Bacillus aciditolerans sp. nov., isolated from paddy soil

Abstract

A Gram-stain-positive, motile, rod-shaped bacterial strain, YN-1 T , was isolated from a rice field in the town of Jietou, Yunnan Province, PR China. Colonies were circular, 1-2 mm in diameter, creamy white, with slightly irregular margins. The isolate grew optimally at 37 C, pH 7.0 and with 1.0 % (w/v) NaCl. On the basis of the results of 16S rRNA gene sequence similarity comparisons, YN-1 T clustered together with other species of the genus Bacillus and showed highest similarities with Bacillus onubensis 0911MAR22V3 T (98.0 %), Bacillus humi LMG22167 T (97.5 %), 'Bacillus timonensis' 10403023 (97.4 %) and 'Bacillus sinesaloumensis' P3516 (97.1 %). However, the DNA-DNA hybridization values between YN-1 T and closely related strains of species of the genus Bacillus were well below 47 %, indicating that they represent different taxa. The average nucleotide identity and the Genome-to-Genome Distance Calculator also revealed low relatedness (below 95 and 70 %, respectively) between YN-1 T and type strains of closely related species of the genus Bacillus. The DNA G+C content of the strain was 40 mol%. The major cellular fatty acids were iso-C 15 : 0 , anteiso-C 15 : 0 , and C 16 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, three unidentified aminophospholipids and two other unidentified lipids. Physiological and biochemical test results were also different from those of the most closely related species. On the basis of the phenotypic, genetic and chemotaxonomic data, strain YN-1 T is considered to represent a novel species of the genus Bacillus, for which the name Bacillus aciditolerans sp. nov. is proposed, with strain YN-1 T (=CCTCC AB 2017280 T =JCM 32973 T) as the type strain.

Robust demarcation of 17 distinct Bacillus species clades, proposed as novel Bacillaceae genera, by phylogenomics and comparative genomic analyses: description of Robertmurraya kyonggiensis sp. nov. and proposal for an emended genus Bacillus limiting it only to the members of the Subtilis and Cereus clades of species

“…The description of this species is the same as provided by Ding et al [106] for Bacillus aciditolerans.…”

“…The description of this species is the same as provided by Heyrman et al [105] for Bacillus humi. The description of this species is the same as provided by Ding et al [106] for Bacillus aciditolerans.…”

Development of an inexpensive matrix-assisted laser desorption—time of flight mass spectrometry method for the identification of endophytes and rhizobacteria cultured from the microbiome associated with maize

“…The exception was separation of the branch containing isolates that classified as Paenibacillus and Bacillus species, which bootstrapping supported only 76% of iterations. Within this Bacillales order, and in terms of rRNA gene sequences, isolate H1OZ89 showed high similarity to a phosphate solubilizing Paenibacillus species previously isolated from rape (Ding et al, 2019). Also, within this order, isolate H3OZ122 showed high similarity to a microplastics degrading Bacillus strain recently isolated from the Yellow Sea (Wang et al, 2019), isolate S4OZ125 showed high similarity to Bacillus aciditolerans, a recently described novel species isolated from a rice field (Ding et al, 2019) and H3OZ107 showed similarity to a Staphylococcus hominis strain isolated from a blood sample (Fig.…”

Assessment by Matrix-Assisted Laser Desorption – Time of Flight Mass Spectrometry of the Diversity of Endophytes and Rhizobacteria Cultured from the Maize Microbiome

“…The exception was separation of the branch containing isolates that classified as Paenibacillus and Bacillus species, which bootstrapping supported only 76% of iterations. Within this Bacillales order, and in terms of rRNA gene sequences, isolate H1OZ89 showed high similarity to a phosphate solubilizing Paenibacillus species previously isolated from rape (Ding et al 2019). Also, within this order, isolate H3OZ122 showed high similarity to a microplastics degrading Bacillus strain recently isolated from the Yellow Sea (Wang et al 2019), isolate S4OZ125 showed high similarity to…”

“…Bacillus aciditolerans, a recently described novel species isolated from a rice field (Ding et al 2019) and H3OZ107 showed similarity to a Staphylococcus hominis strain isolated from a blood sample (Fig. 4).…”