B vitamins induce an antinociceptive effect in the acetic acid and formaldehyde models of nociception in mice

França, D S; Souza, Adriano Lopes de; Almeida, Karine R; Dolabella, Silvio Santana; Martinelli, Celso; Coelho, Márcio M.

doi:10.1016/s0014-2999(01)01038-x

Cited by 125 publications

(120 citation statements)

References 26 publications

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“…Similar trend of findings has been observed by Franca et al 16 , though they investigated the effect of administration of individual thiamine as well as combined (20:20:1) thiamine, pyridoxine and cyanocobalamine (TPC), both at 50 and 100 mg/ kg (i.p.). Furthermore decrement of pain response was also reported by other researchers after single oral administration of TPC at different doses in different pain models 13,18,19 .…”

Section: Discussionsupporting

confidence: 83%

“…Formalin induced paw edema test 16,31,39 : Anti-inflammatory effect of thiamine was determined by the formalin induced paw edema model, where the amount of paw edema resulted from intra-planter injection of 50 µl of 2.5% formalin was used an indicator of inflammation severity. Following 60 minutes of recording the pain behaviors (about 120 minutes after the i.p administration of THCL or NS), the animal was sacrificed.…”

Section: Methodsmentioning

confidence: 99%

“…In addition, 125 mg/kg/body weight of thiamine decreased acute inflammation in the animals' ear induced by xylene 21 . However, Franca et al have shown that, thiamine at chronic (for ³ 7consecutive days administration) doses with 50 and 100 mg/kg/ body weight, reduced pain in the second phase (16th to 30th minutes) of formalin test 16 . Similar trend of decrement in the inflammatory pain after administration of different doses of combination of thiamine, B6 and B12 have been reported in several studies 13,15,17,18 .…”

mentioning

confidence: 99%

“…Several studies have shown that high doses of thiamine with vitamin B 6 and B 12 , potentiate the antinociceptive effect of diclofenac, ketorolac, ketoprofen as well as acetaminophen [12][13][14][15] . Furthermore, in several animal studies combined thiamine, B6 and B12 have been found effective to reduce nociceptive and inflammatory pain, both after single (acute) as well as after chronic administrations (for ³ 7consecutive days) 9,13,[15][16][17][18][19] .…”

mentioning

confidence: 99%

“…Therefore, this study was designed to evaluate the antinociceptive as well as anti-inflammatory effects of chronic (single daily dose for 7 consecutive days) supplementations of thiamine in increasing doses (100, 200 and 250 mg/kg of body weight) by formalin test and formalin induced paw edema test, in rats. 16,20,22,30,[37][38] : The rats were acclimatized in the experimental laboratory environment, in the observation cage of the plexiglass formalin box (30×30×30 cm3) for 1 hour daily (within 8:00 to 14:00 hours) for 7 consecutive days prior to the formalin test. Then on the day of experiment (Day 7), 1 hour after last dose of (i.p.)…”

mentioning

confidence: 99%

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Antinociceptive and anti-inflammatory effects of thiamine in Long Evans rats

Ainan

Begum

Ali

2016

J Bangladesh Soc Physiol

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Background: Thiamine along with other B vitamins has been prescribed since long for treatment of various painful conditions, though individual effects of thiamine on nociception and inflammation are yet to be clearly demonstrated. Objective: To assess the effects of increasing doses of thiamine supplementation against pain and inflammation. Methods: This experimental study was conducted in the Department of Physiology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka from March 2014 to February 2015. Total twenty four male Long Evans rats weighing (200±20 gm) were treated with three different doses of thiamine hydrochloride (THCL 100, 200, 250, mg/kg/day; experimental) or normal saline (5 ml/kg/day; control) intraperitoneally for 7 consecutive days. To evaluate the thiamine's effect on nociceptive pain, early phase (0-5 minutes) and on inflammatory pain, late phase (16-60 minutes) of the formalin test, were observed. In both phases, total frequency of jerking and total duration of flexing and licking of the right hind paw were counted after administration of subcutaneous formalin (50 µl, 2.5%) injection. After formalin test, all the rats were also subjected to formalin induced paw edema test using a water plethysmometer to observe the antiinflammatory effect of thiamine. Statistical analysis was done by ANOVA, followed by Bonferroni post hoc test. In the interpretation of results, p≤0.05 was considered as significant. Results: In formalin test, thiamine lowered frequency of jerking (after all 3 doses, in both phases) and duration of flexing and licking (200 mg/kg -p<0.001, in late phases; 250 mg/kg (p<0.001), in both phases) significantly. Additionally, thiamine lowered paw edema significantly (p<0.001) in higher 2 doses. Conclusion: This study concludes that, thiamine may have dose dependent antinociceptive and anti-inflammatory effects.

