Bacillus acidoterrestris sp. nov., a New Thermotolerant Acidophile Isolated from Different Soils

Deinhard, G.; Blanz, Paul; Poralla, Karl; Altan, Erbülent

doi:10.1016/s0723-2020(87)80009-7

Cited by 168 publications

(101 citation statements)

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“…Physiological and chemotaxonomic characterization of strain ZJ-6 T was carried out with Bacillus acidocaldarius medium (BAM medium; Deinhard et al, 1987a). Gram-staining reactions were determined according to the method described by Gerhardt et al (1994).…”

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confidence: 99%

Alicyclobacillus aeris sp. nov., a novel ferrous- and sulfur-oxidizing bacterium isolated from a copper mine

Guo

You

Liu

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A novel mesophilic, acidophilic, endospore-forming bacterium, designated strain ZJ-6 T , was isolated from Zi-Jin copper mine in Inner Mongolia, China. Cells of strain ZJ-6 T were rod-shaped, stained Gram-positive or were Gram-variable, and grew aerobically at 25-35 6C (optimum, 30 6C) and pH 2.0-6.0 (optimum, pH 3.5). 16S rRNA gene sequence analysis showed that strain ZJ-6 T was related phylogenetically to members of the genus Alicyclobacillus, with 16S rRNA gene sequence similarities of 89.5-94.2 %. Cells contained MK-7 as the major quinone and the DNA G+C content was 51.2 mol%. Strain ZJ-6 T possessed a number of phenotypic characteristics that differentiated it from recognized Alicyclobacillus species, including its growth temperature, assimilation of various carbon sources, production of acids from a range of compounds, and the ability to grow chemoautotrophically using ferrous iron, elemental sulfur and tetrathionate as electron donors. The predominant cellular fatty acids of strain ZJ-6 T were anteiso-C 15 : 0 (67.1 %), iso-C 16 : 0 (7.7 %) and anteiso-C 17 : 0 (7.4 %); v-alicyclic fatty acids were not found. On the basis of these results, it is concluded that strain ZJ-6 T represents a novel species within the genus Alicyclobacillus, for which the name Alicyclobacillus aeris sp. nov. is proposed; the type strain is ZJ-6 T (5CGMCC 1.7072 T 5NBRC 104953 T ).

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confidence: 99%

Alicyclobacillus aeris sp. nov., a novel ferrous- and sulfur-oxidizing bacterium isolated from a copper mine

Guo

You

Liu

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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“…For experiments, strain K1 T was grown at 40 u C on modified Manning medium (Manning, 1975) Deinhard et al (1987a). Strain K1 T grew organotrophically in liquid and solid modified BAM that contained less yeast extract and glucose (to 0?2 and 0?5 g l 21 , respectively).…”

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confidence: 99%

Reclassification of ‘Sulfobacillus thermosulfidooxidans subsp. thermotolerans’ strain K1 as Alicyclobacillus tolerans sp. nov. and Sulfobacillus disulfidooxidans Dufresne et al. 1996 as Alicyclobacillus disulfidooxidans comb. nov., and emended description of the genus Alicyclobacillus

Karavaiko

Bogdanova

Tourova

et al. 2005

International Journal of Systematic and Evolutionary Microbiology

109

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Comparative analysis of 16S rRNA gene sequences, DNA–DNA hybridization data and phenotypic properties revealed that ‘Sulfobacillus thermosulfidooxidans subsp. thermotolerans’ strain K1 is not a member of the genus Sulfobacillus. Phylogenetically, strain K1 is closely related to unclassified strains of the genus Alicyclobacillus: the 16S rRNA gene sequence of strain K1 is similar to that of Alicyclobacillus sp. AGC-2 (99·6 %), Alicyclobacillus sp. 5C (98·9 %) and Alicyclobacillus sp. CLG (98·6 %) and bacterium GSM (99·1 %). The 16S rRNA gene sequence similarity values for strain K1 and species of the genus Alicyclobacillus with validly published names were in the range 92·1–94·6 %, and for S. thermosulfidooxidans VKM B-1269T the value was 87·7 %. Sulfobacillus disulfidooxidans SD-11T was also phylogenetically related to strain K1 (92·6 % sequence similarity) and thus belonged to the genus Alicyclobacillus. Chemotaxonomic data, such as the major cell-membrane lipid components of strains K1 and SD-11T (ω-alicyclic fatty acids) and the major isoprenoid quinone (menaquinone MK-7) of strain K1, supported the affiliation of strains K1 and SD-11T to the genus Alicyclobacillus. Physiological and molecular biological tests allowed genotypic and phenotypic differentiation of strains K1 and SD-11T from the nine Alicyclobacillus species with validly published names. The G+C content of the DNA of strain K1 was 48·7±0·6 mol%; that of strain SD-11T was 53±1 mol%. DNA–DNA reassociation studies showed low relatedness (22 %) between strains K1 and SD-11T, and even lower relatedness (3–5 %) between these strains and Alicyclobacillus acidocaldarius subsp. acidocaldarius ATCC 27009T, DSM 446T. DNA reassociation of strains K1 and SD-11T with Alicyclobacillus cycloheptanicus DSM 4006T gave values of 15 and 21, respectively. Based on the phenotypic and phylogenetic characteristics of strains K1 and SD-11T, Alicyclobacillus tolerans sp. nov. (type strain, K1T=VKM B-2304T=DSM 16297T) and Alicyclobacillus disulfidooxidans comb. nov. (type strain, SD-11T=ATCC 51911T=DSM 12064T) are proposed.

