Bacillus aerius sp. nov., Bacillus aerophilus sp. nov., Bacillus stratosphericus sp. nov. and Bacillus altitudinis sp. nov., isolated from cryogenic tubes used for collecting air samples from high altitudes

Shivaji, Sisinthy; Chaturvedi, Preeti; Korpole, Suresh; Reddy, G. S. N.; Dutt, C. B. S.; Wainwright, Milton; Narlikar, J. V.; Bhargava, Poonam

doi:10.1099/ijs.0.64029-0

Cited by 255 publications

(188 citation statements)

References 24 publications

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“…Bacteria of the Bacillus pumilus clade are ubiquitous in a wide variety of terrestrial and marine environments, ranging from stratospheric air to deep-sea sediments and from soil to living beings (Shivaji et al, 2006;Liu et al, 2013;Branquinho et al, 2014). As a consequence of a proposal recommending to invalidate two species, Bacillus aerophilus and Bacillus stratosphericus (Branquinho et al, 2015;Liu et al, 2015a), and reclassification of Bacillus invictae as a later heterotypic synonym of Bacillus altitudinis (Liu et al, 2015b), the B. pumilus clade currently comprises four species with validly published names, B. pumilus, Bacillus safensis (Satomi et al, 2006), B. altitudinis (Shivaji et al, 2006) and Bacillus xiamenensis (Lai et al, 2014).…”

mentioning

confidence: 99%

“…As a consequence of a proposal recommending to invalidate two species, Bacillus aerophilus and Bacillus stratosphericus (Branquinho et al, 2015;Liu et al, 2015a), and reclassification of Bacillus invictae as a later heterotypic synonym of Bacillus altitudinis (Liu et al, 2015b), the B. pumilus clade currently comprises four species with validly published names, B. pumilus, Bacillus safensis (Satomi et al, 2006), B. altitudinis (Shivaji et al, 2006) and Bacillus xiamenensis (Lai et al, 2014). In a preliminary investigation of the phylogenetic diversity and marine ecotype of the B. pumilus clade using multilocus sequence analysis (Liu et al, 2013), strains DW5-4 T and NH7I_1 T were found to form two independent clades which were marked group C and group E, respectively.…”

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confidence: 99%

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Bacillus zhangzhouensis sp. nov. and Bacillus australimaris sp. nov.

Liu

Lai

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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Two strains, designated DW5-4 T and NH7I_1 T , were isolated from aquaculture water of a shrimp farm of Zhangzhou city in China and from surface sediment of the South China Sea, respectively. Cells of the strains were Gram-stain-positive, oxidase-and catalase-positive, rod-shaped and motile. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the two strains belonged to the Bacillus pumilus clade. 16S rRNA gene sequence similarity between strains DW5-4 T and NH7I_1 T was 99.8 %, and between each of the two strains and four type strains, B. pumilus ATCC 7061 T , Bacillus safensis FO-36b T , Bacillus altitudinis 41KF2b T and Bacillus xiamenensis HYC-10 T , were above 99.4 %. The digital DNA-DNA hybridization value between the two novel strains was 41.6 %, and between each of the two strains and the four above-mentioned type strains were below 53.0 %. In both strains, the principal fatty acids were iso-C 15 : 0 and anteiso-C 15 : 0 , and the isoprenoid quinone was identified as MK-7 (100 %). The DNA G+C contents of strains DW5-4 T and NH7I_1 T were 41.4 and 41.3 mol%, respectively. Based on phenotypic and genotypic characteristics, two novel species of the genus Bacillus are proposed: Bacillus zhangzhouensis sp. nov., with DW5-4 T (5MCCC 1A08372 T 5LMG 27144 T 5KCTC 33531 T ) as the type strain, and Bacillus australimaris sp. nov., with NH7I_1 T (5MCCC 1A05787 T 5LMG 27697 T 5KCTC 33532 T ) as the type strain.

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confidence: 99%

mentioning

confidence: 99%

Bacillus zhangzhouensis sp. nov. and Bacillus australimaris sp. nov.

Liu

Lai

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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“…The ubiquitous distribution of Bacillus species in nature is due to the resistance of their endospores to chemical and physical agents. Species of Bacillus have been shown to be resistant to radiation (Shivaji et al, 2006;Venkateswaran et al, 2003), extremely low temperatures (Brambilla et al, 2001;Logan et al, 2000;Rüger et al, 2000;Nelson & Parkinson, 1978;Abd El-Rahman et al, 2002;Lechner et al, 1998), high temperatures (Baker et al, 2001;Caccamo et al, 2000;Combet-Blanc et al, 1995), salinity Lee et al, 2006;Gugliandolo et al, 2003), alkalinity (Nogi et al, 2005;Vargas et al, 2005;Yumoto et al, 1998), acidity (Albert et al, 2005) and high pressure (Bae et al, 2005). Although Bacillus species have been isolated from a wide range of habitats, only one Bacillus species with a validly published name, Bacillus lehensis (Ghosh et al, 2007), has been isolated from the Himalayan region.…”

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confidence: 99%

Bacillus cecembensis sp. nov., isolated from the Pindari glacier of the Indian Himalayas

