) activity and survival rate were measured after 72 h. Results: Hydroalcoholic extract of A. orientale showed antibacterial activities as potent as gentamycin against Escherichia coli. Administration of the extract with a dose of 80 mg/Kg body weight increased significantly hemodynamic parameters such as mean arterial pressure (p<0.05)and decreased optical density (OD) (p<0.05) of blood. The extract also increased serum MPO activity (p<0.01) and reduced survival rate to 20%. Conclusion: This study for the first time showed that hydroalcoholic extract of A. oriental acts as a double edge sword in the treatment of CLP-induced sepsis. This extract showed antibacterial properties and also improved hemodynamic parameters but decreased survival rate, that might be through pro-inflammatory effects.