Babesia canis infection in canine-red blood cell-substituted SCID mice

Arai, Satoru; Tsuji, Masayoshi; Kim, Sam-Ju; Nakade, Tetsuya; Kanno, Yasunori; Ishihara, Chiaki

doi:10.1016/s0020-7519(98)00094-0

Cited by 16 publications

(15 citation statements)

References 12 publications

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“…Hence, despite the apparent absence of U.S.-type B. microti in Japan, it may be possible to find this type in that region of the country. A sample from Citellus erythrogenys trapped in Xinjiang, China, contained a Babesia parasite whose rDNA was the most closely related to that of B. canis vogeli isolated from a dog in Okinawa Japan [1]. Whether this parasite is involved in any disease of dogs or humans is currently unknown, although a human case that was suggested to be due to B. canis has been reported [23].…”

Section: Discussionmentioning

confidence: 99%

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Epizootiologic Survey for <i>Babesia microti</i> among Small Wild Mammals in Northeastern Eurasia and a Geographic Deversity in the β-Tubulin Gene Sequences

Zamoto

Tsuji

Wang

et al. 2004

The Journal of Veterinary Medical Science

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103

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ABSTRACT. We previously reported that small wild rodents in Japan harbor two types of novel Babesia microti-like parasites (designated as Hobetsu and Kobe types), but not the type commonly found in the northeastern United States (U.S. type) where human babesiosis is endemic. To determine whether these new types of parasites are distributed in places surrounding Japan, an epizootiologic survey was undertaken in three geographically distant areas in northeastern Eurasia; South Korea, Vladivostok in Russia, and Xinjiang in China. Blood samples were collected from a total of 387 animals comprising 24 species. DNAs extracted from the samples were tested by nested PCR targeting babesial nuclear small-subunit rRNA gene (rDNA), which revealed that small rodents harboring B. microti exist in all three survey areas. Sequence analysis showed that all PCR-positive samples had rDNA sequences virtually identical to that of U.S.-type B. microti. However, when β-tubulin gene sequences were compared, evident geographic variations were seen. By use of primers specific for each of the β-tubulin genes of Kobe-, Hobetsu-, and U.S.-type parasites, a type-specific PCR was developed. Parasite with Hobetsu-or Kobe-type sequence was not detected from any of the three survey areas. These findings suggest that U.S.-type B. microti is widely distributed among small wild mammals in temperate zones of not only North America, but also Eurasia, whereas that Hobetsu-and Kobe-type parasites may be uniquely distributed in Japan.

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Section: Discussionmentioning

confidence: 99%

“…The Australian strain of B. rodhaini, kindly provided by the National Institute of Animal Health, Tsukuba, Japan, was propagated in BALB/c mice. Babesia canis vogeli, Okinawa isolate, has been described in an earlier study [1].…”

Section: Methodsmentioning

confidence: 97%

Epizootiologic Survey for <i>Babesia microti</i> among Small Wild Mammals in Northeastern Eurasia and a Geographic Deversity in the β-Tubulin Gene Sequences

Zamoto

Tsuji

Wang

et al. 2004

The Journal of Veterinary Medical Science

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103

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“…DMA in 0.5 ml PBS was administered to the control group. Clindamycin (Dalacin; Upjohn Ltd.) at a dosage of 500 mg/kg was administered intraperitoneally in 0.5 ml PBS to the second experimental group (6). Diminazene aceturate (Ganaseg; Japan CiebaGeigy Ltd.) was administrated subcutaneously at a dosage of 25 mg/kg in 0.15 ml double-distilled water to the third experimental group (4).…”

Section: Methodsmentioning

confidence: 99%

Apicoplast-Targeting Antibacterials Inhibit the Growth of Babesia Parasites

AbouLaila

Munkhjargal

Sivakumar

et al. 2012

Antimicrob Agents Chemother

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“…Isolates from other piroplasms: The isolates from piroplasms outside the B. microti group (Table 1) [2,12,33,36]. In addition, B. gibsoni and T. sergenti Ikeda were provided by Yoshimitsu Maede and Misao Onuma (Hokkaido University, Japan), respectively.…”