Reddy

Anarasi

Shivaji

2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Strain PN5 T is a Gram-positive, aerobic, motile, rod-shaped, peritrichously flagellated bacterium that was isolated from the Pindari glacier using nutrient agar medium. Cells of PN5 T are catalase-positive and oxidase-negative and contain lysine, glutamic acid and alanine in the peptidoglycan (peptidoglycan type A4a). Further, the cells are characterized by the presence of iso-C 15 : 0 and iso-C 16 : 1 as the predominant fatty acids and MK-7 as the isoprenoid quinone. Based on the above characteristics, strain PN5 T was assigned to the genus Bacillus. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain PN5 T clustered with the type strain of Bacillus silvestris with a sequence similarity of 97.2 %. DNA-DNA hybridization between PN5 T and B. silvestris DSM 12223 T resulted in a relatedness of only 15 %, clearly indicating that strain PN5 T represents a novel species. Further, PN5 T was different from B. silvestris with respect to various phenotypic and chemotaxonomic characteristics. Therefore, strain PN5 T is identified as a representative of a novel species of the genus Bacillus, for which the name Bacillus cecembensis sp. nov. is proposed. Bacillus cecembensis is unique among psychrotolerant Bacillus species in containing L-Lys-D-Glu in the cell-wall peptidoglycan. The type strain is PN5 T (5LMG 23935 T 5MTCC9127 T 5JCM 15113 T ).The genus Bacillus was created by Cohn (1872), with Bacillus subtilis as the type species. Members of the genus Bacillus are distinguished from other endospore-forming bacteria on the basis of being strict or facultative aerobes, rod-shaped, (usually) catalase-positive and they contain meso-diaminopimelic acid (meso-Dap) in the peptidoglycan, MK-7 as the major menaquinone and iso-C 15 : 0 and anteiso-C 15 : 0 as major fatty acids and have DNA G+C contents of 32-69 mol%. Interestingly, Bacillus is the only genus that contains organisms with L-lysine (Lee et al., 2006;Rheims et al., 1999) (Bae et al., 2005). Although Bacillus species have been isolated from a wide range of habitats, only one Bacillus species with a validly published name, Bacillus lehensis (Ghosh et al., 2007), has been isolated from the Himalayan region. In the present study, a unique psychrotolerant Bacillus strain (PN5 T ) containing L-lysine as the diamino acid in the peptidoglycan was isolated from a soil sample of the Pindari glacier of the Indian Himalayas; based on polyphasic taxonomic characterization, it is identified as representing a novel species.Strain PN5 T was isolated from a soil sample collected close to the Pindari glacier at an altitude of approximately 3500 m. Approximately 200 mg of the soil sample was suspended in 0.9 % saline, subjected to shaking for 1 h and allowed to settle and 100 ml was plated on nutrient agar plates [0.3 % beef extract, 0.5 % peptone, 0.8 % NaCl and 1.5 % agar (all w/v)]. The viable bacterial cell count was approximately 2.1610 5 c.f.u. and, based on colony morphology, 40 different morphotypes were observed. In the present study, one of the morp...

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“…Growth occurs between 8 and 45°C at pH 5-8. It tolerates up to 2% NaCl and degrades tyrosine, but tests negative for casein hydrolysis, urease, and phenylalanine deaminase activities, reduction of nitrate to nitrite, utilization of citrate and the Voges-Proskauer test [3].…”

Section: Bacteriamentioning

confidence: 99%

Study of Volcanic-Ash-Impregnated-Bacteria Filler to the Compressive Strength of Concrete

Purwanto

Nugroho

2017

MATEC Web Conf.

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Abstract. Technology of concrete has been advanced recently to improve the properties of concrete. Utilization of ash as the substitute of cement or filler of concrete also indicates good result in compressive strength concrete in certain amount. Harnessing the bacteria for self-healing concrete has also been successfully performed in order to maintain the destruction of the concrete by itself. Past research indicated that bacteria can be beneficiary to improve the compressive strength, absorption, permeability, and so on. In this paper, Bacillus Altitudinis was extracted from the karst (source from the limestone mountain) and bred through liquid media to be impregnated inside the volcanic ash. The impregnated bacteria in volcanic ash were used as the filler to the concrete mixture with the proportion of filler varied from 1%, 1.5% and 2% to the weight of cement. Mechanical properties of the concrete specimen showed that the compressive strength reached its best when the filler proportion was 1.5%. The absorption referred analogously to the increment of compressive strength due to the success of filling the void inside the concrete. Based on compression test to all the concrete specimens, there was enhancement about 18% and the average absorption can be abated around 23%. Future research due to some drawbacks turning up is the need of developing another media for bacteria to live and making sure the exact amount of bacteria impregnated to the media so that the desired advance of concrete compressive strength can be acquired.

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Bacillus aerius sp. nov., Bacillus aerophilus sp. nov., Bacillus stratosphericus sp. nov. and Bacillus altitudinis sp. nov., isolated from cryogenic tubes used for collecting air samples from high altitudes

Cited by 255 publications

References 24 publications

Bacillus zhangzhouensis sp. nov. and Bacillus australimaris sp. nov.

Bacillus zhangzhouensis sp. nov. and Bacillus australimaris sp. nov.

Bacillus cecembensis sp. nov., isolated from the Pindari glacier of the Indian Himalayas

Study of Volcanic-Ash-Impregnated-Bacteria Filler to the Compressive Strength of Concrete

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