B6D2F1 mice that retain sexual behavior long term after castration outperform those that cease in the radial arm maze

Neuron- and microglia-specific immunoexpression in steroid-independent male sexual behaviour in castrated B6D2F1 male mice

“…On top of that, one notable difference was that the mice in our study were 17 -18 months old on their last MSB test, which is older than the traditional breeding age (Moreira et al, 2015). Comparatively, the mice in previous studies were around 4 -6 months of age on their last MSB test David, Wyrosdic, Wan, et al, 2022). This is a potential confound as ageing is known to impair MSB (Wilson et al, 2018;Jesús et al, 2022).…”

“…However, after they were provided social exposure, 20.59% of the males demonstrated consistent SIMSB at least 62 weeks post-orchidectomy. Because this current study differed from the previous SIMSB paradigms in our lab, we refer to the males that demonstrated SIMSB in this study as 'SI persistent maters' and those that did not as 'SI non-maters' to differentiate these mice from prior studies David, Wyrosdic, Wan, et al, 2022).…”

“…However, after they were provided social exposure, 20.59% of the males demonstrated consistent SIMSB at least 62 weeks post-orchidectomy. Because this current study differed from the previous SIMSB paradigms in our lab, we refer to the males that demonstrated SIMSB in this study as ‘SI persistent maters’ and those that did not as ‘SI non-maters’ to differentiate these mice from prior studies (David, Wyrosdic, & Park, 2022; David, Wyrosdic, Wan, et al ., 2022). Once SIMSB was reinstated, we then set out to test our original experiment which was to investigate neuron and microglia-specific immunoexpression using flow cytometry in the POA.…”