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“…1 mol% as Golovacheva and Karavaiko (16). Darland and Brock (7) and Deinhard et al (8,9). Except A. cycloheptanicus (see Table 6).…”

Section: Discussionmentioning

confidence: 97%

“…SD-11 exhibits subterminal, deforming spores. The analysis of the fatty acid composition in bacteria belonging to the genera Alicyclobacillus and Sulfobacillus revealed the pres- (8,9,36), which are rarely seen in other bacteria. The fatty acid composition for strain SD-11 closely resembles that of S. thermosuljidooxidans.…”

Section: Discussionmentioning

confidence: 99%

“…The new organism shows a net preference for organic sulfur substrates ( Table 6). The MI 50 CH system was also used to compare the utilization of carbohydrates by isolate SD-11 and by strains from the genus Alicyclobacillus, the major difference again being the lack of acidification observed after growth of isolate SD-11 (8,9). As a consequence of the differences in phenotypic and physiological characteristics, the isolate SD-11 may be classified as a new species of the genus Sulfobacillus.…”

Section: Discussionmentioning

confidence: 99%

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Sulfobacillus disulfidooxidans sp. nov., a New Acidophilic, Disulfide-Oxidizing, Gram-Positive, Spore-Forming Bacterium

Dufresne

Bousquet

Boissinot

et al. 1996

International Journal of Systematic Bacteriology

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An acidophilic, disulfide-oxidizing, mesophilic, aerobic bacterium was isolated from wastewater sludge. The new organism is a gram-positive sporulated rod. It can use elemental sulfur and pyrite as sole energy sources and grows on organic substrates such as glutamate and glucose. It also grows on the following organic sulfur substrates: oxidized and reduced glutathione, cysteine, cystine, and dithio(bis) benzothiazole and clearly shows a preference for disulfide bond-containing substrates. The optimal pH of growth is between 1.5 and 2.5, depending on the substrate used, and the growth temperature range varies from 4 to 40°C, with an optimal value at 35°C. The G+C chromosomal DNA content was measured at 53 A 1 mol%. Phylogenetic analysis of 16s genes coding for rRNA sequences places the new isolate in the genus Sulfobacillus. In addition, unique phenotypic and physiologic characteristics and DNA homology values assign the isolate to a new species in the genus. Therefore, this new isolate has been named Sulfobacillus disulJidooxidans and has been assigned ATCC number 51911.Many facultatively heterotrophic bacteria are known for their ability to grow under highly acidic conditions. Some of these within the genera Sulfolobus (5) and Acidianus (31) are extremely thermophilic archaebacteria. They obtain their energy by the oxidation or reduction of elemental sulfur or from organic substrates. Others are mesophilic gram-negative bacteria belonging to the genus Acidiphilium (2, 18) and are able to use organic substrates at pH 3.0 or belong to the genus Thiobacillus, such as Thiobacillus acidophilus (17), which is also known for its ability to grow with elemental sulfur or glucose at pH 2.5.However, the only acidophilic, facultatively autotrophic, gram-positive, spore-forming bacillus so far characterized is Sulfobacillus thermosu&dooxidans (16), together with two subspecies ("themzotolerans" and "asporogenes") that do not produce spores (19). Golovacheva and Karavaiko (16) first described the genus Sulfobacillus in 1978 as gram-positive, sporeforming bacteria growing on elemental sulfur, ferrous iron, and other metal sulfides. The rod-shaped cells were described as fairly pleomorphic. The spores were spherical or slightly oval, terminal, subterminal, or paracentral. S. therrnosulfdooxidans is a typical acidophilic, thermotolerant, and facultatively autotrophic bacterium. Since then, four isolates which are gramvariable, thermotolerant acidophiles have been recovered from mines: ALV (27), BC (27), C-MT1(15), and TH3 (4), and have been described as belonging presumably to this genus. Some other gram-positive bacteria from the genus Alicyclobacillus (7-9) can also grow under acidic conditions, but they are heterotrophic bacteria.This work reports on the isolation and characterization of a new facultatively autotrophic, mesophilic, acidophilic, grampositive, spore-forming bacterium (SD-11) that could belong to the genus Sulfobacillus. MATERIALS AND METHODSBacterial strains and culture conditions. The acidophilic strain SD-1...

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Bacillus acidoterrestris sp. nov., a New Thermotolerant Acidophile Isolated from Different Soils

Cited by 168 publications

References 13 publications

Alicyclobacillus aeris sp. nov., a novel ferrous- and sulfur-oxidizing bacterium isolated from a copper mine

Alicyclobacillus aeris sp. nov., a novel ferrous- and sulfur-oxidizing bacterium isolated from a copper mine

Reclassification of ‘Sulfobacillus thermosulfidooxidans subsp. thermotolerans’ strain K1 as Alicyclobacillus tolerans sp. nov. and Sulfobacillus disulfidooxidans Dufresne et al. 1996 as Alicyclobacillus disulfidooxidans comb. nov., and emended description of the genus Alicyclobacillus

Sulfobacillus disulfidooxidans sp. nov., a New Acidophilic, Disulfide-Oxidizing, Gram-Positive, Spore-Forming Bacterium

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