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Section: Discussionsupporting

confidence: 83%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Antinociceptive and anti-inflammatory effects of thiamine in Long Evans rats

Ainan

Begum

Ali

2016

J Bangladesh Soc Physiol

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Antinociceptive and antiinflammatory effects of ketoprofen are potentiated by a vitamin B mixture in the rat

Rodrı́guez-Silverio

Aguilar-Carrasco

Reyes-Garcı́a

et al. 2005

Drug Development Research

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Ketoprofen is a nonsteroidal antiinflammatory drug (NSAID) widely used in therapeutics. However, gastrointestinal toxicity may limit its use. To reduce the requirements of NSAIDs and hence their toxicity, these compounds have been combined with several drugs. B vitamins increase the analgesic activity of NSAIDs, but conflicting results have been obtained. The purpose of this study was to establish whether a vitamin B mixture can increase the antinociceptive effect of ketoprofen in the thermal paw stimulation model and in antiinflammatory activity in carrageenan-induced paw edema in the rat. Ketoprofen produced a dose-dependent antinociception, whereas a mixture of B vitamins produced a very limited antinociceptive effect. However, B vitamins were able to increase the antinociceptive effect of ketoprofen, reaching effects similar to those obtained with the maximal dose of ketoprofen tested. Ketoprofen produced a dose-dependent antiinflammatory activity and B vitamins also produced significant antiinflammatory effects. When ketoprofen was combined with a mixture of B vitamins, an increase in the antiinflammatory action was observed. These results indicate that coadministration of B vitamins with ketoprofen can increase both antihyperalgesic and antiinflammatory effects. Thus, B vitamins are effective as analgesic adjuvants in the models tested. Drug Dev. Res. 64:66-70, 2005.

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Synergistic antinociceptive interaction between acetaminophen or metamizol and B vitamins in the formalin test

et al. 2005

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There is evidence that B vitamins produce antinociception in animals. However, potentiation of NSAID-induced antinociception by B vitamins is unclear. The current study was designed to investigate the antinociceptive interaction between a mixture of B vitamins and either acetaminophen or metamizol. Acetaminophen (56-316 mg/kg), metamizol (32-178 mg/kg), and the mixture of B vitamins (32-178 mg/kg of thiamine, pyridoxine, and cyanocobalamin in a 100:100:1 proportion, respectively) or a combination of each drug with the B vitamins mixture was administered orally to female Wistar rats, and the antinociceptive effect determined in the formalin test. Isobolographic analyses were used to define the nature of the interaction between NSAIDs and B vitamins. Oral administration of either drug produced a dose-related antinociceptive effect. Isobolographic analyses revealed that both acetaminophen or metamizol and the B vitamins mixture interacted synergistically in the formalin test, suggesting that these two combinations could be useful in treating inflammatory pain states. Drug Dev. Res. 66:286-294, 2006.

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B vitamins induce an antinociceptive effect in the acetic acid and formaldehyde models of nociception in mice

Cited by 125 publications

References 26 publications

Antinociceptive and anti-inflammatory effects of thiamine in Long Evans rats

Antinociceptive and anti-inflammatory effects of thiamine in Long Evans rats

Antinociceptive and antiinflammatory effects of ketoprofen are potentiated by a vitamin B mixture in the rat

Synergistic antinociceptive interaction between acetaminophen or metamizol and B vitamins in the formalin test

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