Section: Methodsmentioning

confidence: 99%

“…In addition, B. gibsoni and T. sergenti Ikeda were provided by Yoshimitsu Maede and Misao Onuma (Hokkaido University, Japan), respectively. These 6 isolates were propagated in a SCID mouse model substituting the red blood cells with either bovine or canine erythrocytes as a precaution to guard against possible formation of PCR chimeras by DNA strands from the different species [2,36].…”

Section: Methodsmentioning

confidence: 99%

Babesia microti-Group Parasites Compared Phylogenetically by Complete Sequencing of the CCT.ETA. Gene in 36 Isolates

et al. 2009

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ABSTRACT. Babesia microti, the erythroparasitic cause of human babesiosis, has long been taken to be a single species because classification by parasite morphology and host spectrum blurred distinctions between the parasites. Phylogenetic analyses of the 18S ribosomal RNA gene (18S rDNA) and, more recently, the β-tubulin gene have suggested inter-group heterogeneity. Intra-group relationships, however, remain unknown. This study was conducted to clarify the intra-and inter-group phylogenetic features of the B. microti-group parasites with the η subunit of the chaperonin-containing t-complex polypeptide l (CCTη) gene as a candidate genetic marker for defining the B. microti group. We prepared complete sequences of the CCTη gene from 36 piroplasms and compared the phylogenetic trees. The B. microti-group parasites clustered in a monophyletic assemblage separate from the Babesia sensu stricto and Theileria genera and subdivided predominantly into 4 clades (U.S., Kobe, Hobetsu, Munich) with highly significant evolutionary distances between the clades. B. rodhaini branched at the base of the B. microti-group parasites. In addition, a unique intron presence/absence matrix not observable in 18S rDNA or β-tubulin set the B. microti group entirely apart from either Babesia sensu stricto or Theileria. These results have strong implications for public health, suggesting that the B. microti-group parasites are a full-fledged genus comprising, for now, four core species, i.e., U.S., Kobe, Hobetsu, and Munich species nova. Furthermore, the CCTη gene is an instructive and definitive genetic marker for analyzing B. microti and related parasites.

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Babesia canis infection in canine-red blood cell-substituted SCID mice

Cited by 16 publications

References 12 publications

Epizootiologic Survey for <i>Babesia microti</i> among Small Wild Mammals in Northeastern Eurasia and a Geographic Deversity in the β-Tubulin Gene Sequences

Epizootiologic Survey for <i>Babesia microti</i> among Small Wild Mammals in Northeastern Eurasia and a Geographic Deversity in the β-Tubulin Gene Sequences

Apicoplast-Targeting Antibacterials Inhibit the Growth of Babesia Parasites

Babesia microti-Group Parasites Compared Phylogenetically by Complete Sequencing of the CCT.ETA. Gene in 36 Isolates

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Babesia canis infection in canine-red blood cell-substituted SCID mice

Cited by 16 publications

References 12 publications

Epizootiologic Survey for <i>Babesia microti</i> among Small Wild Mammals in Northeastern Eurasia and a Geographic Deversity in the &beta;-Tubulin Gene Sequences

Epizootiologic Survey for <i>Babesia microti</i> among Small Wild Mammals in Northeastern Eurasia and a Geographic Deversity in the &beta;-Tubulin Gene Sequences

Apicoplast-Targeting Antibacterials Inhibit the Growth of Babesia Parasites

Babesia microti-Group Parasites Compared Phylogenetically by Complete Sequencing of the CCT.ETA. Gene in 36 Isolates

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Epizootiologic Survey for <i>Babesia microti</i> among Small Wild Mammals in Northeastern Eurasia and a Geographic Deversity in the β-Tubulin Gene Sequences

Epizootiologic Survey for <i>Babesia microti</i> among Small Wild Mammals in Northeastern Eurasia and a Geographic Deversity in the β-Tubulin Gene Sequences