B-type natriuretic peptide and N-terminal pro B-type natriuretic peptide are comparably useful for disease monitoring in heart failure

Hammerer-Lercher, Angelika; Pölzl, Gerhard; Falkensammer, Gerda; Ludwig, Wilma; Hügel, Heike; Puschendorf, Bernd; Pachinger, Otmar; Mair, Johannes

doi:10.1016/j.ijcard.2005.01.016

Cited by 10 publications

(6 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Natriuretic peptides are helpful for guidance of therapy and monitoring disease course in HF patients and for risk stratification (Hammerer-Lercher et al 2006). Studies have also shown that high plasma hs-CRP levels predicted poor long-term outcomes in patients with HF (Tang et al 2008).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The inflammatory biomarker YKL-40 as a new prognostic marker for all-cause mortality in patients with heart failure

Harutyunyan,

Christiansen,

Johansen

et al. 2012

Immunobiology

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

“…have a poor prognosis, and the morbidity and mortality in these patients remain high. The biomarkers N-terminal-pro-B natriuretic peptide (NT-proBNP) and high-sensitivity C-reactive protein (hs-CRP) have been the focus for monitoring and risk assessment in patients with HF (Hammerer-Lercher et al 2006;Scirica et al 2009;Tang et al 2008).…”

Section: Introductionmentioning

confidence: 99%

The inflammatory biomarker YKL-40 as a new prognostic marker for all-cause mortality in patients with heart failure

Harutyunyan,

Christiansen,

Johansen

et al. 2012

Immunobiology

View full text Add to dashboard Cite

“…Elevated serum levels of brain natriuretic peptide (BNP) [13][14][15][16][17][18][19][20][21] are also associated with poor prognosis in patients with PH [22,23], which is greatly influenced by volume overload, age, and renal function [24]. Here, we evaluated RA contractility and RV diastolic function in adult subjects with acquired chronic PH and normal electrocardiographic sinus rhythms by pulsed TDI and assessed their relationship with serum BNP using multiple regression analysis.…”

Section: Introductionmentioning

confidence: 99%

Right atrium contractility and right ventricular diastolic function assessed by pulsed Tissue Doppler Imaging can predict brain natriuretic peptide in adults with acquired pulmonary hypertension

Shiina

Funabashi

Lee

et al. 2009

International Journal of Cardiology

View full text Add to dashboard Cite

“…BNP regulates cardiovascular homeostasis and has been used as drug treatment for congestive heart failure in acutely decompensated patients [3]. Moreover, clinical immunoassays for both BNP and NT-proBNP have been FDA-approved for the diagnosis of left ventricle dysfunction [4][5][6].…”

mentioning

confidence: 99%

A glycosylated form of the human cardiac hormone pro B-type natriuretic peptide is an intrinsically unstructured monomeric protein

Crimmins

Kao

2008

Archives of Biochemistry and Biophysics

View full text Add to dashboard Cite

The N-terminal fragment of pro B-type natriuretic peptide (NT-proBNP) and proBNP are used as gold standard clinical markers of myocardial dysfunction such as cardiac hypertrophy and left ventricle heart failure. The actual circulating molecular forms of these peptides have been the subject of intense investigation particularly since these analytes are measured in clinical assays. Conflicting data has been reported and no firm consensus on the exact nature of the molecular species exists. Because these clinical assays are immunoassay-based, specific epitopes are detected. It is conceivable then that certain epitopes may be masked and therefore unavailable for antibody binding, thus the importance of determining the nature of the circulating molecular forms of these analytes. This situation is an unavoidable Achilles' heel of immunoassays in general.A recombinant O-linked glycosylated form of proBNP has been show to mimic some of the properties of extracted plasma from a heart failure patient. In particular the recombinant and native material co-migrated as diffuse Western-immunostained bands on SDS-PAGE and each band collapsed to an apparent homogeneous band following deglycosylation. Thus, glycosylated-proBNP may be one such circulating form. Here we provide extensive physiochemical characterization for this O-linked protein and compare these results to other described circulating species, non-glycosylated-proBNP and NT-proBNP. It will be shown that glycosylation has no influence on the secondary and quaternary structure of proBNP. In fact, at moderate concentration in benign physiological neutral pH buffer, all three likely circulating species are essentially devoid of major secondary structure, i.e., are intrinsically unstructured proteins (IUPs). Furthermore, all three proteins exist as monomers in solution. These results may have important implications in the design of NT-proBNP/BNP immunoassays. Immunoassays utilize antibodies that react with specific analyte (usually protein) epitope(s). If these epitopes become unreactive due to say proteolytic processing, or masked by interaction with other molecule(s), or are post-or co-translationally modified, then the immunoassay can generate incorrect results. It becomes important then to identify the circulating forms of the analytes. This analysis can include primary sequence, post-translational modifications, and quaternary structure as homo-and hetero-interaction partners. Knowledge of such can aid in the development of immunoassays that accurately detect the circulating species. Keywords GlycosylatedPrevious characterization of human NT-proBNP whether as a recombinant product or synthetic peptide showed this N-terminal fragment to be a largely unfolded, flexible protein, termed an IUP, which exists as a monomer in solution [7,8]. No comparable data were hitherto available for proBNP and a recently discovered [9] glycosylated form of proBNP. Here we use a combination of CD to assess the global average secondary structure of proBNP and glycosylated-proBNP...

show abstract

B-type natriuretic peptide and N-terminal pro B-type natriuretic peptide are comparably useful for disease monitoring in heart failure

Cited by 10 publications

References 9 publications

The inflammatory biomarker YKL-40 as a new prognostic marker for all-cause mortality in patients with heart failure

The inflammatory biomarker YKL-40 as a new prognostic marker for all-cause mortality in patients with heart failure

Right atrium contractility and right ventricular diastolic function assessed by pulsed Tissue Doppler Imaging can predict brain natriuretic peptide in adults with acquired pulmonary hypertension

A glycosylated form of the human cardiac hormone pro B-type natriuretic peptide is an intrinsically unstructured monomeric protein

Contact Info

Product

Resources

About